In recent years,a new wave of bioactive,biocompatibility and biodegradable metallic materials were developed for orthopedic applications.Pure Magnesium,Magnesium alloys,Magnesium alloy-based composites are extensive m...In recent years,a new wave of bioactive,biocompatibility and biodegradable metallic materials were developed for orthopedic applications.Pure Magnesium,Magnesium alloys,Magnesium alloy-based composites are extensive material to the biomedical applications,by virtue of its high biocompatibility and reasonable strength.Pure magnesium,Magnesium alloys can corrode too fast during the physiological conditions and loses their properties before bone heal.The new era for the development of magnesium-based composites can satisfy the orthopedic applications.Magnesium-based composites,as bio-materials,can produce adjustable mechanical properties like Ultimate tensile strength,ductility,elastic modulus,and corrosion resistance in the physiological conditions.In the Mg based composites,the matrix materials are biomedical magnesium alloys base like Mg-Ca,Mg-Al,Mg-Zn,and Mg-REE alloy and The reinforcements are based on hydroxyapatite(HAP),calcium polyphosphate(CPP),andβ-tricalcium phosphate(β-TCP)particles.This comprehensive review is focused on different grades of biodegradable magnesium matrix composites including their mechanical properties and corrosion resistance.展开更多
INTRODUCTIONDevelopment of drug-resistance to chemotherapyand subsequent metastasis of tumor are primarilyresponsible for treatment failure and the death fromcancer. There have been many previous studies onthe relatio...INTRODUCTIONDevelopment of drug-resistance to chemotherapyand subsequent metastasis of tumor are primarilyresponsible for treatment failure and the death fromcancer. There have been many previous studies onthe relationship between expression of multidrugresistance (MDR) phenotype P-glycoprotein (P-gp)and the malignant properties of tumors, but theresults are often conflicting[1-8]. The difference intumor types or MDR phenotype induced by specificagents might account for this discrepancy. Taxotere(TXT), a member of the family of taxanes, hasantitumor activity through its effect of promotingthe polymerization of tubulin[9,10].展开更多
We demonstrate a simple and efficient biosynthesis method to prepare easily harvested biocompatible cadmium telluride(CdTe)quantum dots(QDs)with tunable fluorescence emission using yeast cells.Ultraviolet-visible(UV-v...We demonstrate a simple and efficient biosynthesis method to prepare easily harvested biocompatible cadmium telluride(CdTe)quantum dots(QDs)with tunable fluorescence emission using yeast cells.Ultraviolet-visible(UV-vis)spectroscopy,photoluminescence(PL)spectroscopy,X-ray diffraction(XRD),and transmission electron microscopy(TEM)confirm that the CdTe QDs are formed via an extracellular growth and subsequent endocytosis pathway and have size-tunable optical properties with fluorescence emission from 490 to 560 nm and a cubic zinc blende structure with good crystallinity.In particular,the CdTe QDs with uniform size(2-3.6 nm)are protein-capped,which makes them highly soluble in water,and in situ bio-imaging in yeast cells indicates that the biosynthesized QDs have good biocompatibility.This work provides an economic and environmentally friendly approach to synthesize highly fluorescent biocompatible CdTe QDs for bio-imaging and bio-labeling applications.展开更多
Fabrication of biocompatible core-shell microcapsules in a controllable and scalable manner remains an important but challenging task.Here,we develop a one-step microfluidic approach for the highthroughput production ...Fabrication of biocompatible core-shell microcapsules in a controllable and scalable manner remains an important but challenging task.Here,we develop a one-step microfluidic approach for the highthroughput production of biocompatible microcapsules,which utilizes single emulsions as templates and controls the precipitation of biocompatible polymer at the water/oil interface.The facile method enables the loading of various oils in the core and the enhancement of polymer shell strength by polyelectrolyte coating.The resulting microcapsules have the advantages of controllability,scalability,biocompatibility,high encapsulation efficiency and high loading capacity.The core-shell microcapsules are ideal delivery vehicles for programmable active release and various controlled release mechanisms are demonstrated,including burst release by vigorous shaking,pH-triggered release for targeted intestinal release and sustained release of perfume over a long period of time.The utility of our technique paves the way for practical applications of core-shell microcapsules.展开更多
