Thiophonate-methyl(TPM) is one of fungicides and pesticides widely used in agriculture field.However,the residue of its benzimidazole(BZD) metabolites in related agricultural products poses a potential risk to consume...Thiophonate-methyl(TPM) is one of fungicides and pesticides widely used in agriculture field.However,the residue of its benzimidazole(BZD) metabolites in related agricultural products poses a potential risk to consumers.In this paper,nickel oxide nanoparticle-deposited silica(SiO_2@NiO) composite was used for the selective enrichment and purification of TPM's BZD metabolites in celery cabbage sprayed with TPM.Meanwhile,high-performance liquid chromatography coupled with precursor ion scan-mass spectrometry(HPLC-PIS-MS) and high-resolution MS/MS analysis(HR-MS/MS) was utilized for their qualitative and quantitative analysis.Twenty-one potential TPM's BZD metabolites were found and four of them were identified.One metabolite was discovered for the first time.Besides,a robust and sensitive quantitative method was developed with good linearities(R^2> 0.9972) within a wide range of 10.00-1000 ng/g.The detection limits of three known TPM's metabolites were within the range of 3.20-4.90 ng/g.Relative standard deviations(RSDs) of intra-day and inter-day precisions were less than 18.3%,which showed perfect reproducibility.The method was successfully applied to monitoring TPM's BZD metabolites in celery cabbage sprayed with TPM and the concentration versus time curves of TPM's metabolites in celery cabbage were plotted.This method is expected to be used to monitor BZD residues in various fruits and vegetables.展开更多
A pot experiment and a sandy culture experiment grown with three vegetable crops of Chinese cabbage ( B. chinensis L., cv. Zao\|Shu 5), winter greens ( B.var.rosularis Tsen et Lee, cv. Shang\|Hai\|Qing) and cele...A pot experiment and a sandy culture experiment grown with three vegetable crops of Chinese cabbage ( B. chinensis L., cv. Zao\|Shu 5), winter greens ( B.var.rosularis Tsen et Lee, cv. Shang\|Hai\|Qing) and celery ( A.graveolens L. var. dulce DC., cv. Qing\|Qin) were conducted, respectively. The initial soil and four incubated soils with different extractable Cd (0.15, 0.89, 1.38, 1.84 and 2.30 mg Cd/kg soil) were used for the pot experiment. Five treatments were designed (0, 0.0625, 0.125, 0.250 and 0.500 mg Cd/L) in nutrient solution in the sandy culture experiment. Each treatment in pot and sandy culture experiments was trireplicated. The objectives of the study were to examine Cd accumulation in edible parts of selected vegetable crops, its correlation with Cd concentrations in vegetable garden soil or in nutrient solution, and evaluate the criteria of Cd pollution in vegetable garden soil and in nutrient solution based on the hygienic limit of Cd in vegetables. Cadmium concentrations in edible parts of the three selected vegetable crops were as follows: 0.01—0.15 mg/kg fresh weight for Chinese cabbage, 0.02—0.17 mg/kg fresh weight for winter greens, and 0.02—0.24 mg/kg fresh weight for celery in the pot experiment, and 0.1—0.4 mg/kg fresh weight for Chinese cabbage, 0.1—1.4 mg/kg fresh weight for winter greens, and 0.05—0.5 mg/kg fresh weight for celery in the pot experiment(except no\|Cd treatment). The order of the three test vegetable crops for cadmium accumulation in the edible parts was celery>winter greens>Chinese cabbage in both the pot experiment and the sandy culture experiment. Cadmium accumulation in edible parts or roots of the vegetable crops increased with increasing of cadmium concentration in the medium(soil or nutrient solution). And cadmium concentrations in edible parts of the test vegetable crops were significantly linearly related to the Cd levels in the growth media (soil and nutrient solution). Based on the regression equations established and the limit of cad展开更多
基金The National Natural Science Foundation of China(No.31671929,21635006 and 31670373)supported this work。
文摘Thiophonate-methyl(TPM) is one of fungicides and pesticides widely used in agriculture field.However,the residue of its benzimidazole(BZD) metabolites in related agricultural products poses a potential risk to consumers.In this paper,nickel oxide nanoparticle-deposited silica(SiO_2@NiO) composite was used for the selective enrichment and purification of TPM's BZD metabolites in celery cabbage sprayed with TPM.Meanwhile,high-performance liquid chromatography coupled with precursor ion scan-mass spectrometry(HPLC-PIS-MS) and high-resolution MS/MS analysis(HR-MS/MS) was utilized for their qualitative and quantitative analysis.Twenty-one potential TPM's BZD metabolites were found and four of them were identified.One metabolite was discovered for the first time.Besides,a robust and sensitive quantitative method was developed with good linearities(R^2> 0.9972) within a wide range of 10.00-1000 ng/g.The detection limits of three known TPM's metabolites were within the range of 3.20-4.90 ng/g.Relative standard deviations(RSDs) of intra-day and inter-day precisions were less than 18.3%,which showed perfect reproducibility.The method was successfully applied to monitoring TPM's BZD metabolites in celery cabbage sprayed with TPM and the concentration versus time curves of TPM's metabolites in celery cabbage were plotted.This method is expected to be used to monitor BZD residues in various fruits and vegetables.
文摘A pot experiment and a sandy culture experiment grown with three vegetable crops of Chinese cabbage ( B. chinensis L., cv. Zao\|Shu 5), winter greens ( B.var.rosularis Tsen et Lee, cv. Shang\|Hai\|Qing) and celery ( A.graveolens L. var. dulce DC., cv. Qing\|Qin) were conducted, respectively. The initial soil and four incubated soils with different extractable Cd (0.15, 0.89, 1.38, 1.84 and 2.30 mg Cd/kg soil) were used for the pot experiment. Five treatments were designed (0, 0.0625, 0.125, 0.250 and 0.500 mg Cd/L) in nutrient solution in the sandy culture experiment. Each treatment in pot and sandy culture experiments was trireplicated. The objectives of the study were to examine Cd accumulation in edible parts of selected vegetable crops, its correlation with Cd concentrations in vegetable garden soil or in nutrient solution, and evaluate the criteria of Cd pollution in vegetable garden soil and in nutrient solution based on the hygienic limit of Cd in vegetables. Cadmium concentrations in edible parts of the three selected vegetable crops were as follows: 0.01—0.15 mg/kg fresh weight for Chinese cabbage, 0.02—0.17 mg/kg fresh weight for winter greens, and 0.02—0.24 mg/kg fresh weight for celery in the pot experiment, and 0.1—0.4 mg/kg fresh weight for Chinese cabbage, 0.1—1.4 mg/kg fresh weight for winter greens, and 0.05—0.5 mg/kg fresh weight for celery in the pot experiment(except no\|Cd treatment). The order of the three test vegetable crops for cadmium accumulation in the edible parts was celery>winter greens>Chinese cabbage in both the pot experiment and the sandy culture experiment. Cadmium accumulation in edible parts or roots of the vegetable crops increased with increasing of cadmium concentration in the medium(soil or nutrient solution). And cadmium concentrations in edible parts of the test vegetable crops were significantly linearly related to the Cd levels in the growth media (soil and nutrient solution). Based on the regression equations established and the limit of cad
