Background:The aim of the study was to investigate the changes in the periarterial capillary-free zone(paCFZ)after anti-vascular endothelial growth factor(VEGF)therapy in patients with branch retinal vein occlusion(BR...Background:The aim of the study was to investigate the changes in the periarterial capillary-free zone(paCFZ)after anti-vascular endothelial growth factor(VEGF)therapy in patients with branch retinal vein occlusion(BRVO)by widefield swept-source optical coherence tomography angiography(SS-OCTA)and assess their associations with clinical outcomes.Methods:In this retrospective observational study of 54 treatment-naive BRVO patients with macular edema,we reviewed the findings of 12×12 mm^(2)SS-OCTA at baseline,3,6,and 12 months after intravitreal ranibizumab injections.The paCFZ and major retinal artery areas were measured on SS-OCTA images.The paCFZ area to artery area(P/A)ratio was calculated.Results:The paCFZ areas and P/A ratios of first-and second-order arteries were significantly greater in BRVO eyes than in contralateral eyes(all P<0.01),but there were no differences in the first-and second-order artery areas(P=0.20 and 0.25,respectively).The paCFZ areas and P/A ratios decreased significantly at 3,6,and 12 months after anti-VEGF therapy(all P<0.01).The baseline P/A ratio was significantly correlated with the baseline best-corrected visual acuity(BCVA),central retinal thickness,and their improvements at 3,6,and 12 months(all P<0.05).Baseline BCVA and P/A ratios of first-and second-order arteries were independently associated with the final BCVA in multivariate linear regression.Conclusions:Wide-field SS-OCTA shows that anti-VEGF therapy can lead to a significant improvement in the paCFZ parameters in BRVO.Smaller baseline P/A ratios on SS-OCTA tend to predict better visual outcomes at 12 months after anti-VEGF therapy.展开更多
本文通过测定塑化曲线确定聚乙烯(PE)的毛细管挂丝温度范围,测试了挤出原丝和低温区域拉伸丝的力学性能和热收缩行为。结果表明,低压下的毛细管挤出工艺对 PE 丝的力学性能影响很小,而低温区域拉伸能大幅度提高原丝的力学性能,产生自增...本文通过测定塑化曲线确定聚乙烯(PE)的毛细管挂丝温度范围,测试了挤出原丝和低温区域拉伸丝的力学性能和热收缩行为。结果表明,低压下的毛细管挤出工艺对 PE 丝的力学性能影响很小,而低温区域拉伸能大幅度提高原丝的力学性能,产生自增强效应,但热收缩率明显增大。展开更多
A simple, rapid and accurate capillary zone electrophoresis method was developed for the determination of chlorogenic acid as a marker in traditional Chinese medicines. The buffer solution comprised of 10 mmol/L sodiu...A simple, rapid and accurate capillary zone electrophoresis method was developed for the determination of chlorogenic acid as a marker in traditional Chinese medicines. The buffer solution comprised of 10 mmol/L sodium phosphate and 20 mmol/L boric acid containing 5% ethanol with the pH value adjusted to 7.00. The contents of chlorogenic acid in Flos Lonicerae and Flos Lonicerae containing herbal preparations were determined within 15 min.展开更多
Forming a new concentrated sense of useful components in conditions of nature, geological processes allows us to create techogenous deposits of required quality and forms in situ. The article presents a variant of the...Forming a new concentrated sense of useful components in conditions of nature, geological processes allows us to create techogenous deposits of required quality and forms in situ. The article presents a variant of the preparation of the deposit by a filtration geological process in situ. Structural and material transformation of the massif of hypergenesis nature methods allows bringing qualitative indicators to the required condition values. This will ensure the effective development of the deposit with traditional technological solutions. Experimental results of aqueous filtration are described. The schemes of technological solutions for natural and technogenic deposits are considered.展开更多
The pumping test in an unconfined aquifer with and without a low-permeability soil was studied experimentally to reveal the influence of the negative air pressure （NP） caused by the upper layer on the water content ...The pumping test in an unconfined aquifer with and without a low-permeability soil was studied experimentally to reveal the influence of the negative air pressure （NP） caused by the upper layer on the water content （w）, the water pressure （Pw）, as well as on the capillary pressure （Pc）. The study demonstrates that the NP generated in the vadose zone during pumping in the capper aquifer has a significant influence on w, Pw and Pc The Pc obtained from the capped aquifer is smaller than that without the upper layer. After the NP reaches a peak, the influence of the NP on Pc. is gradually declined as the air inflows through the upper layer which makes the NP gradually return to zero. When the air pressure returns to the atmospheric pressure, Pc in the vadose zone is only correlated with Pw, the same as the case with no upper layer.展开更多