Objective： To develop a novel scaffolding method for the copolymers poly lactide-co-glycolide acid （PLGA） to construct a three-dimensional （3-D） scaffold and explore its biocompatibility through culturing Schwann...Objective： To develop a novel scaffolding method for the copolymers poly lactide-co-glycolide acid （PLGA） to construct a three-dimensional （3-D） scaffold and explore its biocompatibility through culturing Schwann cells （SCs） on it. Methods： The 3-D scaffolds were made by means of melt spinning, extension and weaving. The queueing discipline of the micro-channels were observed under a scanning electronic microscope （SEM）.The sizes of the micropores and the factors of porosity were also measured. Sciatic nerves were harvested from 3-day-old Sprague Dawley （SD） rats for culture of SCs. SCs were separated, purified, and then implanted on PLGA scaffolds, gelatin sponge and poly-L-lysine （PLL）-coated tissue culture poly-styrene （TCPS） were used as biomaterial and cell-supportive controls, respectively. The effect of PLGA on the adherence, proliferation and apoptosis of SCs were examined in vitro in comparison with gelatin sponge and TCPS. Results： The micro-channels arrayed in parallel manners, and the pore sizes of the channels were uniform. No significant difference was found in the activity of Schwann cells cultured on PLGA and those on TCPS （P〉0.05）, and the DNA of PLGA scaffolds was not damaged. Conclusion： The 3-D scaffolds developed in this study have excellent structure and biocompatibility, which may be taken as a novel scaffold candidate for nerve-tissue engineering.展开更多
Many phytochemicals show promise in cancer prevention and treatment, but their low aqueous solubility, poor stability, unfavorable bioavailability, and low target specificity make administering them at therapeutic dos...Many phytochemicals show promise in cancer prevention and treatment, but their low aqueous solubility, poor stability, unfavorable bioavailability, and low target specificity make administering them at therapeutic doses unrealistic. This is particularly true for(-)-epigallocatechin gallate, curcumin, quercetin, resveratrol, and genistein. There is an increasing interest in developing novel delivery strategies for these natural products. Liposomes, micelles, nanoemulsions, solid lipid nanoparticles, nanostructured lipid carriers and poly(lactide-co-glycolide) nanoparticles are biocompatible and biodegradable nanoparticles. Those nanoparticles can increase the stability and solubility of phytochemicals, exhibit a sustained release property, enhance their absorption and bioavailability, protect them from premature enzymatic degradation or metabolism, prolong their circulation time, improve their target specificity to cancer cells or tumors via passive or targeted delivery, lower toxicity or side-effects to normal cells or tissues through preventing them from prematurely interacting with the biological environment, and enhance anti-cancer activities. Nanotechnology opens a door for developing phytochemical-loaded nanoparticles for prevention and treatment of cancer.展开更多
Biocompatible devices are widely employed in modernized lives and medical fields in the forms of wearable and implantable devices,raising higher requirements on the battery biocompatibility,high safety,low cost,and ex...Biocompatible devices are widely employed in modernized lives and medical fields in the forms of wearable and implantable devices,raising higher requirements on the battery biocompatibility,high safety,low cost,and excellent electrochemical performance,which become the evaluation criteria toward developing feasible biocompatible batteries.Herein,through conducting the battery implantation tests and leakage scene simulations on New Zealand rabbits,zinc sulfate electrolyte is proved to exhibit higher biosecurity and turns out to be one of the ideal zinc salts for biocompatible zinc-ion batteries(ZIBs).Furthermore,in order to mitigate the notorious dendrite growth and hydrogen evolution in mildly acidic electrolyte as well as improve their operating stability,Sn hetero nucleus is introduced to stabilize the zinc anode,which not only facilitates the planar zinc deposition,but also contributes to higher hydrogen evolution overpotential.Finally,a long lifetime of 1500 h for the symmetrical cell,the specific capacity of 150 mAh g^(-1)under 0.5 A g^(-1)for the Zn-MnO_(2)battery and 212 mAh g^(-1)under 5 A g^(-1)for the Zn—NH4V4O10 battery are obtained.This work may provide unique perspectives on biocompatible ZIBs toward the biosecurity of their cell components.展开更多
