目的:观察慢性束缚应激肝郁脾虚证模型大鼠中枢海马、杏仁核和外周胃、结肠组织脑肠肽酪神经肽(NPY)m RNA表达的变化及逍遥散对其影响。方法:将30只雄性SD大鼠随机分为正常对照组、模型组、逍遥散组,以慢性束缚应激方法制作肝郁脾虚证...目的:观察慢性束缚应激肝郁脾虚证模型大鼠中枢海马、杏仁核和外周胃、结肠组织脑肠肽酪神经肽(NPY)m RNA表达的变化及逍遥散对其影响。方法:将30只雄性SD大鼠随机分为正常对照组、模型组、逍遥散组,以慢性束缚应激方法制作肝郁脾虚证大鼠模型,并用逍遥散进行干预,造模21d结束后,采用实时-荧光定量PCR方法检测海马、杏仁核、胃、结肠组织NPY m RNA的表达。结果:与正常对照组比较,模型组大鼠NPY m RNA表达在海马显著降低(P<0.01),结肠组织显著升高(P<0.01);与模型组比较,逍遥散组大鼠NPY m RNA表达在海马显著升高(P<0.01),结肠组织表达显著降低(P<0.05)。结论:脑肠肽NPY可能参与了肝郁脾虚证的脑肠互动机制,逍遥散对中枢和外周NPY均有调节作用。展开更多
Objective： To determine the changes in the levels of endogenous metabolites in rats with chronic immobilization stress （CIS） taking Xiaoyao Powder （ XYP） and its modified prescription version, which lacks the vol...Objective： To determine the changes in the levels of endogenous metabolites in rats with chronic immobilization stress （CIS） taking Xiaoyao Powder （ XYP） and its modified prescription version, which lacks the volatile oils extracted from Herba Menthae. Methods： Twenty-four experimental male SD rats were randomly divided into 4 groups of 6 rats each： control, model, XYP-1 （containing volatile oils from Herba Menthae）, and XYP-2 （lacking volatile oils）. All rats except control group rats were subjected to CIS 3 h per day for 21 consecutive days. Groups XYP-1 and XYP-2 were given the extracted XYS with or without volatile oils （3.854 g/kg; suspended in distilled water） via gavage 1 h before CIS each day for 21 days. Rats were anesthetized using intraperitoneal injection of pentobarbital sodium （40 mg/kg） on the 22nd day. Observations were made using a Varian INOVA 600 MHz NMR spectrometer at 27 ~. Carr-PurcelI-Meiboom-Gill （CPMG） and longitudinal eddy-delay （LED） were applied, resulting in spectra showing only the signals from micro- and macro-metabolites. Results： Compared to controls, rats subjected to CIS showed increased levels of plasma metabolites, such as acetic acid, choline, N-glycoprotein （NAC）, saturated fatty acid, and blood sugars. Levels of low-density lipoprotein （LDL）, very low-density lipoprotein （VLDL）, and unsaturated fatty acids were decreased. The biochemical effects of XYS were characterized by elevated levels of VLDL, LDL, threonine, methionine, and glutamic acid in plasma. Conclusion： Some common and characteristic metabolites on the anti-CIS of XYP and its modified prescription were obtained. The metabolomics technology is a valuable tool and may be used to identify the specific metabolites and potential biomarkers of therapeutic effect of Chinese medicinal prescriptions.展开更多
In this study, Sprague-Dawley rats were immobilized to a frame for 3 hours a day for 21 days to establish a model of chronic immobilization stress. The body weight and food intake of rats subjected to chronic immobili...In this study, Sprague-Dawley rats were immobilized to a frame for 3 hours a day for 21 days to establish a model of chronic immobilization stress. The body weight and food intake of rats subjected to chronic immobilization stress were significantly decreased compared with the control group. Dual-labeling immunofluorescence revealed that the expression of leptin receptor and the co-localization coeffient in these leptic receptor neurons in the arcuate nucleus of the hypothalamus were both upregulated, while the number of neuropeptide Y neurons was decreased. Chronic immobilization stress induced high expression of leptin receptor in the arcuate nucleus and suppressed the synthesis and secretion of neuropeptide Y, thereby disrupting the pathways in the arcuate nucleus that regulate feeding behavior, resulting in diminished food intake and reduced body weight.展开更多