Parkinson’s disease is the second most common neurodegenerative disease in the world.Beta-arrestin-2 has been reported to be an important protein involved in D2 dopamine receptor desensitization,which is essential to...Parkinson’s disease is the second most common neurodegenerative disease in the world.Beta-arrestin-2 has been reported to be an important protein involved in D2 dopamine receptor desensitization,which is essential to Parkinson’s disease.Moreover,the potential value of pharmacological inactivation of G protein-coupled receptor kinase or arrestin in the treatment of patients with Parkinson’s disease has recently been shown.We studied the interaction between D2 dopamine receptor and beta-arrestin-2 and the pharmacological regulation of chemical compounds on such interaction using capillary zone electrophoresis.The results from screening more than 40 compounds revealed three compounds that remarkably inhibit the beta-arrestin-2/D2 dopamine receptor interaction among them.These compounds are promising therapies for Parkinson’s disease,and the method used in this study has great potential for application in large-scale drug screening and evaluation.展开更多
Aim A capillary zone electrophoretic method （CZE） was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubat...Aim A capillary zone electrophoretic method （CZE） was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubated at 25 ℃ and then were separated by CZE with Tris-acetate buffer at pH 7.2. Each run could be accomplished within 5 min. Results In CZE, the peak width broadened due to the affinity interaction between natural products and thrombin. Compared with positive and negative control, the natural products （CB-1, CB-2） from Coreopsis tinctoria Nuttt. interacted with thrombin; CB-3 from Coreopsis tinctoria Nuttt. and HC-1, HC-2, HC-3 from Cistanche deserticola Ma. did not bind to thrombin. Both qualification and quantification characterizations of the binding were determined. Conclusion The established method is capable of sensitive and fast determination of natural products and thrombin interactions, it can be employed as an alternative method.展开更多
HIV-1 gains entry into target cells by sequentially interacting with cellular receptors and co-receptors. Both the receptor and co-receptor are recognized by HIV-1 envelope protein gpl20, which plays a key role in the...HIV-1 gains entry into target cells by sequentially interacting with cellular receptors and co-receptors. Both the receptor and co-receptor are recognized by HIV-1 envelope protein gpl20, which plays a key role in the entry process of HIV-1 into cells. The development of new inhibitors is essential since the viral enzyme reverse transcriptase （RT） is one of the first targets of antiretroviral therapy. It has been reported that a variety of natural plants, such as Artemisia rupestris L., have anti-viral pharmacological activity, and they might be the potential inhibitors of RT or V3 loop of gpl20 against HIV-1. RIQRGPGRAFVT1GK （R15K）, the relatively conserved region of V3 loop, can be used for binding research. In this work, we analyzed the interactions between different extracts from Artemisia rupestris L. and R15K by affinity capillary electrophoresis （ACE）. Moreover, we analyzed the interactions between different extracts from Artemisia rupestris L. and RT by capillary zone electrophoresis （CZE）. Our data showed that the chloroform extract ofArtemisia rupestris L. was active among the different plant extracts, which was consistent with previous studies. Taken together, our study provided a rapid screening method to seek anti-HIV ingredients in natural plants＇ extracts.展开更多
A method for the analysis of monosaccharides derived from glycoproteins and glycolipids was described using the newly developed technique of capillary zone electrophoresis(CZE).It is found that,at ca.0.5-5 nL in- ject...A method for the analysis of monosaccharides derived from glycoproteins and glycolipids was described using the newly developed technique of capillary zone electrophoresis(CZE).It is found that,at ca.0.5-5 nL in- jection,CZE can offer a high resolution of most monoses from glycoprotein and glycolipids.A mixture of amino-and non-aminomonosaccharides,which is difficult to separate with chromatographic methods,was separated complet- ely by CZE in borate buffer after the monosaccharides were derivatized with 1-naphthylamine.展开更多
A peptides migration model based on the principle of mechanics is presented in capillary zone electrophoresis (CZE). It is shown that the migration that the (tr) is a function of electric (Q), relative molecular mass ...A peptides migration model based on the principle of mechanics is presented in capillary zone electrophoresis (CZE). It is shown that the migration that the (tr) is a function of electric (Q), relative molecular mass (Mr), conformation parameter (Rc) of peptides and electrophoretic condition parameter(A). The conformation parameter is introduced to characterize multifarious shapes owing to the complex conlormation and the various kinds of macromolecules, where Rc≥1/3. The parameters A and Rc can be obtained from experimental data. The times of migration of the nine standard peptides were measured in pH 2.5buffer on different electrophoretic conditions in CZE. The experimental results agreed well with the theoretical prediction.展开更多
