目的建立同时测定制马钱子中4种生物碱士的宁、马钱子碱、士的宁氮氧化物、马钱子碱氮氧化物含量的反相离子对HPLC法。方法应用Lichrospher S C18色谱柱(4.6mm×300mm,5μm),以乙腈-水(38:62,每1000mL加入磷酸二氢钾3.4g和十二烷基...目的建立同时测定制马钱子中4种生物碱士的宁、马钱子碱、士的宁氮氧化物、马钱子碱氮氧化物含量的反相离子对HPLC法。方法应用Lichrospher S C18色谱柱(4.6mm×300mm,5μm),以乙腈-水(38:62,每1000mL加入磷酸二氢钾3.4g和十二烷基硫酸钠1.7g)为流动相,流速:1.0mL.min-1,柱温:30℃,检测波长:260nm。结果4种生物碱士的宁、马钱子碱、士的宁氮氧化物和马钱子碱氮氧化物的线性范围分别为13.7～219.1,12.55～200.8,0.3743～5.988和0.6225～9.96mg.L-1(r>0.9999),平均回收率分别为98.0%,102.2%,103.3%和99.9%(RSD均小于2.5%)。结论该方法简便、准确,重复性好,适用于制马钱子中士的宁、马钱子碱、士的宁氮氧化物、马钱子碱氮氧化物的含量测定。展开更多
目的用HPLC法测定马钱子膨化前后士的宁及马钱子碱氮氧化物的含量。方法 Diamonsil TM-C18柱(150 mm×4.6 mm,5μm),以乙腈(A)∶0.2%乙酸-0.2%三乙胺(B)为流动相,采用梯度洗脱的方法(0 min 8∶92;10 min 92∶8;30 min 79∶21;60 min...目的用HPLC法测定马钱子膨化前后士的宁及马钱子碱氮氧化物的含量。方法 Diamonsil TM-C18柱(150 mm×4.6 mm,5μm),以乙腈(A)∶0.2%乙酸-0.2%三乙胺(B)为流动相,采用梯度洗脱的方法(0 min 8∶92;10 min 92∶8;30 min 79∶21;60 min 40∶60),流速为1 mL/min,检测波长为254 nm。结果经膨化后,士的宁氮氧化物由0.0015上升到0.0035,上升率为233%,马钱子碱氮氧化物由0.0009上升到0.0025,上升率为278%。结论马钱子经膨化后,士的宁及马钱子碱氮氧化物的含量有所增加,实验结果为制定马钱子饮片的质量标准提供了一定的依据。展开更多
OBJECTIVE The alcoholism-related social problems have burdened the public health heavily.A better therapy for alcohol dependence as a chronic brain disease is highly required and interests the scientists worldwide. Ou...OBJECTIVE The alcoholism-related social problems have burdened the public health heavily.A better therapy for alcohol dependence as a chronic brain disease is highly required and interests the scientists worldwide. Our group has focused on screening the right drug with low toxicity and a sound curative effect from traditional Chinese medicine. METHODS Alcohol-preferring Fawn-Hooded(FH/Wjd) rat was used as an animal model of alcoholism to evaluate the effects of brucine N-oxide(BNO),an alkoloid naturally existing in the seeds of Strychnos nux-vomica L,on the alcohol-drinking behaviors.Furthermore,its adverse action and toxicity were investigated. RESULTS Treatment with BNO at the doses of 30,50 and 70 mg · kg^(-1)reduced the voluntary alcohol consumption and preference dosedependently and selectively without altering their water intake,total fluid intake,food consumption,body weight as well as sucrose preference. Remarkably,70 mg·kg^(-1)of BNO did suppress the deprivationinduced elevation of alcohol ingestion. Moreover,BNO used at the same doses as above had no influence on locomotion in an open field test and could not result in the place preference effect. CONCLUSION Taken together,BNO is of some significant pharmacological profiles to inhibit symptoms of alcohol dependence with high safety,and thence may be a potential pharmacotherapy.展开更多
文摘目的建立同时测定制马钱子中4种生物碱士的宁、马钱子碱、士的宁氮氧化物、马钱子碱氮氧化物含量的反相离子对HPLC法。方法应用Lichrospher S C18色谱柱(4.6mm×300mm,5μm),以乙腈-水(38:62,每1000mL加入磷酸二氢钾3.4g和十二烷基硫酸钠1.7g)为流动相,流速:1.0mL.min-1,柱温:30℃,检测波长:260nm。结果4种生物碱士的宁、马钱子碱、士的宁氮氧化物和马钱子碱氮氧化物的线性范围分别为13.7～219.1,12.55～200.8,0.3743～5.988和0.6225～9.96mg.L-1(r>0.9999),平均回收率分别为98.0%,102.2%,103.3%和99.9%(RSD均小于2.5%)。结论该方法简便、准确,重复性好,适用于制马钱子中士的宁、马钱子碱、士的宁氮氧化物、马钱子碱氮氧化物的含量测定。
文摘目的用HPLC法测定马钱子膨化前后士的宁及马钱子碱氮氧化物的含量。方法 Diamonsil TM-C18柱(150 mm×4.6 mm,5μm),以乙腈(A)∶0.2%乙酸-0.2%三乙胺(B)为流动相,采用梯度洗脱的方法(0 min 8∶92;10 min 92∶8;30 min 79∶21;60 min 40∶60),流速为1 mL/min,检测波长为254 nm。结果经膨化后,士的宁氮氧化物由0.0015上升到0.0035,上升率为233%,马钱子碱氮氧化物由0.0009上升到0.0025,上升率为278%。结论马钱子经膨化后,士的宁及马钱子碱氮氧化物的含量有所增加,实验结果为制定马钱子饮片的质量标准提供了一定的依据。
文摘OBJECTIVE The alcoholism-related social problems have burdened the public health heavily.A better therapy for alcohol dependence as a chronic brain disease is highly required and interests the scientists worldwide. Our group has focused on screening the right drug with low toxicity and a sound curative effect from traditional Chinese medicine. METHODS Alcohol-preferring Fawn-Hooded(FH/Wjd) rat was used as an animal model of alcoholism to evaluate the effects of brucine N-oxide(BNO),an alkoloid naturally existing in the seeds of Strychnos nux-vomica L,on the alcohol-drinking behaviors.Furthermore,its adverse action and toxicity were investigated. RESULTS Treatment with BNO at the doses of 30,50 and 70 mg · kg^(-1)reduced the voluntary alcohol consumption and preference dosedependently and selectively without altering their water intake,total fluid intake,food consumption,body weight as well as sucrose preference. Remarkably,70 mg·kg^(-1)of BNO did suppress the deprivationinduced elevation of alcohol ingestion. Moreover,BNO used at the same doses as above had no influence on locomotion in an open field test and could not result in the place preference effect. CONCLUSION Taken together,BNO is of some significant pharmacological profiles to inhibit symptoms of alcohol dependence with high safety,and thence may be a potential pharmacotherapy.
