[Objectives]To explore the genetic mechanism that leaf length and leaf width in bitter gourd has realistic guiding significance for formulating breeding strategy.[Methods]In this paper,the cross was made between CN19-...[Objectives]To explore the genetic mechanism that leaf length and leaf width in bitter gourd has realistic guiding significance for formulating breeding strategy.[Methods]In this paper,the cross was made between CN19-1 and Thai4-6 and an F2segregation population was constructed.The genetic characteristics of leaf length and leaf width were analyzed by employing the major gene plus polygene mixed genetic model.[Results]The data analysis results demonstrated that the leaf length and leaf width of the F_(2) segregation population showed continuous distribution.The optimum model for leaf length genetic analysis was A-1,the additive effect value of the major gene was 3.1766,the dominant effect value was-3.1766,and the heritability was 74.51%.The optimum model for leaf width genetic analysis was B-1.The additive effect values of 2 pairs of major genes controlling the leaf width were 3.1917 and 0.9623,respectively.The dominant effect values were-1.3135 and-0.3463,respectively.The additive×additive interaction effect was-0.3591,the additive×dominant interaction effect was-0.2570,the dominant×additive interaction effect was 1.9916,the dominant×dominant interaction effect was 1.9686.The heritability of major genes was 96.67%.[Conclusions]This study is expected to provide a theoretical basis for the breeding of bitter gourd.展开更多
目的:研究苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用,为苦瓜桑叶复合颗粒的进一步开发应用提供数据支持。方法:SD大鼠随机分为空白对照组、高脂肥胖模型组、苦瓜桑叶复合颗粒低剂量组和高剂量组。除...目的:研究苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用,为苦瓜桑叶复合颗粒的进一步开发应用提供数据支持。方法:SD大鼠随机分为空白对照组、高脂肥胖模型组、苦瓜桑叶复合颗粒低剂量组和高剂量组。除空白对照组外,其他组大鼠均给予高脂饲料建立肥胖模型。苦瓜桑叶复合颗粒低、高剂量组每天分别灌胃0.5和1 g/kg的苦瓜桑叶复合颗粒,其他组以相同方法灌胃等体积生理盐水,连续给药8周。测定大鼠体重及血糖血脂水平,包括葡萄糖(glucose,GLU)、糖化血清蛋白(glycated serum proteins,GSP)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipid-cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipid-cholesterol,LDL-C)等。通过苏木精-伊红染色观察大鼠肝脏和结肠组织形态变化,并检测结肠紧密连接蛋白Occludin的表达。结果:苦瓜桑叶复合颗粒可有效调控高脂饮食诱导肥胖大鼠的体重和血糖血脂水平,使其恢复正常。病理切片显示苦瓜桑叶复合颗粒可降低肥胖大鼠肝脏脂滴的形成并改善结肠粘膜腺体异常形态。同时,苦瓜桑叶复合颗粒可有效提升肥胖大鼠结肠Occludin蛋白的表达(P<0.05),改善肠粘膜紧密连接状态。结论:苦瓜桑叶复合颗粒对高脂饮食诱导的肥胖大鼠脂糖代谢异常具有明显改善作用,并可一定程度修复肥胖引起的肠道粘膜损伤,此研究对苦瓜桑叶复合颗粒的开发及相关机制的阐明具有重要意义。展开更多
The purpose of this study was to evaluate the sensory property and shelf life of the processed mpedli beer using aqueous leaves extract of Vernonia amygdalina(VA)and heat treatment.The white sorghum beer was made at t...The purpose of this study was to evaluate the sensory property and shelf life of the processed mpedli beer using aqueous leaves extract of Vernonia amygdalina(VA)and heat treatment.The white sorghum beer was made at the laboratory scale using home-made procedure(Control).Following filtration,the beer was blended with an aqueous leaf extract(1/10,v/v)of VA(BUB).Pasteurization(60°C/30 min)was performed on a portion of the VA blended sample(BPB).The sensory parameters and shelf life of the three samples were evaluated at room temperature during a month storage.The sensory characteristics of blended and non-supplemented mpedli beer differed significantly(p<0.05).During storage,the colour,bitterness,aroma,odour,viscosity,texture,and overall acceptability of the processed samples were improved.Bitterness(r=0.898;p<0.01)and odour(r=0.930;p<0.01)were both highly correlated with the acceptance of the processed beer.The non-supplemented samples had the highest sensory scores 48 hours after preparation,while the relevant sensory ratings in processed BUB and BPB samples were recorded from the 12th to the 21st and 27th day of storage,respectively.The findings suggest that combining pasteurization with addition of aqueous leaf extract of VA may help small-scale brewers for improving the sensory quality and extending the shelf life of mpedli.According to the findings,the use of bitter leaf could be proposed as an alternative hop in the local brewing industry and may increase incomes of producers of local sorghum beer.展开更多