Capillary zone electrophoresis has been applied to the analysis of nucleotides. The effects of buffer concentration. pH and other operating conditions on the separation were investigated and optimized. By using the me...Capillary zone electrophoresis has been applied to the analysis of nucleotides. The effects of buffer concentration. pH and other operating conditions on the separation were investigated and optimized. By using the method, separation and identification of nuclotides in swine tissues were completed.展开更多
Coated capillary columns were prepared by sol-gel technology and used in the separation of basic proteins with capillary zone electrophoresis. The results indicated that a significant decrease in protein adsorption wa...Coated capillary columns were prepared by sol-gel technology and used in the separation of basic proteins with capillary zone electrophoresis. The results indicated that a significant decrease in protein adsorption was obtained and EOF was also diminished to zero in the pH range of 3-10.展开更多
Mycophenolic acid （MPA）, an immunosuppressive drug, was determined in human plasma by a simple capillary zone electrophoresis （CZE） method. A bare fused-silica capillary with an internal diameter （i.d.） of 75/am...Mycophenolic acid （MPA）, an immunosuppressive drug, was determined in human plasma by a simple capillary zone electrophoresis （CZE） method. A bare fused-silica capillary with an internal diameter （i.d.） of 75/am and an effective length of 50 cm was used for the separation. A 200 }aL plasma sample was deproteinized with 400 μL acetonitrile, and the supematant was directly injected into the capillary after a water plug at a pressure of 0.5 psi for 10 s. Boric acid （H3BO3, 200 mmol/L） solution adjusted to a pH of 8.60 with 1 mol/L sodium hydroxide （NaOH） solution was selected as the background electrolyte （BGE） through a uniform design. Calibration curve established on each day was linear over the MPA concentration range of 0.625-30.0 Bg/mL in human plasma with the correlation coefficient （r） larger than 0.9997. The limit of detection （LOD） was 0.200 ~tg/mL. Intra- and inter-day precisions at three concentrations within the calibration range were less than 4.45%. Plasma samples collected from 14 renal transplant recipients treated with the prodrug mycophenolate mofetil （MMF） were analyzed successfully by both the developed CZE technique and a validated high-performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） technique. The mean absolute and relative differences between the concentrations measured by the two methods were -0.17 and -0.20%, respectively. In conclusion, the CZE method described here was validated to be accurate, precise, and economical for the clinical quantification of MPA.展开更多
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.82101150,82171078)Shanghai clinical 3-year action plan-major clinical research(Grant Nos.SHDC2020CR2041B,SHDC2020CR5014-003)+1 种基金Shanghai Sailing Program(Grant No.21YF1405300)Shanghai Committee of Science and Technology(Grant Nos.20Y11911100,18411965100).
文摘Background:The aim of the study was to investigate the changes in the periarterial capillary-free zone(paCFZ)after anti-vascular endothelial growth factor(VEGF)therapy in patients with branch retinal vein occlusion(BRVO)by widefield swept-source optical coherence tomography angiography(SS-OCTA)and assess their associations with clinical outcomes.Methods:In this retrospective observational study of 54 treatment-naive BRVO patients with macular edema,we reviewed the findings of 12×12 mm^(2)SS-OCTA at baseline,3,6,and 12 months after intravitreal ranibizumab injections.The paCFZ and major retinal artery areas were measured on SS-OCTA images.The paCFZ area to artery area(P/A)ratio was calculated.Results:The paCFZ areas and P/A ratios of first-and second-order arteries were significantly greater in BRVO eyes than in contralateral eyes(all P<0.01),but there were no differences in the first-and second-order artery areas(P=0.20 and 0.25,respectively).The paCFZ areas and P/A ratios decreased significantly at 3,6,and 12 months after anti-VEGF therapy(all P<0.01).The baseline P/A ratio was significantly correlated with the baseline best-corrected visual acuity(BCVA),central retinal thickness,and their improvements at 3,6,and 12 months(all P<0.05).Baseline BCVA and P/A ratios of first-and second-order arteries were independently associated with the final BCVA in multivariate linear regression.Conclusions:Wide-field SS-OCTA shows that anti-VEGF therapy can lead to a significant improvement in the paCFZ parameters in BRVO.Smaller baseline P/A ratios on SS-OCTA tend to predict better visual outcomes at 12 months after anti-VEGF therapy.
