Tumor-associated myeloid cells constitute a series of plastic and heterogeneous cell populations within the tumor microenvironment(TME),and exhibit different phenotypes and functions in response to various microenviro...Tumor-associated myeloid cells constitute a series of plastic and heterogeneous cell populations within the tumor microenvironment(TME),and exhibit different phenotypes and functions in response to various microenvironmental signals.In light of promising preclinical data indicating that myeloid-based therapy can effectively suppress tumor growth,a series of novel immune-based therapies and approaches are currently undergoing clinical evaluation.A better understanding of the diversity and functional roles of different myeloid cell subtypes and of how they are associated with TME remodeling may help to improve cancer therapy.Herein,we focus on myeloid cells and discuss how tumor cells can simultaneously reprogram these cells through tumor-derived factors and metabolites.In addition,we discuss the interactions between myeloid cells and other cells in the TME that have the potential to directly or indirectly regulate tumor initiation,invasion,or angiogenesis.We further discuss the current and future potential applications of myeloid cells in the development of focused therapeutic strategies in cancer treatment.展开更多
The metastasis in which the cancer cells degrade the extracellular matrix （ECM） and invade to the sur- rounding and far tissues of the body is the leading cause of mortality in cancer patients, With a lot of advance...The metastasis in which the cancer cells degrade the extracellular matrix （ECM） and invade to the sur- rounding and far tissues of the body is the leading cause of mortality in cancer patients, With a lot of advancement in the field, yet the biological cause of metastasis are poorly understood, The microfluidic system provides advanced technology to reconstruct a variety of in vivo-like environment for studying the interactions between tumor ceils （TCs） and endothelial ceils （ECs）. This review gives a brief account of both two-dimensional models and three-dimensional microfluidic systems for the analysis of TCs-ECs co- culture as well as their applications to anti-cancer drug screening, Furthermore, the advanced methods for analyzing cell-to-cell interactions at single-cell level were also discussed,展开更多
Innate lymphoid cells(ILCs),a newly identified member of the lymphoid population,play a critical role in the transition from innate to adaptive immunity in host defense.ILCs are important in mucosal barrier immunity,t...Innate lymphoid cells(ILCs),a newly identified member of the lymphoid population,play a critical role in the transition from innate to adaptive immunity in host defense.ILCs are important in mucosal barrier immunity,tissue homeostasis,and immune regulation throughout the body.Significant alterations in ILC responses in lung diseases have been observed and reported.Emerging evidence has shown that ILCs are importantly involved in the pathogenesis and development of a variety of lung diseases,i.e.,helminth infections,allergic airway inflammation,and airway hyper-responsiveness.However,as a tissue-resident cell population,the role of ILCs in the lung remains poorly characterized.In this review,we discuss the role of ILCs in lung diseases,the mechanisms underlying the ILCmediated regulation of immunity,and the therapeutic potential of modulating ILC responses.展开更多
Varied behavior of luminophors phosphorescent life of rare earth terbium coordination compound as a molecular probe existing in different concentration quench of dioxygen and lipoid system was studied by phosphorescen...Varied behavior of luminophors phosphorescent life of rare earth terbium coordination compound as a molecular probe existing in different concentration quench of dioxygen and lipoid system was studied by phosphorescent spectrum. The terblum coordination compound probe was applied to study the interaction between rare earth and cells. The research results show that the molecular probe of terbium coordination compound was an effective probe for applying to the study of transmembrane process for rare earth ions.展开更多
The response of human osteoblast-like osteosarcoma cells （MG63） to surface modification of Ti-6Al-4V implant alloy was investigated by Laser Interference Lithography （LIL）. In this work, laser interference lithogr...The response of human osteoblast-like osteosarcoma cells （MG63） to surface modification of Ti-6Al-4V implant alloy was investigated by Laser Interference Lithography （LIL）. In this work, laser interference lithography was employed to fabricate the microstructures of grooves, dots and dimples onto the surfaces of Ti-6Al-4V samples. Two and three beam LIL systems were developed to carry out the experiments. The laser treatment resulted in the increases of the roughness and the contact angle of water on the implant alloy surfaces. The proliferation of osteoblasts was analyzed by MTT （3-（4,5-dirnethyl- 2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide） assay for the time periods of 4 hours, 2 days, 3 days, and 6 days. The MTT test results demonstrated that the laser treatment surfaces had a positive impact on the proliferation of os- teoblast cells after 24 hours. The alloy surface morphology and the morphological changes of MG63 cells cultured on the laser textured Ti-6Al-4V surface were observed by Scanning Electron Microscope （SEM）. The SEM results indicated that the os- teoblast cells were aligned on grooved surfaces and they were prolonged with the structures. Enzymatic detachment results showed that the 20 μm grooved structures provided the better cell adhesion to the textured Ti-6Al-4V surfaces.展开更多
基金the National Natural Science Foundation of China(No.82073145 to Y.Y.)Shanghai Pujiang Program(No.20PJ1412800 to Y.Y.)Natural Science Foundation of Shanghai(No.20ZR1472900 to Y.Y.).