Objective To review the current crosslinking strategies for acelluar matrix scaffold,laying the foundation for subsequent experiment.Data sources Data were mainly obtained from recent papers published in PubMed or ind...Objective To review the current crosslinking strategies for acelluar matrix scaffold,laying the foundation for subsequent experiment.Data sources Data were mainly obtained from recent papers published in PubMed or indexed by Web of Science,with keyword like crosslinking.Results Various crosslinking strategies,including chemical,physical and biological methods,have been introduced to facilitate the performance of fresh acellular matrix.Chemical crosslinking reagents,involved in synthetic and naturally derived agents,need to be eliminated before implantation in case of their potential biotoxicity,although several crosslinking agents with less toxicity and specific characteristics have been developed.Physical crosslinking methods present to be safe,additive-free and relatively controllable for rapid surface functionalization with no consideration of remaining radioactivity.Biological crosslinking strategies have attracted great interest,and have been demonstrated to enhance collagen-based crosslinking since their preparations do not need toxic or potentially biologically contaminated substances and can be carried out under physiological conditions.Conclusions Kinds of crosslinking methods with its potential advantages have been developed to modify raw acelluar matrix,of which the performance are promising after being crosslinked by several crosslinking treatments.Further preclinical and clinical evaluations should be taken to vertify their safety and efficacy for the tissues and organs substitutes in tissue and regenerative medicine.展开更多
Nanozyme antibacterial agents with high enzyme-like catalytic activity and strong bacteria-binding ability have provided an alternative method to efficiently disinfect drug-resistance microorganism.Herein,the carbon n...Nanozyme antibacterial agents with high enzyme-like catalytic activity and strong bacteria-binding ability have provided an alternative method to efficiently disinfect drug-resistance microorganism.Herein,the carbon nitride quantum dots(CNQDs)nanozymes with high nitrogen vacancies(NVs)were mass-productively prepared by a simple ultrasonic-crushing method assisted by propylene glycol.It was found that the NVs of CNQDs were stemmed from the selective breaking of surface N-(C)_(2)sites,accounting for 6.2%.Experiments and density functional theory(DFT)simulations have demonstrated that the presence of NVs can alter the local electron distribution and extend theπ-electron delocalization to enhance the peroxidase-like activity.Biocompatible CNQDs could enter inside microorganisms by diffusion and elevate the bacteria-binding ability,which enhanced the accurate and rapid attack of·OH to the microorganisms.The sterilization rate of CNQDs against Gram-negative bacteria(E.coli),Gram-positive bacteria(S.aureus,B.subtilis),fungi(R.solani)reaches more than 99%.Thus,this work showed great potential for engineered nanozymes for broad-spectrum antibacterial in biomedicine and environmental protection.展开更多
Bone regeneration is a critical area in regenerative medicine,particularly in orthopedics,demanding effective biomedical materials for treating bone defects.45S5 bioactive glass(45S5 BG)is a promising material because...Bone regeneration is a critical area in regenerative medicine,particularly in orthopedics,demanding effective biomedical materials for treating bone defects.45S5 bioactive glass(45S5 BG)is a promising material because of its osteoconductive and bioactive properties.As research in this field continues to advance,keeping up-to-date on the latest and most successful applications of this material is imperative.To achieve this,we conducted a comprehensive search on Pub-Med/MEDLINE,focusing on English articles published in the last decade.Our search used the keywords“bioglass 45S5 AND bone defect”in combination.We found 27 articles,and after applying the inclusion criteria,we selected 15 studies for detailed examination.Most of these studies compared 45S5 BG with other cement or scaffold materials.These comparisons demonstrate that the addition of various composites enhances cellular biocompatibility,as evidenced by the cells and their osteogenic potential.Moreover,the use of 45S5 BG is enhanced by its antimicrobial properties,opening avenues for additional investigations and applications of this biomaterial.展开更多
文摘In recent years,a new wave of bioactive,biocompatibility and biodegradable metallic materials were developed for orthopedic applications.Pure Magnesium,Magnesium alloys,Magnesium alloy-based composites are extensive material to the biomedical applications,by virtue of its high biocompatibility and reasonable strength.Pure magnesium,Magnesium alloys can corrode too fast during the physiological conditions and loses their properties before bone heal.The new era for the development of magnesium-based composites can satisfy the orthopedic applications.Magnesium-based composites,as bio-materials,can produce adjustable mechanical properties like Ultimate tensile strength,ductility,elastic modulus,and corrosion resistance in the physiological conditions.In the Mg based composites,the matrix materials are biomedical magnesium alloys base like Mg-Ca,Mg-Al,Mg-Zn,and Mg-REE alloy and The reinforcements are based on hydroxyapatite(HAP),calcium polyphosphate(CPP),andβ-tricalcium phosphate(β-TCP)particles.This comprehensive review is focused on different grades of biodegradable magnesium matrix composites including their mechanical properties and corrosion resistance.