文摘A simple, rapid and accurate capillary zone electrophoresis method was developed for the determination of chlorogenic acid as a marker in traditional Chinese medicines. The buffer solution comprised of 10 mmol/L sodium phosphate and 20 mmol/L boric acid containing 5% ethanol with the pH value adjusted to 7.00. The contents of chlorogenic acid in Flos Lonicerae and Flos Lonicerae containing herbal preparations were determined within 15 min.
文摘Forming a new concentrated sense of useful components in conditions of nature, geological processes allows us to create techogenous deposits of required quality and forms in situ. The article presents a variant of the preparation of the deposit by a filtration geological process in situ. Structural and material transformation of the massif of hypergenesis nature methods allows bringing qualitative indicators to the required condition values. This will ensure the effective development of the deposit with traditional technological solutions. Experimental results of aqueous filtration are described. The schemes of technological solutions for natural and technogenic deposits are considered.
基金Project supported by the National Natural Science Foundation of China(Grant No.41272251)the Research Grants Council of the Hong Kong Special Administrative Region,China(Grant No.HKU 701908P)
文摘The pumping test in an unconfined aquifer with and without a low-permeability soil was studied experimentally to reveal the influence of the negative air pressure （NP） caused by the upper layer on the water content （w）, the water pressure （Pw）, as well as on the capillary pressure （Pc）. The study demonstrates that the NP generated in the vadose zone during pumping in the capper aquifer has a significant influence on w, Pw and Pc The Pc obtained from the capped aquifer is smaller than that without the upper layer. After the NP reaches a peak, the influence of the NP on Pc. is gradually declined as the air inflows through the upper layer which makes the NP gradually return to zero. When the air pressure returns to the atmospheric pressure, Pc in the vadose zone is only correlated with Pw, the same as the case with no upper layer.
基金by the National Key Basic Research Foundation of China(Grant No.2006CB910301)the National Programs for High Technology Research and Development Program(863 Program)(No.2004AA404260)+1 种基金the National Natural Science Foundation of China(Grant Nos.30970599 and 30770421)the Fundamental Research Funds for the Central Universities of China(Grant No.1104006).
文摘Parkinson’s disease is the second most common neurodegenerative disease in the world.Beta-arrestin-2 has been reported to be an important protein involved in D2 dopamine receptor desensitization,which is essential to Parkinson’s disease.Moreover,the potential value of pharmacological inactivation of G protein-coupled receptor kinase or arrestin in the treatment of patients with Parkinson’s disease has recently been shown.We studied the interaction between D2 dopamine receptor and beta-arrestin-2 and the pharmacological regulation of chemical compounds on such interaction using capillary zone electrophoresis.The results from screening more than 40 compounds revealed three compounds that remarkably inhibit the beta-arrestin-2/D2 dopamine receptor interaction among them.These compounds are promising therapies for Parkinson’s disease,and the method used in this study has great potential for application in large-scale drug screening and evaluation.
文摘Aim A capillary zone electrophoretic method （CZE） was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubated at 25 ℃ and then were separated by CZE with Tris-acetate buffer at pH 7.2. Each run could be accomplished within 5 min. Results In CZE, the peak width broadened due to the affinity interaction between natural products and thrombin. Compared with positive and negative control, the natural products （CB-1, CB-2） from Coreopsis tinctoria Nuttt. interacted with thrombin; CB-3 from Coreopsis tinctoria Nuttt. and HC-1, HC-2, HC-3 from Cistanche deserticola Ma. did not bind to thrombin. Both qualification and quantification characterizations of the binding were determined. Conclusion The established method is capable of sensitive and fast determination of natural products and thrombin interactions, it can be employed as an alternative method.
