Objective Probe into the influence and the mechanisms of CCK and gastrin on the apoptosis of bile duct carcinoma cells. Methods By taking beauvericin as the revulsant to the apoptosis of bile duct carcinoma cells, the...Objective Probe into the influence and the mechanisms of CCK and gastrin on the apoptosis of bile duct carcinoma cells. Methods By taking beauvericin as the revulsant to the apoptosis of bile duct carcinoma cells, the influence of CCK and gastrin on the apoptosis of bile duct carcinoma cells was investigated by using the techniques such as TUNEL fluorescent staining, stream mode cell detecting instrument and reverse bcl-2 oligonucleotide. Techniques of immunohistochemistry, in situ hybridization, flow cytometry (FCM) , RT-PCR were used to study the roles of apoptosis-related genes bcl-2 and baxResults After beauvericin 40 uM worked for 12 h, the survival rate of QBC939 bile duct carcinoma cells was decreased by 35% ?40% . About 80% of the bile duct carcinoma cells showed various degrees of apoptosis. CCK and gastrin could upregulate the threshold value of the apoptosis of bile duct carcinoma cells, which could be inhibited by L60, L18 and reverse bcl-2 oligonucleotide. In terms of both transcription and translating levels, CCK and gastrin could obviously promote the genetic expression of bcl-2, but had no influence on the genetic expression of bax. Addition of CCK-A receptor or CCK-B/gastr in receptor antagonist could remarkably inhibit the expression of bcl-2 boosted by gastrin-17 and CCK-8S.Conclusion CCK and gastrin inhibited the apoptosis of bile duct carcinoma cells through upregulating the genetic expression of bcl-2. Theoretically, this research has expanded our understanding to the mechanism of CCK and gastrin in controlling the growth of tumors, enriched our view to the mechanism of apoptosis of alimentary tract tumors, and has provided a new thinking for the assistant treatment to bile duct carcinoma cells as well.展开更多
基金Supported by National Natural Science Foundation of China.Project number:39670711
文摘Objective Probe into the influence and the mechanisms of CCK and gastrin on the apoptosis of bile duct carcinoma cells. Methods By taking beauvericin as the revulsant to the apoptosis of bile duct carcinoma cells, the influence of CCK and gastrin on the apoptosis of bile duct carcinoma cells was investigated by using the techniques such as TUNEL fluorescent staining, stream mode cell detecting instrument and reverse bcl-2 oligonucleotide. Techniques of immunohistochemistry, in situ hybridization, flow cytometry (FCM) , RT-PCR were used to study the roles of apoptosis-related genes bcl-2 and baxResults After beauvericin 40 uM worked for 12 h, the survival rate of QBC939 bile duct carcinoma cells was decreased by 35% ?40% . About 80% of the bile duct carcinoma cells showed various degrees of apoptosis. CCK and gastrin could upregulate the threshold value of the apoptosis of bile duct carcinoma cells, which could be inhibited by L60, L18 and reverse bcl-2 oligonucleotide. In terms of both transcription and translating levels, CCK and gastrin could obviously promote the genetic expression of bcl-2, but had no influence on the genetic expression of bax. Addition of CCK-A receptor or CCK-B/gastr in receptor antagonist could remarkably inhibit the expression of bcl-2 boosted by gastrin-17 and CCK-8S.Conclusion CCK and gastrin inhibited the apoptosis of bile duct carcinoma cells through upregulating the genetic expression of bcl-2. Theoretically, this research has expanded our understanding to the mechanism of CCK and gastrin in controlling the growth of tumors, enriched our view to the mechanism of apoptosis of alimentary tract tumors, and has provided a new thinking for the assistant treatment to bile duct carcinoma cells as well.