AIM:To investigate whether activated carbon nanoparticles suspension(ACNS) or methylene blue(MB) can increase the detected number of lymph nodes in colorectal cancer.METHODS:Sixty-seven of 72 colorectal cancer patient...AIM:To investigate whether activated carbon nanoparticles suspension(ACNS) or methylene blue(MB) can increase the detected number of lymph nodes in colorectal cancer.METHODS:Sixty-seven of 72 colorectal cancer patients treated at our hospital fulfilled the inclusion criteria of the study which was conducted from December 2010 to February 2012.Seven patients refused to participate.Eventually,60 patients were included,and randomly assigned to three groups(20 in each group):ACNS group(group A),MB group(group B) and non-stained conventional surgical group(group C).In group A,patients received subserosal injection of 1 mL ACNS in a 4-quadrant region around the mass.In group B,the main artery of specimen was identified and isolated after the specimen was removed,and 2 mL MB was slowly injected into the isolated,stretched and fixed vessel.In group C,no ACNS and MB were injected.All the mesentery lymph nodes were isolated and removed systematically by visually inspecting and palpating the adipose tissue.RESULTS:No difference was observed among the three groups in age,gender,tumor location,tumor diameter,T-stage,degree of differentiation,postoperative complications and peritoneal drainage retention time.The total number of detected lymph nodes was 535,476 and 223 in the three groups,respectively.The mean number of detected lymph nodes per patient was significantly higher in group A than in group C(26.8 ± 8.4 vs 12.2 ± 3.2,P < 0.001).Similarly,there were significantly more lymph nodes detected in group B than in group C(23.8 ± 6.9 vs 12.2 ± 3.2,P < 0.001).However,there was no significant difference between group A and group B.There were 50,46 and 32 metastatic lymph nodes dissected in 13 patients of group A,10 patients of group B and 11 patients of group C,without significant differences among the three groups.Eleven of the 60 patients had insufficient number of detected lymph nodes(< 12).Only one patient with T 4a rectal cancer had 10 lymph nodes detected in group B,the other 10 patients were all from group C.Based on展开更多
The plasma membrane （PM） H^＋-ATPase is an important ion pump in the plant cell membrane. By extruding protons from the cell and generating a membrane potential, this pump energizes the PM, which is a prerequisite f...The plasma membrane （PM） H^＋-ATPase is an important ion pump in the plant cell membrane. By extruding protons from the cell and generating a membrane potential, this pump energizes the PM, which is a prerequisite for growth. Modification of the autoinhibitory terminal domains activates PM H^＋-ATPase activity, and on this basis it has been hypothesized that these regulatory termini are targets for physiological factors that activate or inhibit proton pumping. In this review, we focus on the posttranslational regulation of the PM H＋-ATPase and place regulation of the pump in an evolutionary and physiological context. The emerging picture is that multiple signals regulating plant growth interfere with the posttranslational regulation of the PM H^＋-ATPase.展开更多
A series of chitosan-g-poly （acrylic acid）/vermiculite hydrogel composites were synthesized and used as adsorbents for the investigation of the effect of process parameters such as vermiculite content, pH of dye sol...A series of chitosan-g-poly （acrylic acid）/vermiculite hydrogel composites were synthesized and used as adsorbents for the investigation of the effect of process parameters such as vermiculite content, pH of dye solution, contact time, initial concentration of dye solution, temperature, ionic strength and concentration of surfactant sodium dodecyl sulfate on the removal of Methylene Blue （MB） from aqueous solution. The results showed that the adsorption capacity for dye increased with increasing pH, contact time and initial dye concentration, but decreased with increasing temperature, ionic strength and sodium dodecyl sulfate concentration in the present of the surfactant. The adsorption kinetics of MB onto the hydrogel composite followed pseudo second-order kinetics and the adsorption equilibrium data obeyed Langmuir isotherm. By introducing 10 wt.% vermiculite into chitosan-g-poly （acrylic acid） polymeric network, the obtaining hydrogel composite showed the highest adsorption capacity for MB, and then could be regarded as a potential adsorbent for cationic dye removal in a wastewater treatment process.展开更多