文摘目的:观察慢性束缚应激肝郁脾虚证模型大鼠中枢海马、杏仁核和外周胃、结肠组织脑肠肽酪神经肽(NPY)m RNA表达的变化及逍遥散对其影响。方法:将30只雄性SD大鼠随机分为正常对照组、模型组、逍遥散组,以慢性束缚应激方法制作肝郁脾虚证大鼠模型,并用逍遥散进行干预,造模21d结束后,采用实时-荧光定量PCR方法检测海马、杏仁核、胃、结肠组织NPY m RNA的表达。结果:与正常对照组比较,模型组大鼠NPY m RNA表达在海马显著降低(P<0.01),结肠组织显著升高(P<0.01);与模型组比较,逍遥散组大鼠NPY m RNA表达在海马显著升高(P<0.01),结肠组织表达显著降低(P<0.05)。结论:脑肠肽NPY可能参与了肝郁脾虚证的脑肠互动机制,逍遥散对中枢和外周NPY均有调节作用。
基金The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg 2012 ＊Supported by the National Natural Science Foundation of China （No. 30672578）, China National Funds for Distinguished Young Scientists （No. 30825046）, and Program for Innovative Research Team in Beijing University of Chinese Medicine （No. 2011CXTD-07）
文摘Objective： To determine the changes in the levels of endogenous metabolites in rats with chronic immobilization stress （CIS） taking Xiaoyao Powder （ XYP） and its modified prescription version, which lacks the volatile oils extracted from Herba Menthae. Methods： Twenty-four experimental male SD rats were randomly divided into 4 groups of 6 rats each： control, model, XYP-1 （containing volatile oils from Herba Menthae）, and XYP-2 （lacking volatile oils）. All rats except control group rats were subjected to CIS 3 h per day for 21 consecutive days. Groups XYP-1 and XYP-2 were given the extracted XYS with or without volatile oils （3.854 g/kg; suspended in distilled water） via gavage 1 h before CIS each day for 21 days. Rats were anesthetized using intraperitoneal injection of pentobarbital sodium （40 mg/kg） on the 22nd day. Observations were made using a Varian INOVA 600 MHz NMR spectrometer at 27 ~. Carr-PurcelI-Meiboom-Gill （CPMG） and longitudinal eddy-delay （LED） were applied, resulting in spectra showing only the signals from micro- and macro-metabolites. Results： Compared to controls, rats subjected to CIS showed increased levels of plasma metabolites, such as acetic acid, choline, N-glycoprotein （NAC）, saturated fatty acid, and blood sugars. Levels of low-density lipoprotein （LDL）, very low-density lipoprotein （VLDL）, and unsaturated fatty acids were decreased. The biochemical effects of XYS were characterized by elevated levels of VLDL, LDL, threonine, methionine, and glutamic acid in plasma. Conclusion： Some common and characteristic metabolites on the anti-CIS of XYP and its modified prescription were obtained. The metabolomics technology is a valuable tool and may be used to identify the specific metabolites and potential biomarkers of therapeutic effect of Chinese medicinal prescriptions.
基金supported by the National Natural Science Foundation of China,No.30672578,81072756and81202644China National Funds for Distinguished Young Scientists,No.30825046+2 种基金Program for Innovative Research Team in Beijing University of Chinese Medicine,No.2011CXTD-07Program for University Key Teacher of Hebei Medical UniversitySpecialized Research Fund for the Doctoral Program of Higher Education,No.20121323120016
文摘In this study, Sprague-Dawley rats were immobilized to a frame for 3 hours a day for 21 days to establish a model of chronic immobilization stress. The body weight and food intake of rats subjected to chronic immobilization stress were significantly decreased compared with the control group. Dual-labeling immunofluorescence revealed that the expression of leptin receptor and the co-localization coeffient in these leptic receptor neurons in the arcuate nucleus of the hypothalamus were both upregulated, while the number of neuropeptide Y neurons was decreased. Chronic immobilization stress induced high expression of leptin receptor in the arcuate nucleus and suppressed the synthesis and secretion of neuropeptide Y, thereby disrupting the pathways in the arcuate nucleus that regulate feeding behavior, resulting in diminished food intake and reduced body weight.
