Objective ： To investigate the effect of cancellous bone matrix gelatin （ BMG ） engineered with allogeneic chondrocytes in repairing articular cartilage defects in rabbits. Methods： Chondrocytes were seeded onto ...Objective ： To investigate the effect of cancellous bone matrix gelatin （ BMG ） engineered with allogeneic chondrocytes in repairing articular cartilage defects in rabbits. Methods： Chondrocytes were seeded onto threedimensional cancellous BMG and .cultured in vitro for 12 days to prepare BMG-chondrocyte complexes. Under anesthesia with 2.5% pentobarbital sodium （ lml/kg body weight）, articular cartilage defects were made on the right knee joints of 38 healthy New Zealand white rabbits （regardless of sex, aged 4-5 months and weighing 2. 5-3 kg） and the defects were then treated with 2.5% trypsin. Then BMG-chondrocyte complex （Group A, n = 18）, BMG （Group B, n =10）, and nothing （Group C, n =10） were implanted into the cartilage defects, respectively. The repairing effects were assessed by macroscopic, histologic, transmission electron microscopic （TEM） observation, immunohistochemical examination and in situ hybridization detection, respectively, at 2, 4, 8, 12 and 24 weeks after operation. Results.. Cancellous BMG was degraded within 8 weeks after operation. In Group A, lymphocyte infiltration was observed around the graft. At 24 weeks after operation, the cartilage defects were repaired by cartilagetissues and the articular cartilage and subchondral bone were soundly healed. Proteoglycan and type II collagen were detected in the matrix of the repaired tissues by Safranin-O staining and immunohistochemical staining, respectively. In situ hybridization proved gene expression of type II collagen in the cytoplasm of chondrocytes in the repaired tissues, TEM observation showed that chondrocytes and cartilage matrix in repaired tissues were almost same as those in the normal articular cartilage. In Group B, the defects were repaired by cartilage-fibrous tissues. In Group C, the defects were repaired only by fibrous tissues. Conclusions ： Cancellous BMG can be regarded as the natural cell scaffolds for cartilage tissue engineering. Articular cartilage defects can be repaired by canc展开更多
Background Cartilage oligomeric matrix protein （COMP） is mainly found in the skeletal system and vascular smooth muscle cells. Recent researches showed that it had a protective function on blood vessels and could al...Background Cartilage oligomeric matrix protein （COMP） is mainly found in the skeletal system and vascular smooth muscle cells. Recent researches showed that it had a protective function on blood vessels and could also inhibit vascular calcification. We investigated the serum COMPs in coronary heart disease （CHD） patients, and the relationship between serum COMP and the calcification of coronary artery. Methods A total of 233 consecutive chest pain patients who first underwent coronary angiography followed by multi-slice computed to- mography （MSCT） within six months were recruited and divided into two groups according to the coronary angiography luminal diameter narrowing percentages： CHD group （diameter narrowing 〉 50%, n = 194） and control group （diameter narrowing 〈 50%, n = 39）. The Gen- sini score, Syntax score and coronary artery calcium score （CACs） were calculated. The serum COMP level was determined using ELISA. Results The levels of COMP were significantly higher in the CHD group than in the control group 155.7 （124.5-194.5） ng/mL vs. 128.4 （113.0-159.9） ng/mL, P = 0.019. There were no correlation between COMP, Gensini score, Syntax score, severity of coronary stenosis and the number of coronary artery with stenosis 〉 50%. The serum COMP was correlated with age （r = 0.294, P 〈 0.001）, fasting glucose （r = 0.163, P = 0.015）, HbAlc （r = 0.194, P = 0.015） and CACs （r = 0.137, P = 0.037）. Stepwise linear regression analysis showed that COMP level and age were independent predictors of CACs in the CHD patients （fl = 0.402, t = 2.612, P = 0.015; fl = 0.472, t = 3.077, P = 0.005）. Performance of COMP for predicting CHD was shown as area under curve （AUC）： 0.632, 95% CI： 0.549-0.715 and upper tertile CACs was AUC： 0.602, 95% CI： 0.5264）.678 in receiver operating characteristic （ROC） curve analysis. Conclusion Calcification of coronary artery was an independent predictor of serum COMPs.展开更多
背景:p38丝裂原活化蛋白激酶信号转导通路属于丝裂原活化蛋白激酶家族成员,在骨关节炎的发生发展中发挥重要作用。目的:对骨关节炎病理进程中p38丝裂原活化蛋白激酶信号转导通路相关作用机制的研究进展进行综述。方法:由第一作者用计算...背景:p38丝裂原活化蛋白激酶信号转导通路属于丝裂原活化蛋白激酶家族成员,在骨关节炎的发生发展中发挥重要作用。目的:对骨关节炎病理进程中p38丝裂原活化蛋白激酶信号转导通路相关作用机制的研究进展进行综述。方法:由第一作者用计算机检索中国期刊全文数据库和PubMed数据库,检索词分别为"p38丝裂原活化蛋白激酶信号通路、骨关节炎、关节软骨、软骨细胞"和"p38MAPK signal transduction pathway,osteoarthritis,Articular cartilage,Chondrocyte"。从p38丝裂原活化蛋白激酶信号通路简介,p38丝裂原活化蛋白激酶在骨关节炎中的作用,p38丝裂原活化蛋白激酶阻断剂在骨关节炎中的应用3方面进行总结。共检索到可应用文献90篇,按纳入标准对文献进行筛选,共纳入46篇文章。结果与结论:p38丝裂原活化蛋白激酶信号通路与软骨细胞的肥大化和钙化、软骨细胞的凋亡、软骨基质金属蛋白酶的合成、软骨炎性细胞因子的产生等有密切关系,对骨关节炎的发生发展有重要影响。p38丝裂原活化蛋白激酶通过多种复杂的机制参与骨关节炎的形成和发展,对其起到极其重要的作用,因此阻断p38丝裂原活化蛋白激酶信号通路可能成为骨关节炎治疗的新靶点。展开更多
