A novel detection method of a tumor marker alpha-fetoprotein（AFP） in human sera via capillary（electrophoresis） immunoassay coupled with chemiluminescence detection（CEIACL） was established.After a simple non-comp...A novel detection method of a tumor marker alpha-fetoprotein（AFP） in human sera via capillary（electrophoresis） immunoassay coupled with chemiluminescence detection（CEIACL） was established.After a simple non-competitive immunoassay procedure,seras were directly injected into capillary for electrophoresis with chemiluminescence reaction of luminol and H2O2 catalyzed by horseradish peroxidase and enhanced by（p-iodophenol）.The detection limit（S/N=3） of AFP is 0.85 μg/L.The CEIACL method proposed was successfully applied to the sensitive detection of AFP in human sera,which coincides with the results detected by Beckman automatic CL analyzer-access IA system.展开更多
A method of on\|line ultrasensitive chemiluminescence detection with capillary electrophoresis for Co(Ⅱ) is reported. Using our newly developed capillary electrophoresis with chemiluminescence detection system and no...A method of on\|line ultrasensitive chemiluminescence detection with capillary electrophoresis for Co(Ⅱ) is reported. Using our newly developed capillary electrophoresis with chemiluminescence detection system and novel mixing mode of the reagents, the effects of field\|amplified sample injection on detection limits of metal ions were studied in detail. The sub\|fmol/L level(0.13 fmol/L, 1.6 ymol) detection of cobalt ions in ultradilute solution was performed. The separation of fmol/L level Co(Ⅱ) and trace amounts of Ni(Ⅱ) was performed successfully.展开更多
文摘A novel detection method of a tumor marker alpha-fetoprotein（AFP） in human sera via capillary（electrophoresis） immunoassay coupled with chemiluminescence detection（CEIACL） was established.After a simple non-competitive immunoassay procedure,seras were directly injected into capillary for electrophoresis with chemiluminescence reaction of luminol and H2O2 catalyzed by horseradish peroxidase and enhanced by（p-iodophenol）.The detection limit（S/N=3） of AFP is 0.85 μg/L.The CEIACL method proposed was successfully applied to the sensitive detection of AFP in human sera,which coincides with the results detected by Beckman automatic CL analyzer-access IA system.
文摘A method of on\|line ultrasensitive chemiluminescence detection with capillary electrophoresis for Co(Ⅱ) is reported. Using our newly developed capillary electrophoresis with chemiluminescence detection system and novel mixing mode of the reagents, the effects of field\|amplified sample injection on detection limits of metal ions were studied in detail. The sub\|fmol/L level(0.13 fmol/L, 1.6 ymol) detection of cobalt ions in ultradilute solution was performed. The separation of fmol/L level Co(Ⅱ) and trace amounts of Ni(Ⅱ) was performed successfully.
