Polymeric surfactants were prepared by ultrasonic copolymerization of carboxy methyl cellulose (CMC) and alkyl poly(etheroxy) acrylate. The adsorption mechanism of CMC based polymeric surfactants at silica gel/water i...Polymeric surfactants were prepared by ultrasonic copolymerization of carboxy methyl cellulose (CMC) and alkyl poly(etheroxy) acrylate. The adsorption mechanism of CMC based polymeric surfactants at silica gel/water interface was studied by the adsorption isotherms,IR difference spectra. The results show that the adsorptive amount goes up steadily with the increasing of copolymer concentration. Because of multicontact adsorption of copolymers at the surface of silica gel, the strong interaction between copolymers and surface of silica gel (such as hydrogen bond), large amount of polymeric surfactants adsorbs on silica gel. The pH value of copolymer solution plays a great role on adsorption, which indicates that the electrostatic action is the main adsorption mechanism of CMC based polymeric surfactants.展开更多
The micellar conformation of polymeric surfactants based on carboxy methyl cellulose in aqueous solution was studied by dynamic laser scattering and environmental scanning electron microscope. The results show that di...The micellar conformation of polymeric surfactants based on carboxy methyl cellulose in aqueous solution was studied by dynamic laser scattering and environmental scanning electron microscope. The results show that different polymeric surfactants have different micellar conformation. The micelles formed by copolymer A3, B7 and B9 are spheroidal or ellipsoidal. While copolymer A9 which is more hydrophilic forms rod like micelles. At high concentration the micelles in aqueous solution would aggregate and form network structure further. The dependence of micelle size distribution function F(R) on scattering vector confirms again that the micelles of A9 is not like spherical, while the independence of F(R) on scattering angles means B9 molecules forming sphere like micelles.展开更多
This study evaluates the influence of growth parameters such as pH, temperature, Carboxy Methyl Cellulose (CMC) concentration and agitation on cellulase production from three bacterial strains, viz., Achromobacter xyl...This study evaluates the influence of growth parameters such as pH, temperature, Carboxy Methyl Cellulose (CMC) concentration and agitation on cellulase production from three bacterial strains, viz., Achromobacter xylosoxidans BSS4, Bacillus sp. BSS3 and Pseudomonas sp. BSS2 isolated from the wood-yards on Kallai river bank in Kerala. Production of cellulase by these isolates was detected using basal salt medium (BSM) with 0.5% CMC as supplement, and CMCase activity was confirmed by iodine test. Dinitrosalicylic acid method was employed for assaying the cellulase production by measuring the amount of glucose liberated in μmol/mL/min. Maximum enzyme production from Pseudomonas sp. BSS2 was at pH 8, 37℃ with 1% CMC and 150 rpm, and cellulase production increased from initial 49.84 U/mL to 91.28 U/mL after optimization. The highest enzyme activity from Bacillus sp. BSS3 was at pH 9, 37℃ with 1% CMC, 150 rpm, and cellulase production increased from initial 26.05 U/mL to 104.68 U/mL after optimization. The maximum enzyme production from A. xylosoxidans BSS4 was at pH 7, 40℃ with 0.5% CMC and 150 rpm, and cellulase production increased from initial 55.28 U/mL to 68.37 U/mL after optimization. Thus among the three isolates, Bacillus sp. BSS3 showed maximum enzyme yield which can be explored for further scale up studies with an industrial perspective.展开更多
文摘Polymeric surfactants were prepared by ultrasonic copolymerization of carboxy methyl cellulose (CMC) and alkyl poly(etheroxy) acrylate. The adsorption mechanism of CMC based polymeric surfactants at silica gel/water interface was studied by the adsorption isotherms,IR difference spectra. The results show that the adsorptive amount goes up steadily with the increasing of copolymer concentration. Because of multicontact adsorption of copolymers at the surface of silica gel, the strong interaction between copolymers and surface of silica gel (such as hydrogen bond), large amount of polymeric surfactants adsorbs on silica gel. The pH value of copolymer solution plays a great role on adsorption, which indicates that the electrostatic action is the main adsorption mechanism of CMC based polymeric surfactants.
文摘The micellar conformation of polymeric surfactants based on carboxy methyl cellulose in aqueous solution was studied by dynamic laser scattering and environmental scanning electron microscope. The results show that different polymeric surfactants have different micellar conformation. The micelles formed by copolymer A3, B7 and B9 are spheroidal or ellipsoidal. While copolymer A9 which is more hydrophilic forms rod like micelles. At high concentration the micelles in aqueous solution would aggregate and form network structure further. The dependence of micelle size distribution function F(R) on scattering vector confirms again that the micelles of A9 is not like spherical, while the independence of F(R) on scattering angles means B9 molecules forming sphere like micelles.
文摘This study evaluates the influence of growth parameters such as pH, temperature, Carboxy Methyl Cellulose (CMC) concentration and agitation on cellulase production from three bacterial strains, viz., Achromobacter xylosoxidans BSS4, Bacillus sp. BSS3 and Pseudomonas sp. BSS2 isolated from the wood-yards on Kallai river bank in Kerala. Production of cellulase by these isolates was detected using basal salt medium (BSM) with 0.5% CMC as supplement, and CMCase activity was confirmed by iodine test. Dinitrosalicylic acid method was employed for assaying the cellulase production by measuring the amount of glucose liberated in μmol/mL/min. Maximum enzyme production from Pseudomonas sp. BSS2 was at pH 8, 37℃ with 1% CMC and 150 rpm, and cellulase production increased from initial 49.84 U/mL to 91.28 U/mL after optimization. The highest enzyme activity from Bacillus sp. BSS3 was at pH 9, 37℃ with 1% CMC, 150 rpm, and cellulase production increased from initial 26.05 U/mL to 104.68 U/mL after optimization. The maximum enzyme production from A. xylosoxidans BSS4 was at pH 7, 40℃ with 0.5% CMC and 150 rpm, and cellulase production increased from initial 55.28 U/mL to 68.37 U/mL after optimization. Thus among the three isolates, Bacillus sp. BSS3 showed maximum enzyme yield which can be explored for further scale up studies with an industrial perspective.
