含溴结构域蛋白2(bromodomain-containing protein 2,BRD2)具有两个串联的溴结构域和一个末端外结构域,是溴结构域和末端外结构域蛋白家族成员之一。BRD2蛋白能够特异性结合组蛋白乙酰化赖氨酸,参与基因的转录调控、染色质重塑和细胞增...含溴结构域蛋白2(bromodomain-containing protein 2,BRD2)具有两个串联的溴结构域和一个末端外结构域,是溴结构域和末端外结构域蛋白家族成员之一。BRD2蛋白能够特异性结合组蛋白乙酰化赖氨酸,参与基因的转录调控、染色质重塑和细胞增殖与凋亡等生物学活动。近年来研究表明,BRD2蛋白通过溴结构域和乙酰化组蛋白之间的表观遗传相互作用来调控基因转录和细胞周期、神经发育、炎症和脂肪生成,并且在肿瘤细胞增殖以及病毒感染和复制过程中也有着重要作用。该文主要从BRD2蛋白的结构特征、细胞功能和参与病毒复制的作用机制等方面进行综述,以期为深入研究BRD2蛋白的功能提供参考。展开更多
Objective:To study the correlation of bromodomain-containing protein 4(BRD4)and S-phase kinase-associated protein 2(Skp2)expression levels in ultrasound-guided thyroid nodule fine needle aspiration biopsy tissue with ...Objective:To study the correlation of bromodomain-containing protein 4(BRD4)and S-phase kinase-associated protein 2(Skp2)expression levels in ultrasound-guided thyroid nodule fine needle aspiration biopsy tissue with the pathological features of nodules.Methods:The tissues obtained in ultrasound-guided thyroid nodule fine needle aspiration biopsy in our hospital between March 2015 and March 2018 was selected and divided into malignant group and benign group according to the pathological results,and the expression levels of BRD4,Skp2,proliferation genes and angiogenesis genes were detected.Results:The BRD4 and Skp2 mRNA expression in thyroid nodules of the malignant group were significantly higher than those of the benign group,and the BRD4 and Skp2 mRNA expression in the malignant group of thyroid nodules with TNM III-IV,capsular invasion and lymph node metastasis were significantly higher than those in the thyroid nodules with TNM I-II,without capsular invasion and without lymph node metastasis;cyclin D1(CCND1),β-catenin,proliferation cell nuclear antigen(PCNA),vascular endothelial growth factor(VEGF),endothelial cell specific molecule-1(ESM-1),Survivin and cyclooxygenase 2(COX2)mRNA expression in thyroid nodules of the malignant group were obviously higher than those of the benign group and positively correlated with BRD4 and Skp2 while cyclin G2(CCNG2)and endostatin(ES)mRNA expression were significantly lower than those of the benign group and negatively correlated with BRD4 and Skp2.Conclusion:The high expression of BRD4 and Skp2 in malignant thyroid nodules is correlated with the pathological changes and can change the expression of proliferation genes and angiogenesis genes.展开更多
文摘含溴结构域蛋白2(bromodomain-containing protein 2,BRD2)具有两个串联的溴结构域和一个末端外结构域,是溴结构域和末端外结构域蛋白家族成员之一。BRD2蛋白能够特异性结合组蛋白乙酰化赖氨酸,参与基因的转录调控、染色质重塑和细胞增殖与凋亡等生物学活动。近年来研究表明,BRD2蛋白通过溴结构域和乙酰化组蛋白之间的表观遗传相互作用来调控基因转录和细胞周期、神经发育、炎症和脂肪生成,并且在肿瘤细胞增殖以及病毒感染和复制过程中也有着重要作用。该文主要从BRD2蛋白的结构特征、细胞功能和参与病毒复制的作用机制等方面进行综述,以期为深入研究BRD2蛋白的功能提供参考。
基金Hubei Provincial Natural Science Foundation Project.Project No:WJ2015MB125.
文摘Objective:To study the correlation of bromodomain-containing protein 4(BRD4)and S-phase kinase-associated protein 2(Skp2)expression levels in ultrasound-guided thyroid nodule fine needle aspiration biopsy tissue with the pathological features of nodules.Methods:The tissues obtained in ultrasound-guided thyroid nodule fine needle aspiration biopsy in our hospital between March 2015 and March 2018 was selected and divided into malignant group and benign group according to the pathological results,and the expression levels of BRD4,Skp2,proliferation genes and angiogenesis genes were detected.Results:The BRD4 and Skp2 mRNA expression in thyroid nodules of the malignant group were significantly higher than those of the benign group,and the BRD4 and Skp2 mRNA expression in the malignant group of thyroid nodules with TNM III-IV,capsular invasion and lymph node metastasis were significantly higher than those in the thyroid nodules with TNM I-II,without capsular invasion and without lymph node metastasis;cyclin D1(CCND1),β-catenin,proliferation cell nuclear antigen(PCNA),vascular endothelial growth factor(VEGF),endothelial cell specific molecule-1(ESM-1),Survivin and cyclooxygenase 2(COX2)mRNA expression in thyroid nodules of the malignant group were obviously higher than those of the benign group and positively correlated with BRD4 and Skp2 while cyclin G2(CCNG2)and endostatin(ES)mRNA expression were significantly lower than those of the benign group and negatively correlated with BRD4 and Skp2.Conclusion:The high expression of BRD4 and Skp2 in malignant thyroid nodules is correlated with the pathological changes and can change the expression of proliferation genes and angiogenesis genes.
