BACKGROUND: Cholecyst cholesterol lithiasis is a common disease of the digestive system; however, the cause of lithogenesis is still unclear. Although bile salt export pump (BSEP), multidrug resistance protein 2 (MRP2...BACKGROUND: Cholecyst cholesterol lithiasis is a common disease of the digestive system; however, the cause of lithogenesis is still unclear. Although bile salt export pump (BSEP), multidrug resistance protein 2 (MRP2), and multiple drug resistance 3 (MDR3 ) are 3 well-known transporting proteins, their effect on lithogenesis has not been elucidated. This study was undertaken to examine the relationship between BSEP, MRP2, MDR3, and cholesterol calculus formation. METHODS: Liver tissue specimens were taken from 20 patients with cholesterol calculus and from 10 patients with normal liver. mRNA and protein expressions of BSEP, MRP2, and MDR3 were determined by reverse tran-scriptase-polymerase chain reaction (RT-PCR) and Western blot, respectively. This study was approved by the ethics committee of China Medical University and informed consent was obtained from all patients. RESULTS: mRNA and protein expressions of BSEP, MRP2, and MDR3 were significantly down-regulated in the liver tissue of the patients with cholesterol calculus compared with normal liver tissue of the controls. CONCLUSION: The down-regulation of BSEP, MRP2, and MDR3 may be correlated with the formation of cholesterol calculus.展开更多
目的:探讨保护性通气在老年腹腔镜胆囊切除术中的应用效果。方法:2013年3月-2014年4月收治老年腹腔镜胆囊切除术患者60例,根据通气方式不同随机分成两组各30例,所有患者均采用全身麻醉间歇正压通气(IPPV),Ⅰ组患者潮气量设6~8 m L/kg,另...目的:探讨保护性通气在老年腹腔镜胆囊切除术中的应用效果。方法:2013年3月-2014年4月收治老年腹腔镜胆囊切除术患者60例,根据通气方式不同随机分成两组各30例,所有患者均采用全身麻醉间歇正压通气(IPPV),Ⅰ组患者潮气量设6~8 m L/kg,另加4~5 cm H2O的PEEP;Ⅱ组潮气量按标准8~10 m L/kg设定潮气量。结果:两组患者气腹前后气道峰值和Pa CO2比较差异有统计学意义(P<0.05),两组气腹后气道峰值比较差异有统计学意义(P<0.05),两组患者术前Ppeak、CVP、MAP、A-Ad O2、Pa CO2、Pa O2比较差异无统计学意义(P>0.05),插管30min后Ⅰ组与Ⅱ组比较差异有统计学意义(P<0.05)。结论:保护性通气是老年腹腔镜全麻腹腔镜胆囊切除术中安全、有效的通气方式,值得临床推广。展开更多
文摘BACKGROUND: Cholecyst cholesterol lithiasis is a common disease of the digestive system; however, the cause of lithogenesis is still unclear. Although bile salt export pump (BSEP), multidrug resistance protein 2 (MRP2), and multiple drug resistance 3 (MDR3 ) are 3 well-known transporting proteins, their effect on lithogenesis has not been elucidated. This study was undertaken to examine the relationship between BSEP, MRP2, MDR3, and cholesterol calculus formation. METHODS: Liver tissue specimens were taken from 20 patients with cholesterol calculus and from 10 patients with normal liver. mRNA and protein expressions of BSEP, MRP2, and MDR3 were determined by reverse tran-scriptase-polymerase chain reaction (RT-PCR) and Western blot, respectively. This study was approved by the ethics committee of China Medical University and informed consent was obtained from all patients. RESULTS: mRNA and protein expressions of BSEP, MRP2, and MDR3 were significantly down-regulated in the liver tissue of the patients with cholesterol calculus compared with normal liver tissue of the controls. CONCLUSION: The down-regulation of BSEP, MRP2, and MDR3 may be correlated with the formation of cholesterol calculus.
文摘目的:探讨保护性通气在老年腹腔镜胆囊切除术中的应用效果。方法:2013年3月-2014年4月收治老年腹腔镜胆囊切除术患者60例,根据通气方式不同随机分成两组各30例,所有患者均采用全身麻醉间歇正压通气(IPPV),Ⅰ组患者潮气量设6~8 m L/kg,另加4~5 cm H2O的PEEP;Ⅱ组潮气量按标准8~10 m L/kg设定潮气量。结果:两组患者气腹前后气道峰值和Pa CO2比较差异有统计学意义(P<0.05),两组气腹后气道峰值比较差异有统计学意义(P<0.05),两组患者术前Ppeak、CVP、MAP、A-Ad O2、Pa CO2、Pa O2比较差异无统计学意义(P>0.05),插管30min后Ⅰ组与Ⅱ组比较差异有统计学意义(P<0.05)。结论:保护性通气是老年腹腔镜全麻腹腔镜胆囊切除术中安全、有效的通气方式,值得临床推广。