文摘Tumor-associated myeloid cells constitute a series of plastic and heterogeneous cell populations within the tumor microenvironment(TME),and exhibit different phenotypes and functions in response to various microenvironmental signals.In light of promising preclinical data indicating that myeloid-based therapy can effectively suppress tumor growth,a series of novel immune-based therapies and approaches are currently undergoing clinical evaluation.A better understanding of the diversity and functional roles of different myeloid cell subtypes and of how they are associated with TME remodeling may help to improve cancer therapy.Herein,we focus on myeloid cells and discuss how tumor cells can simultaneously reprogram these cells through tumor-derived factors and metabolites.In addition,we discuss the interactions between myeloid cells and other cells in the TME that have the potential to directly or indirectly regulate tumor initiation,invasion,or angiogenesis.We further discuss the current and future potential applications of myeloid cells in the development of focused therapeutic strategies in cancer treatment.
基金financial support from National Natural Science Foundation of China (Nos. 214350002, 21727814 and 21621003)
文摘The metastasis in which the cancer cells degrade the extracellular matrix （ECM） and invade to the sur- rounding and far tissues of the body is the leading cause of mortality in cancer patients, With a lot of advancement in the field, yet the biological cause of metastasis are poorly understood, The microfluidic system provides advanced technology to reconstruct a variety of in vivo-like environment for studying the interactions between tumor ceils （TCs） and endothelial ceils （ECs）. This review gives a brief account of both two-dimensional models and three-dimensional microfluidic systems for the analysis of TCs-ECs co- culture as well as their applications to anti-cancer drug screening, Furthermore, the advanced methods for analyzing cell-to-cell interactions at single-cell level were also discussed,
基金supported by the USA National Institutes of Health Grant R01-HL-079669(J.F.)USA National Institutes of Health Grant R01-HL076179(J.F.)+2 种基金USA National Institutes of Health Grant R56-HL-123882(J.F.)USA VA Merit Award 1I01BX002729(J.F.)the Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents(Q.S.)
文摘Innate lymphoid cells(ILCs),a newly identified member of the lymphoid population,play a critical role in the transition from innate to adaptive immunity in host defense.ILCs are important in mucosal barrier immunity,tissue homeostasis,and immune regulation throughout the body.Significant alterations in ILC responses in lung diseases have been observed and reported.Emerging evidence has shown that ILCs are importantly involved in the pathogenesis and development of a variety of lung diseases,i.e.,helminth infections,allergic airway inflammation,and airway hyper-responsiveness.However,as a tissue-resident cell population,the role of ILCs in the lung remains poorly characterized.In this review,we discuss the role of ILCs in lung diseases,the mechanisms underlying the ILCmediated regulation of immunity,and the therapeutic potential of modulating ILC responses.
文摘Varied behavior of luminophors phosphorescent life of rare earth terbium coordination compound as a molecular probe existing in different concentration quench of dioxygen and lipoid system was studied by phosphorescent spectrum. The terblum coordination compound probe was applied to study the interaction between rare earth and cells. The research results show that the molecular probe of terbium coordination compound was an effective probe for applying to the study of transmembrane process for rare earth ions.
基金This work was supported by National Key Basic Research Program of China （973 Program No. 2012CB326406）, EU FP7 （BioRA）, China-EU H2020 （FabSurfWAR Nos. 2016YFE0112100 and 644971）, International Science and Technology Cooperation Program of China （No. 2012DFAlI070）, National Natural Science Foundation Program of China （Nos. 61176002, 11103047 and 11504030）, Jilin Provincial Science and Technology Program （20140414009GH, 20140622009JC, 20140414009GH, 20160520101JH, 20160101318JC and 20160623002TC）, and Frontier lnterdiscipline Program of Norman Bethune Health Science Center of Jilin University （No. 2013107025）.
文摘The response of human osteoblast-like osteosarcoma cells （MG63） to surface modification of Ti-6Al-4V implant alloy was investigated by Laser Interference Lithography （LIL）. In this work, laser interference lithography was employed to fabricate the microstructures of grooves, dots and dimples onto the surfaces of Ti-6Al-4V samples. Two and three beam LIL systems were developed to carry out the experiments. The laser treatment resulted in the increases of the roughness and the contact angle of water on the implant alloy surfaces. The proliferation of osteoblasts was analyzed by MTT （3-（4,5-dirnethyl- 2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide） assay for the time periods of 4 hours, 2 days, 3 days, and 6 days. The MTT test results demonstrated that the laser treatment surfaces had a positive impact on the proliferation of os- teoblast cells after 24 hours. The alloy surface morphology and the morphological changes of MG63 cells cultured on the laser textured Ti-6Al-4V surface were observed by Scanning Electron Microscope （SEM）. The SEM results indicated that the os- teoblast cells were aligned on grooved surfaces and they were prolonged with the structures. Enzymatic detachment results showed that the 20 μm grooved structures provided the better cell adhesion to the textured Ti-6Al-4V surfaces.