Owing to the worldwide abundance and low-cost of Na, room-temperature Na-ion batteries are emerging as attractive energy storage systems for large- scale grids. Increasing the Na content in cathode materials is one of...Owing to the worldwide abundance and low-cost of Na, room-temperature Na-ion batteries are emerging as attractive energy storage systems for large- scale grids. Increasing the Na content in cathode materials is one of the effective ways to achieve high energy density. Prussian blue and its analogues （PBAs） are promising Na-rich cathode materials since they can theoretically store two Na＋ ions per formula unit. However, increasing the Na content in PBAs cathode materials remains a major challenge. Here we show that sodium iron hexacyanoferrate with high Na content can be obtained by simply controlling the reducing agent and reaction atmosphere during synthesis. The Na content can reach as high as 1.63 per formula, which is the highest value for sodium iron hexacyanoferrate. This Na-rich sodium iron hexacyanoferrate demonstrates a high specific capacity of 150 mAh·g^-1 and remarkable cycling performance with 90% capacity retention after 200 cycles. Furthermore, the Na intercalation/ de-intercalation mechanism has been systematically studied by in situ Raman spectroscopy, X-ray diffraction and X-ray absorption spectroscopy analysis for the first time. The Na-rich sodium iron hexacyanoferrate can function as a plenteous Na reservoir and has great potential as a cathode material for practical Na-ion batteries.展开更多
AIM:To investigate the predictive value of narrowband imaging with magnifying endoscopy (NBI-ME) for identifying gastric intestinal metaplasia (GIM) in unselected patients. METHODS:We prospectively evaluated consecuti...AIM:To investigate the predictive value of narrowband imaging with magnifying endoscopy (NBI-ME) for identifying gastric intestinal metaplasia (GIM) in unselected patients. METHODS:We prospectively evaluated consecutive patients undergoing upper endoscopy for various indications, such as epigastric discomfort/pain, anaemia, gastro-oesophageal reflux disease, suspicion of peptic ulcer disease, or chronic liver diseases. Patients underwent NBI-ME, which was performed by three blinded, experienced endoscopists. In addition, five biopsies (2 antrum, 1 angulus, and 2 corpus) were taken and examined by two pathologists unaware of the endoscopic findings to determine the presence or absence of GIM. The correlation between light blue crest (LBC) appearance and histology was measured. Moreover, we quantified the degree of LBC appearance as less than 20% (+), 20%-80% (++) and more than 80% (+++) of an image field, and the semiquantitative evaluation of LBC appearance was correlated with IM percentage from the histological findings. RESULTS:We enrolled 100 (58 F/42 M) patients who were mainly referred for gastro-esophageal reflux disease/dyspepsia (46%), cancer screening/anaemia (34%), chronic liver disease (9%), and suspected celiac disease (6%); the remaining patients were referred for other indications. The prevalence of Helicobacter pylori (H. pylori ) infection detected from the biopsies was 31%, while 67% of the patients used proton pump inhibitors. LBCs were found in the antrum of 33 patients (33%); 20 of the cases were classified as LBC+, 9 as LBC++, and 4 as LBC+++. LBCs were found in the gastric body of 6 patients (6%), with 5 of them also having LBCs in the antrum. The correlation between the appearance of LBCs and histological GIM was good, with a sensitivity of 80% (95%CI:67-92), a specificity of 96% (95%CI:93-99), a positive predictive value of 84% (95%CI:73-96), a negative predictive value of 95% (95%CI:92-98), and an accuracy of 93% (95%CI:90-97). The NBI-ME examination overlooked GIM in 8 cases, but the GIM展开更多