基金Supported in part by phone-Poulenc Rorer Pharmaceuticals INC
文摘INTRODUCTIONDevelopment of drug-resistance to chemotherapyand subsequent metastasis of tumor are primarilyresponsible for treatment failure and the death fromcancer. There have been many previous studies onthe relationship between expression of multidrugresistance (MDR) phenotype P-glycoprotein (P-gp)and the malignant properties of tumors, but theresults are often conflicting[1-8]. The difference intumor types or MDR phenotype induced by specificagents might account for this discrepancy. Taxotere(TXT), a member of the family of taxanes, hasantitumor activity through its effect of promotingthe polymerization of tubulin[9,10].
基金This work was supported by Discovery Grants from the Australian Research Council(No.DP0879769).
文摘We demonstrate a simple and efficient biosynthesis method to prepare easily harvested biocompatible cadmium telluride(CdTe)quantum dots(QDs)with tunable fluorescence emission using yeast cells.Ultraviolet-visible(UV-vis)spectroscopy,photoluminescence(PL)spectroscopy,X-ray diffraction(XRD),and transmission electron microscopy(TEM)confirm that the CdTe QDs are formed via an extracellular growth and subsequent endocytosis pathway and have size-tunable optical properties with fluorescence emission from 490 to 560 nm and a cubic zinc blende structure with good crystallinity.In particular,the CdTe QDs with uniform size(2-3.6 nm)are protein-capped,which makes them highly soluble in water,and in situ bio-imaging in yeast cells indicates that the biosynthesized QDs have good biocompatibility.This work provides an economic and environmentally friendly approach to synthesize highly fluorescent biocompatible CdTe QDs for bio-imaging and bio-labeling applications.
基金supported by the National Natural Science Foundation of China (Nos.21878258 and 11704331)"theFundamental Research Funds for the Central Universities" (No. 2018QNA4046)+2 种基金the Youth Funds of the State Key Laboratory of Fluid Power and Mechatronic Systems (Zhejiang University)supported by the National Science Foundation (No. DMR-1310266)the Harvard Materials Research Science and Engineering Center (No.DMR-1420570)
文摘Fabrication of biocompatible core-shell microcapsules in a controllable and scalable manner remains an important but challenging task.Here,we develop a one-step microfluidic approach for the highthroughput production of biocompatible microcapsules,which utilizes single emulsions as templates and controls the precipitation of biocompatible polymer at the water/oil interface.The facile method enables the loading of various oils in the core and the enhancement of polymer shell strength by polyelectrolyte coating.The resulting microcapsules have the advantages of controllability,scalability,biocompatibility,high encapsulation efficiency and high loading capacity.The core-shell microcapsules are ideal delivery vehicles for programmable active release and various controlled release mechanisms are demonstrated,including burst release by vigorous shaking,pH-triggered release for targeted intestinal release and sustained release of perfume over a long period of time.The utility of our technique paves the way for practical applications of core-shell microcapsules.