基金National Natural Science Foundation(Grant No.81373372)Specialized Research Fund for the Doctoral Program of Higher Education of China(Grant No.20110001110021 and 20130001110059)
文摘HIV-1 gains entry into target cells by sequentially interacting with cellular receptors and co-receptors. Both the receptor and co-receptor are recognized by HIV-1 envelope protein gpl20, which plays a key role in the entry process of HIV-1 into cells. The development of new inhibitors is essential since the viral enzyme reverse transcriptase （RT） is one of the first targets of antiretroviral therapy. It has been reported that a variety of natural plants, such as Artemisia rupestris L., have anti-viral pharmacological activity, and they might be the potential inhibitors of RT or V3 loop of gpl20 against HIV-1. RIQRGPGRAFVT1GK （R15K）, the relatively conserved region of V3 loop, can be used for binding research. In this work, we analyzed the interactions between different extracts from Artemisia rupestris L. and R15K by affinity capillary electrophoresis （ACE）. Moreover, we analyzed the interactions between different extracts from Artemisia rupestris L. and RT by capillary zone electrophoresis （CZE）. Our data showed that the chloroform extract ofArtemisia rupestris L. was active among the different plant extracts, which was consistent with previous studies. Taken together, our study provided a rapid screening method to seek anti-HIV ingredients in natural plants＇ extracts.
基金This project is supported by National Natural Sience Foundation of China
文摘A method for the analysis of monosaccharides derived from glycoproteins and glycolipids was described using the newly developed technique of capillary zone electrophoresis(CZE).It is found that,at ca.0.5-5 nL in- jection,CZE can offer a high resolution of most monoses from glycoprotein and glycolipids.A mixture of amino-and non-aminomonosaccharides,which is difficult to separate with chromatographic methods,was separated complet- ely by CZE in borate buffer after the monosaccharides were derivatized with 1-naphthylamine.
文摘A peptides migration model based on the principle of mechanics is presented in capillary zone electrophoresis (CZE). It is shown that the migration that the (tr) is a function of electric (Q), relative molecular mass (Mr), conformation parameter (Rc) of peptides and electrophoretic condition parameter(A). The conformation parameter is introduced to characterize multifarious shapes owing to the complex conlormation and the various kinds of macromolecules, where Rc≥1/3. The parameters A and Rc can be obtained from experimental data. The times of migration of the nine standard peptides were measured in pH 2.5buffer on different electrophoretic conditions in CZE. The experimental results agreed well with the theoretical prediction.
文摘Capillary zone electrophoresis has been applied to the analysis of nucleotides. The effects of buffer concentration. pH and other operating conditions on the separation were investigated and optimized. By using the method, separation and identification of nuclotides in swine tissues were completed.
文摘Coated capillary columns were prepared by sol-gel technology and used in the separation of basic proteins with capillary zone electrophoresis. The results indicated that a significant decrease in protein adsorption was obtained and EOF was also diminished to zero in the pH range of 3-10.
基金Plan of Vitalizing Education Action Geared to the 21st Century(Plan 985)the 1st Phase Fund of Peking University 985 Project.
文摘Mycophenolic acid （MPA）, an immunosuppressive drug, was determined in human plasma by a simple capillary zone electrophoresis （CZE） method. A bare fused-silica capillary with an internal diameter （i.d.） of 75/am and an effective length of 50 cm was used for the separation. A 200 }aL plasma sample was deproteinized with 400 μL acetonitrile, and the supematant was directly injected into the capillary after a water plug at a pressure of 0.5 psi for 10 s. Boric acid （H3BO3, 200 mmol/L） solution adjusted to a pH of 8.60 with 1 mol/L sodium hydroxide （NaOH） solution was selected as the background electrolyte （BGE） through a uniform design. Calibration curve established on each day was linear over the MPA concentration range of 0.625-30.0 Bg/mL in human plasma with the correlation coefficient （r） larger than 0.9997. The limit of detection （LOD） was 0.200 ~tg/mL. Intra- and inter-day precisions at three concentrations within the calibration range were less than 4.45%. Plasma samples collected from 14 renal transplant recipients treated with the prodrug mycophenolate mofetil （MMF） were analyzed successfully by both the developed CZE technique and a validated high-performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） technique. The mean absolute and relative differences between the concentrations measured by the two methods were -0.17 and -0.20%, respectively. In conclusion, the CZE method described here was validated to be accurate, precise, and economical for the clinical quantification of MPA.