Group size and composition of blue sheep(Pseudois nayaur)were studied in the Helan Mountains, Ningxia Autonomous Region from November to December 2003.We scanned mountain slopes with binoculars and observed with 20-60...Group size and composition of blue sheep(Pseudois nayaur)were studied in the Helan Mountains, Ningxia Autonomous Region from November to December 2003.We scanned mountain slopes with binoculars and observed with 20-60× spotting-scope. A total of 310 herds of blue sheep and 1 336 individuals were observed during the study period. Blue sheep were frequently seen in small herds of 2 to 8 individuals, which represent 94.8% of total herds observed. Herds consisting of 9 individuals or more represented 5.2%. The largest herd we observed numbered 51 individuals. Mean group size was 4.2 individuals. Blue sheep herds can be divided into three types: male herds (composed solely of males), female herds (consisting of females with or without juveniles of both sexes), and mixed herds (including adult males, females, and subadults).Among the 310 herds, female herds were counted 150 times(48.4%), mixed herds 154 times(49.7%), and male herds 6 times(1.9%). Of 1 336 blue sheep classified by sex and age, adults, subadults and juveniles composed 64.1%,20.8%, and 15.1% respectively. The female:male ratio of adults was 1∶0.73. The ratio of adult females to juveniles was 1∶0.56, which is higher than the ratio recorded in spring (1∶0.43) or summer (1∶0.44).The results showed that the Helan Mountains State Nature Reserve has succeed in protecting blue sheep.展开更多
The novel CuO-SnO2 nanocomposite oxide photocatalysts were prepared by simple co-precipitation method, and characterized by X- ray diffraction, transmission electron microscopy, N2 adsorption-desorption measurement an...The novel CuO-SnO2 nanocomposite oxide photocatalysts were prepared by simple co-precipitation method, and characterized by X- ray diffraction, transmission electron microscopy, N2 adsorption-desorption measurement and UV-Vis diffuse reflectance spectroscopy. The photocatalytic activities of CuO-SnO2, evaluated using the photodegradation of Acid Blue 62 as a probe reaction under the irradiation of Xenon light, were also found to be related to the calcination temperature and the molar ratio of Cu to Sn. The maximum photocatalytic activity of the CuO-SnO2 photocatalyst was observed to be calcined at 500~C for 3 h （the molar ratio of Cu to Sn was 1：1） due to the sample with good crystallization and high surface area. It also showed much higher photocatalytic activity in treatment dye wastewater under simulated sunlight irradiation compared to Degussa P25 TiO2.展开更多
Attapulgite（APT） has been frequently used for the adsorptive removal of dyes from aqueous solution owing to its unique one-dimensional nanoscale structure and low-cost, abundant,eco-friendly advantages. In this work...Attapulgite（APT） has been frequently used for the adsorptive removal of dyes from aqueous solution owing to its unique one-dimensional nanoscale structure and low-cost, abundant,eco-friendly advantages. In this work, APT was functionalized under mild hydrothermal condition using chloroacetic acid（CA） with ／COOH functional groups to improve its adsorption properties. The effect of hydrothermal modification on the microstructure and physicochemical features of APT was investigated by Fourier transform infrared spectroscopy, X-ray diffraction and Field-emission scanning electron microscopy analyses. The effects of hydrothermal reaction parameters on the adsorption properties of modified APT were intensively investigated. It was revealed that the rearrangement of crystal structure and the surface functionalization of APT with ／COOH groups cause the surprising increase of adsorption capability for Methylene Blue（MB）. The removal ratio of raw APT for MB is only 59.52%, while modified APT could almost completely remove MB in the 200 mg/L of MB solution with a removal ratio of 99.8%. The adsorption kinetics fitted pseudo second-order kinetic model, and the adsorption isotherm could be described with Langmuir isotherm model very well. The hydrogen-bonding interaction, electrostatic attraction and chemical association are the main driving force for the adsorption process.展开更多
Blue honeysuckle is rich in polyphenols, and recently receiving attention because of its potential antioxidant and anti-inflammatory properties. Nonalcoholic fatty liver disease(NAFLD) is the leading cause of chronic ...Blue honeysuckle is rich in polyphenols, and recently receiving attention because of its potential antioxidant and anti-inflammatory properties. Nonalcoholic fatty liver disease(NAFLD) is the leading cause of chronic liver disease that develops hepatic inflammation and metabolic syndrome. The present study aims to study the effect of blue honeysuckle extract(BHE) on fat deposition and hepatic lipid peroxidation in a high-fat-diet(HFD)-induced mouse model. Mice were fed a normal diet(ND) or a HFD containing 0.5% or 1% of BHE or not for 45 d. Liver sections were stained by hematoxylin-eosin staining.Serum lipids were measured by a clinical analyzer. Insulin was examined by ELISA, and hepatic proteins were detected by Western blotting. Dietary supplementation of BHE dose-dependently suppressed HFD-induced obesity and hepatic fat deposition. Moreover, BHE improved glucose metabolism by increasing insulin sensitivity and attenuated oxidative stress potentially by up-regulating nuclear factor(erythroid-derived 2)-like 2(Nrf2)-mediated pathway.展开更多
