Structured poly（dimethylsiloxane） （PDMS） chambers were designed and fabricated to enhance the signaling of human Embryonic Stem Cell （hESC） - derived neuronal networks on Microelectrode Array （MEA） platforms. ...Structured poly（dimethylsiloxane） （PDMS） chambers were designed and fabricated to enhance the signaling of human Embryonic Stem Cell （hESC） - derived neuronal networks on Microelectrode Array （MEA） platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than that in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.展开更多
设计了一种以STC89C51单片机为核心的数字化多功能电刺激器,可根据用户需要产生直流、方波、正弦波、三角波和锯齿波共5种类型的电刺激信号,同时可实现各个波形的幅值和频率可调,其幅值输出范围为0~5 V,频率范围为0~10 k Hz,实现相同温...设计了一种以STC89C51单片机为核心的数字化多功能电刺激器,可根据用户需要产生直流、方波、正弦波、三角波和锯齿波共5种类型的电刺激信号,同时可实现各个波形的幅值和频率可调,其幅值输出范围为0~5 V,频率范围为0~10 k Hz,实现相同温度、湿度及培养基环境条件下,细胞培养过程中的电信号刺激以及高通量筛选。展开更多
文摘Structured poly（dimethylsiloxane） （PDMS） chambers were designed and fabricated to enhance the signaling of human Embryonic Stem Cell （hESC） - derived neuronal networks on Microelectrode Array （MEA） platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than that in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.
文摘设计了一种以STC89C51单片机为核心的数字化多功能电刺激器,可根据用户需要产生直流、方波、正弦波、三角波和锯齿波共5种类型的电刺激信号,同时可实现各个波形的幅值和频率可调,其幅值输出范围为0~5 V,频率范围为0~10 k Hz,实现相同温度、湿度及培养基环境条件下,细胞培养过程中的电信号刺激以及高通量筛选。