文摘Objective ： To investigate the effect of cancellous bone matrix gelatin （ BMG ） engineered with allogeneic chondrocytes in repairing articular cartilage defects in rabbits. Methods： Chondrocytes were seeded onto threedimensional cancellous BMG and .cultured in vitro for 12 days to prepare BMG-chondrocyte complexes. Under anesthesia with 2.5% pentobarbital sodium （ lml/kg body weight）, articular cartilage defects were made on the right knee joints of 38 healthy New Zealand white rabbits （regardless of sex, aged 4-5 months and weighing 2. 5-3 kg） and the defects were then treated with 2.5% trypsin. Then BMG-chondrocyte complex （Group A, n = 18）, BMG （Group B, n =10）, and nothing （Group C, n =10） were implanted into the cartilage defects, respectively. The repairing effects were assessed by macroscopic, histologic, transmission electron microscopic （TEM） observation, immunohistochemical examination and in situ hybridization detection, respectively, at 2, 4, 8, 12 and 24 weeks after operation. Results.. Cancellous BMG was degraded within 8 weeks after operation. In Group A, lymphocyte infiltration was observed around the graft. At 24 weeks after operation, the cartilage defects were repaired by cartilagetissues and the articular cartilage and subchondral bone were soundly healed. Proteoglycan and type II collagen were detected in the matrix of the repaired tissues by Safranin-O staining and immunohistochemical staining, respectively. In situ hybridization proved gene expression of type II collagen in the cytoplasm of chondrocytes in the repaired tissues, TEM observation showed that chondrocytes and cartilage matrix in repaired tissues were almost same as those in the normal articular cartilage. In Group B, the defects were repaired by cartilage-fibrous tissues. In Group C, the defects were repaired only by fibrous tissues. Conclusions ： Cancellous BMG can be regarded as the natural cell scaffolds for cartilage tissue engineering. Articular cartilage defects can be repaired by canc
文摘Background Cartilage oligomeric matrix protein （COMP） is mainly found in the skeletal system and vascular smooth muscle cells. Recent researches showed that it had a protective function on blood vessels and could also inhibit vascular calcification. We investigated the serum COMPs in coronary heart disease （CHD） patients, and the relationship between serum COMP and the calcification of coronary artery. Methods A total of 233 consecutive chest pain patients who first underwent coronary angiography followed by multi-slice computed to- mography （MSCT） within six months were recruited and divided into two groups according to the coronary angiography luminal diameter narrowing percentages： CHD group （diameter narrowing 〉 50%, n = 194） and control group （diameter narrowing 〈 50%, n = 39）. The Gen- sini score, Syntax score and coronary artery calcium score （CACs） were calculated. The serum COMP level was determined using ELISA. Results The levels of COMP were significantly higher in the CHD group than in the control group 155.7 （124.5-194.5） ng/mL vs. 128.4 （113.0-159.9） ng/mL, P = 0.019. There were no correlation between COMP, Gensini score, Syntax score, severity of coronary stenosis and the number of coronary artery with stenosis 〉 50%. The serum COMP was correlated with age （r = 0.294, P 〈 0.001）, fasting glucose （r = 0.163, P = 0.015）, HbAlc （r = 0.194, P = 0.015） and CACs （r = 0.137, P = 0.037）. Stepwise linear regression analysis showed that COMP level and age were independent predictors of CACs in the CHD patients （fl = 0.402, t = 2.612, P = 0.015; fl = 0.472, t = 3.077, P = 0.005）. Performance of COMP for predicting CHD was shown as area under curve （AUC）： 0.632, 95% CI： 0.549-0.715 and upper tertile CACs was AUC： 0.602, 95% CI： 0.5264）.678 in receiver operating characteristic （ROC） curve analysis. Conclusion Calcification of coronary artery was an independent predictor of serum COMPs.
文摘背景:p38丝裂原活化蛋白激酶信号转导通路属于丝裂原活化蛋白激酶家族成员,在骨关节炎的发生发展中发挥重要作用。目的:对骨关节炎病理进程中p38丝裂原活化蛋白激酶信号转导通路相关作用机制的研究进展进行综述。方法:由第一作者用计算机检索中国期刊全文数据库和PubMed数据库,检索词分别为"p38丝裂原活化蛋白激酶信号通路、骨关节炎、关节软骨、软骨细胞"和"p38MAPK signal transduction pathway,osteoarthritis,Articular cartilage,Chondrocyte"。从p38丝裂原活化蛋白激酶信号通路简介,p38丝裂原活化蛋白激酶在骨关节炎中的作用,p38丝裂原活化蛋白激酶阻断剂在骨关节炎中的应用3方面进行总结。共检索到可应用文献90篇,按纳入标准对文献进行筛选,共纳入46篇文章。结果与结论:p38丝裂原活化蛋白激酶信号通路与软骨细胞的肥大化和钙化、软骨细胞的凋亡、软骨基质金属蛋白酶的合成、软骨炎性细胞因子的产生等有密切关系,对骨关节炎的发生发展有重要影响。p38丝裂原活化蛋白激酶通过多种复杂的机制参与骨关节炎的形成和发展,对其起到极其重要的作用,因此阻断p38丝裂原活化蛋白激酶信号通路可能成为骨关节炎治疗的新靶点。