文摘AIM:To investigate whether activated carbon nanoparticles suspension(ACNS) or methylene blue(MB) can increase the detected number of lymph nodes in colorectal cancer.METHODS:Sixty-seven of 72 colorectal cancer patients treated at our hospital fulfilled the inclusion criteria of the study which was conducted from December 2010 to February 2012.Seven patients refused to participate.Eventually,60 patients were included,and randomly assigned to three groups(20 in each group):ACNS group(group A),MB group(group B) and non-stained conventional surgical group(group C).In group A,patients received subserosal injection of 1 mL ACNS in a 4-quadrant region around the mass.In group B,the main artery of specimen was identified and isolated after the specimen was removed,and 2 mL MB was slowly injected into the isolated,stretched and fixed vessel.In group C,no ACNS and MB were injected.All the mesentery lymph nodes were isolated and removed systematically by visually inspecting and palpating the adipose tissue.RESULTS:No difference was observed among the three groups in age,gender,tumor location,tumor diameter,T-stage,degree of differentiation,postoperative complications and peritoneal drainage retention time.The total number of detected lymph nodes was 535,476 and 223 in the three groups,respectively.The mean number of detected lymph nodes per patient was significantly higher in group A than in group C(26.8 ± 8.4 vs 12.2 ± 3.2,P < 0.001).Similarly,there were significantly more lymph nodes detected in group B than in group C(23.8 ± 6.9 vs 12.2 ± 3.2,P < 0.001).However,there was no significant difference between group A and group B.There were 50,46 and 32 metastatic lymph nodes dissected in 13 patients of group A,10 patients of group B and 11 patients of group C,without significant differences among the three groups.Eleven of the 60 patients had insufficient number of detected lymph nodes(< 12).Only one patient with T 4a rectal cancer had 10 lymph nodes detected in group B,the other 10 patients were all from group C.Based on
文摘The plasma membrane （PM） H^＋-ATPase is an important ion pump in the plant cell membrane. By extruding protons from the cell and generating a membrane potential, this pump energizes the PM, which is a prerequisite for growth. Modification of the autoinhibitory terminal domains activates PM H^＋-ATPase activity, and on this basis it has been hypothesized that these regulatory termini are targets for physiological factors that activate or inhibit proton pumping. In this review, we focus on the posttranslational regulation of the PM H＋-ATPase and place regulation of the pump in an evolutionary and physiological context. The emerging picture is that multiple signals regulating plant growth interfere with the posttranslational regulation of the PM H^＋-ATPase.
基金supported by the National Natural Science Foundation of China (No.20877077)the Project of Jiangsu Provincial Science and Technology Office (No.BE2008087)
文摘A series of chitosan-g-poly （acrylic acid）/vermiculite hydrogel composites were synthesized and used as adsorbents for the investigation of the effect of process parameters such as vermiculite content, pH of dye solution, contact time, initial concentration of dye solution, temperature, ionic strength and concentration of surfactant sodium dodecyl sulfate on the removal of Methylene Blue （MB） from aqueous solution. The results showed that the adsorption capacity for dye increased with increasing pH, contact time and initial dye concentration, but decreased with increasing temperature, ionic strength and sodium dodecyl sulfate concentration in the present of the surfactant. The adsorption kinetics of MB onto the hydrogel composite followed pseudo second-order kinetics and the adsorption equilibrium data obeyed Langmuir isotherm. By introducing 10 wt.% vermiculite into chitosan-g-poly （acrylic acid） polymeric network, the obtaining hydrogel composite showed the highest adsorption capacity for MB, and then could be regarded as a potential adsorbent for cationic dye removal in a wastewater treatment process.