基金Hainan Science and Technology Project(ZDYF2020229ZDKJ2021010)Scientific Research Program of Hainan Key Laboratory of Vegetable Biology and Hainan Key Laboratory for Quality Regulation of Tropical Horticultural Crops(HNZDSYS(YY)-03)。
文摘[Objectives]To explore the genetic mechanism that leaf length and leaf width in bitter gourd has realistic guiding significance for formulating breeding strategy.[Methods]In this paper,the cross was made between CN19-1 and Thai4-6 and an F2segregation population was constructed.The genetic characteristics of leaf length and leaf width were analyzed by employing the major gene plus polygene mixed genetic model.[Results]The data analysis results demonstrated that the leaf length and leaf width of the F_(2) segregation population showed continuous distribution.The optimum model for leaf length genetic analysis was A-1,the additive effect value of the major gene was 3.1766,the dominant effect value was-3.1766,and the heritability was 74.51%.The optimum model for leaf width genetic analysis was B-1.The additive effect values of 2 pairs of major genes controlling the leaf width were 3.1917 and 0.9623,respectively.The dominant effect values were-1.3135 and-0.3463,respectively.The additive×additive interaction effect was-0.3591,the additive×dominant interaction effect was-0.2570,the dominant×additive interaction effect was 1.9916,the dominant×dominant interaction effect was 1.9686.The heritability of major genes was 96.67%.[Conclusions]This study is expected to provide a theoretical basis for the breeding of bitter gourd.
文摘目的:研究苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用,为苦瓜桑叶复合颗粒的进一步开发应用提供数据支持。方法:SD大鼠随机分为空白对照组、高脂肥胖模型组、苦瓜桑叶复合颗粒低剂量组和高剂量组。除空白对照组外,其他组大鼠均给予高脂饲料建立肥胖模型。苦瓜桑叶复合颗粒低、高剂量组每天分别灌胃0.5和1 g/kg的苦瓜桑叶复合颗粒,其他组以相同方法灌胃等体积生理盐水,连续给药8周。测定大鼠体重及血糖血脂水平,包括葡萄糖(glucose,GLU)、糖化血清蛋白(glycated serum proteins,GSP)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipid-cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipid-cholesterol,LDL-C)等。通过苏木精-伊红染色观察大鼠肝脏和结肠组织形态变化,并检测结肠紧密连接蛋白Occludin的表达。结果:苦瓜桑叶复合颗粒可有效调控高脂饮食诱导肥胖大鼠的体重和血糖血脂水平,使其恢复正常。病理切片显示苦瓜桑叶复合颗粒可降低肥胖大鼠肝脏脂滴的形成并改善结肠粘膜腺体异常形态。同时,苦瓜桑叶复合颗粒可有效提升肥胖大鼠结肠Occludin蛋白的表达(P<0.05),改善肠粘膜紧密连接状态。结论:苦瓜桑叶复合颗粒对高脂饮食诱导的肥胖大鼠脂糖代谢异常具有明显改善作用,并可一定程度修复肥胖引起的肠道粘膜损伤,此研究对苦瓜桑叶复合颗粒的开发及相关机制的阐明具有重要意义。
文摘The purpose of this study was to evaluate the sensory property and shelf life of the processed mpedli beer using aqueous leaves extract of Vernonia amygdalina(VA)and heat treatment.The white sorghum beer was made at the laboratory scale using home-made procedure(Control).Following filtration,the beer was blended with an aqueous leaf extract(1/10,v/v)of VA(BUB).Pasteurization(60°C/30 min)was performed on a portion of the VA blended sample(BPB).The sensory parameters and shelf life of the three samples were evaluated at room temperature during a month storage.The sensory characteristics of blended and non-supplemented mpedli beer differed significantly(p<0.05).During storage,the colour,bitterness,aroma,odour,viscosity,texture,and overall acceptability of the processed samples were improved.Bitterness(r=0.898;p<0.01)and odour(r=0.930;p<0.01)were both highly correlated with the acceptance of the processed beer.The non-supplemented samples had the highest sensory scores 48 hours after preparation,while the relevant sensory ratings in processed BUB and BPB samples were recorded from the 12th to the 21st and 27th day of storage,respectively.The findings suggest that combining pasteurization with addition of aqueous leaf extract of VA may help small-scale brewers for improving the sensory quality and extending the shelf life of mpedli.According to the findings,the use of bitter leaf could be proposed as an alternative hop in the local brewing industry and may increase incomes of producers of local sorghum beer.
