Significantly reduced tissue scattering of fluorescence signals in the second near-infrared(NIR-Ⅱ,1,000–1,700 nm)spectral region offers opportunities for large-depth in vivo bioimaging.Nowadays,most reported works c...Significantly reduced tissue scattering of fluorescence signals in the second near-infrared(NIR-Ⅱ,1,000–1,700 nm)spectral region offers opportunities for large-depth in vivo bioimaging.Nowadays,most reported works concerning NIR-II fluorescence in vivo bioimaging are realized by wide-field illumination and 2D-arrayed detection(e.g.,via InGaAs camera),which has high temporal resolution but limited spatial resolution due to out-of-focus signals.Combining NIR-II fluorescence imaging with confocal microscopy is a good approach to achieve high-spatial resolution visualization of biosamples even at deep tissues.In this presented work,a NIR-II fluorescence confocal microscopic system was setup.By using a kind of aggregation-induced emission(AIE)dots as NIR-II fluorescent probes,800 lm-deep 3D in vivo cerebrovascular imaging of a mouse was obtained,and the spatial resolution at 700 lm depth could reach 8.78 lm.Moreover,the time-correlated single photon counting(TCSPC)technique and femtosecond laser excitation were introduced into NIR-II fluorescence confocal microscopy,and in vivo confocal NIR-II fluorescence lifetime microscopic imaging(FLIM)of mouse cerebral vasculature was successfully realized.展开更多
文摘目的探讨急性缺血性脑卒中(AIS)动脉通畅性恶化(APD)的相关因素及预后。方法收集778例AIS患者的临床资料。根据是否发生APD将患者分为APD组及非APD组。于入院时和入院24 h对患者进行头颅MRI检查。采用NIHSS评分评价入院24 h神经功能,记录入院48 h的早期缺血性卒中复发率;于3个月和12个月采用mRS评分评估预后;记录第7 d、3个月、12个月的死亡率。结果与非APD组比较,APD组年龄、3个月mRS评分及高血压病史、入院前使用降压药、入院前使用降脂药、脑白质疏松的比率显著降低,入院及24 h NIHSS评分、12个月mRS评分及意识水平下降、颅内动脉狭窄、颅外动脉狭窄和侧支循环状态良好的比率显著升高(均P<0.05)。两组间TOAST分型的其他明确病因型卒中(SOE)及不明病因型卒中(SUE)差异有统计学意义(P=0.000,P=0.028)。Logistic回归分析显示,高血压病史(OR=0.46,95%CI:0.30-0.85,P=0.002)、入院NIHSS评分(OR=1.06,95%CI:1.03-1.11,P<0.001)、颅内动脉狭窄(OR=4.87,95%CI:3.02-15.21,P<0.001)、颅外动脉狭窄(OR=3.61,95%CI:1.59-6.37,P=0.011)和侧支循环状态良好(OR=3.17,95%CI:1.45-9.85,P=0.002)是APD的独立相关因素。与非APD组比较,APD组早期神经功能恶化、早期卒中复发比率显著升高(均P<0.001),第7 d、3个月、12个月的死亡率及3个月、12个月的预后不良率显著升高(均P<0.05)。结论入院NIHSS评分、颅内和颅外动脉狭窄及侧支循环状态良好与入院24 h APD独立相关,高血压病史具有保护作用。APD早期及长期预后均不佳。
基金supported by the National Natural Science Foundation of China(61735016)Zhejiang Provincial Natural Science Foundation of China(LR17F050001)
文摘Significantly reduced tissue scattering of fluorescence signals in the second near-infrared(NIR-Ⅱ,1,000–1,700 nm)spectral region offers opportunities for large-depth in vivo bioimaging.Nowadays,most reported works concerning NIR-II fluorescence in vivo bioimaging are realized by wide-field illumination and 2D-arrayed detection(e.g.,via InGaAs camera),which has high temporal resolution but limited spatial resolution due to out-of-focus signals.Combining NIR-II fluorescence imaging with confocal microscopy is a good approach to achieve high-spatial resolution visualization of biosamples even at deep tissues.In this presented work,a NIR-II fluorescence confocal microscopic system was setup.By using a kind of aggregation-induced emission(AIE)dots as NIR-II fluorescent probes,800 lm-deep 3D in vivo cerebrovascular imaging of a mouse was obtained,and the spatial resolution at 700 lm depth could reach 8.78 lm.Moreover,the time-correlated single photon counting(TCSPC)technique and femtosecond laser excitation were introduced into NIR-II fluorescence confocal microscopy,and in vivo confocal NIR-II fluorescence lifetime microscopic imaging(FLIM)of mouse cerebral vasculature was successfully realized.