文摘Owing to the worldwide abundance and low-cost of Na, room-temperature Na-ion batteries are emerging as attractive energy storage systems for large- scale grids. Increasing the Na content in cathode materials is one of the effective ways to achieve high energy density. Prussian blue and its analogues （PBAs） are promising Na-rich cathode materials since they can theoretically store two Na＋ ions per formula unit. However, increasing the Na content in PBAs cathode materials remains a major challenge. Here we show that sodium iron hexacyanoferrate with high Na content can be obtained by simply controlling the reducing agent and reaction atmosphere during synthesis. The Na content can reach as high as 1.63 per formula, which is the highest value for sodium iron hexacyanoferrate. This Na-rich sodium iron hexacyanoferrate demonstrates a high specific capacity of 150 mAh·g^-1 and remarkable cycling performance with 90% capacity retention after 200 cycles. Furthermore, the Na intercalation/ de-intercalation mechanism has been systematically studied by in situ Raman spectroscopy, X-ray diffraction and X-ray absorption spectroscopy analysis for the first time. The Na-rich sodium iron hexacyanoferrate can function as a plenteous Na reservoir and has great potential as a cathode material for practical Na-ion batteries.
文摘AIM:To investigate the predictive value of narrowband imaging with magnifying endoscopy (NBI-ME) for identifying gastric intestinal metaplasia (GIM) in unselected patients. METHODS:We prospectively evaluated consecutive patients undergoing upper endoscopy for various indications, such as epigastric discomfort/pain, anaemia, gastro-oesophageal reflux disease, suspicion of peptic ulcer disease, or chronic liver diseases. Patients underwent NBI-ME, which was performed by three blinded, experienced endoscopists. In addition, five biopsies (2 antrum, 1 angulus, and 2 corpus) were taken and examined by two pathologists unaware of the endoscopic findings to determine the presence or absence of GIM. The correlation between light blue crest (LBC) appearance and histology was measured. Moreover, we quantified the degree of LBC appearance as less than 20% (+), 20%-80% (++) and more than 80% (+++) of an image field, and the semiquantitative evaluation of LBC appearance was correlated with IM percentage from the histological findings. RESULTS:We enrolled 100 (58 F/42 M) patients who were mainly referred for gastro-esophageal reflux disease/dyspepsia (46%), cancer screening/anaemia (34%), chronic liver disease (9%), and suspected celiac disease (6%); the remaining patients were referred for other indications. The prevalence of Helicobacter pylori (H. pylori ) infection detected from the biopsies was 31%, while 67% of the patients used proton pump inhibitors. LBCs were found in the antrum of 33 patients (33%); 20 of the cases were classified as LBC+, 9 as LBC++, and 4 as LBC+++. LBCs were found in the gastric body of 6 patients (6%), with 5 of them also having LBCs in the antrum. The correlation between the appearance of LBCs and histological GIM was good, with a sensitivity of 80% (95%CI:67-92), a specificity of 96% (95%CI:93-99), a positive predictive value of 84% (95%CI:73-96), a negative predictive value of 95% (95%CI:92-98), and an accuracy of 93% (95%CI:90-97). The NBI-ME examination overlooked GIM in 8 cases, but the GIM
文摘Group size and composition of blue sheep(Pseudois nayaur)were studied in the Helan Mountains, Ningxia Autonomous Region from November to December 2003.We scanned mountain slopes with binoculars and observed with 20-60× spotting-scope. A total of 310 herds of blue sheep and 1 336 individuals were observed during the study period. Blue sheep were frequently seen in small herds of 2 to 8 individuals, which represent 94.8% of total herds observed. Herds consisting of 9 individuals or more represented 5.2%. The largest herd we observed numbered 51 individuals. Mean group size was 4.2 individuals. Blue sheep herds can be divided into three types: male herds (composed solely of males), female herds (consisting of females with or without juveniles of both sexes), and mixed herds (including adult males, females, and subadults).Among the 310 herds, female herds were counted 150 times(48.4%), mixed herds 154 times(49.7%), and male herds 6 times(1.9%). Of 1 336 blue sheep classified by sex and age, adults, subadults and juveniles composed 64.1%,20.8%, and 15.1% respectively. The female:male ratio of adults was 1∶0.73. The ratio of adult females to juveniles was 1∶0.56, which is higher than the ratio recorded in spring (1∶0.43) or summer (1∶0.44).The results showed that the Helan Mountains State Nature Reserve has succeed in protecting blue sheep.
基金Project supported by the National Natural Science Foundation of China(No. 20677008)the Innovation Foundation of Donghua University for Doctors(No. 113-06-001900621).
文摘The novel CuO-SnO2 nanocomposite oxide photocatalysts were prepared by simple co-precipitation method, and characterized by X- ray diffraction, transmission electron microscopy, N2 adsorption-desorption measurement and UV-Vis diffuse reflectance spectroscopy. The photocatalytic activities of CuO-SnO2, evaluated using the photodegradation of Acid Blue 62 as a probe reaction under the irradiation of Xenon light, were also found to be related to the calcination temperature and the molar ratio of Cu to Sn. The maximum photocatalytic activity of the CuO-SnO2 photocatalyst was observed to be calcined at 500~C for 3 h （the molar ratio of Cu to Sn was 1：1） due to the sample with good crystallization and high surface area. It also showed much higher photocatalytic activity in treatment dye wastewater under simulated sunlight irradiation compared to Degussa P25 TiO2.
基金supported by the Hi-Tech research and Development Program (863) of China (No. 2013AA032003)the West Light Foundation of the Chinese Academy of Sciences (the Science Development Talent Teach words [2012] No. 179)
文摘Attapulgite（APT） has been frequently used for the adsorptive removal of dyes from aqueous solution owing to its unique one-dimensional nanoscale structure and low-cost, abundant,eco-friendly advantages. In this work, APT was functionalized under mild hydrothermal condition using chloroacetic acid（CA） with ／COOH functional groups to improve its adsorption properties. The effect of hydrothermal modification on the microstructure and physicochemical features of APT was investigated by Fourier transform infrared spectroscopy, X-ray diffraction and Field-emission scanning electron microscopy analyses. The effects of hydrothermal reaction parameters on the adsorption properties of modified APT were intensively investigated. It was revealed that the rearrangement of crystal structure and the surface functionalization of APT with ／COOH groups cause the surprising increase of adsorption capability for Methylene Blue（MB）. The removal ratio of raw APT for MB is only 59.52%, while modified APT could almost completely remove MB in the 200 mg/L of MB solution with a removal ratio of 99.8%. The adsorption kinetics fitted pseudo second-order kinetic model, and the adsorption isotherm could be described with Langmuir isotherm model very well. The hydrogen-bonding interaction, electrostatic attraction and chemical association are the main driving force for the adsorption process.
基金partially supported by National Natural Science Foundation of China(No.31741115)Core Research Program 1515 of Hunan Agricultural University
文摘Blue honeysuckle is rich in polyphenols, and recently receiving attention because of its potential antioxidant and anti-inflammatory properties. Nonalcoholic fatty liver disease(NAFLD) is the leading cause of chronic liver disease that develops hepatic inflammation and metabolic syndrome. The present study aims to study the effect of blue honeysuckle extract(BHE) on fat deposition and hepatic lipid peroxidation in a high-fat-diet(HFD)-induced mouse model. Mice were fed a normal diet(ND) or a HFD containing 0.5% or 1% of BHE or not for 45 d. Liver sections were stained by hematoxylin-eosin staining.Serum lipids were measured by a clinical analyzer. Insulin was examined by ELISA, and hepatic proteins were detected by Western blotting. Dietary supplementation of BHE dose-dependently suppressed HFD-induced obesity and hepatic fat deposition. Moreover, BHE improved glucose metabolism by increasing insulin sensitivity and attenuated oxidative stress potentially by up-regulating nuclear factor(erythroid-derived 2)-like 2(Nrf2)-mediated pathway.