文摘Objective： To develop a novel scaffolding method for the copolymers poly lactide-co-glycolide acid （PLGA） to construct a three-dimensional （3-D） scaffold and explore its biocompatibility through culturing Schwann cells （SCs） on it. Methods： The 3-D scaffolds were made by means of melt spinning, extension and weaving. The queueing discipline of the micro-channels were observed under a scanning electronic microscope （SEM）.The sizes of the micropores and the factors of porosity were also measured. Sciatic nerves were harvested from 3-day-old Sprague Dawley （SD） rats for culture of SCs. SCs were separated, purified, and then implanted on PLGA scaffolds, gelatin sponge and poly-L-lysine （PLL）-coated tissue culture poly-styrene （TCPS） were used as biomaterial and cell-supportive controls, respectively. The effect of PLGA on the adherence, proliferation and apoptosis of SCs were examined in vitro in comparison with gelatin sponge and TCPS. Results： The micro-channels arrayed in parallel manners, and the pore sizes of the channels were uniform. No significant difference was found in the activity of Schwann cells cultured on PLGA and those on TCPS （P〉0.05）, and the DNA of PLGA scaffolds was not damaged. Conclusion： The 3-D scaffolds developed in this study have excellent structure and biocompatibility, which may be taken as a novel scaffold candidate for nerve-tissue engineering.
基金supported by grant from the National Center for Complementary&Integrative Health(Nos.R15AT007013 and R15AT008733)
文摘Many phytochemicals show promise in cancer prevention and treatment, but their low aqueous solubility, poor stability, unfavorable bioavailability, and low target specificity make administering them at therapeutic doses unrealistic. This is particularly true for(-)-epigallocatechin gallate, curcumin, quercetin, resveratrol, and genistein. There is an increasing interest in developing novel delivery strategies for these natural products. Liposomes, micelles, nanoemulsions, solid lipid nanoparticles, nanostructured lipid carriers and poly(lactide-co-glycolide) nanoparticles are biocompatible and biodegradable nanoparticles. Those nanoparticles can increase the stability and solubility of phytochemicals, exhibit a sustained release property, enhance their absorption and bioavailability, protect them from premature enzymatic degradation or metabolism, prolong their circulation time, improve their target specificity to cancer cells or tumors via passive or targeted delivery, lower toxicity or side-effects to normal cells or tissues through preventing them from prematurely interacting with the biological environment, and enhance anti-cancer activities. Nanotechnology opens a door for developing phytochemical-loaded nanoparticles for prevention and treatment of cancer.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.82103472,82202480,and 52372252)the Hunan Natural Science Fund for Distinguished Young Scholar(2021JJ10064)the Program of Youth Talent Support for Hunan Province(2020RC3011).
文摘Biocompatible devices are widely employed in modernized lives and medical fields in the forms of wearable and implantable devices,raising higher requirements on the battery biocompatibility,high safety,low cost,and excellent electrochemical performance,which become the evaluation criteria toward developing feasible biocompatible batteries.Herein,through conducting the battery implantation tests and leakage scene simulations on New Zealand rabbits,zinc sulfate electrolyte is proved to exhibit higher biosecurity and turns out to be one of the ideal zinc salts for biocompatible zinc-ion batteries(ZIBs).Furthermore,in order to mitigate the notorious dendrite growth and hydrogen evolution in mildly acidic electrolyte as well as improve their operating stability,Sn hetero nucleus is introduced to stabilize the zinc anode,which not only facilitates the planar zinc deposition,but also contributes to higher hydrogen evolution overpotential.Finally,a long lifetime of 1500 h for the symmetrical cell,the specific capacity of 150 mAh g^(-1)under 0.5 A g^(-1)for the Zn-MnO_(2)battery and 212 mAh g^(-1)under 5 A g^(-1)for the Zn—NH4V4O10 battery are obtained.This work may provide unique perspectives on biocompatible ZIBs toward the biosecurity of their cell components.
基金This work was supported by a grant from the National Natural Science Foundation of China （No. 81371702）.
文摘Objective To review the current crosslinking strategies for acelluar matrix scaffold,laying the foundation for subsequent experiment.Data sources Data were mainly obtained from recent papers published in PubMed or indexed by Web of Science,with keyword like crosslinking.Results Various crosslinking strategies,including chemical,physical and biological methods,have been introduced to facilitate the performance of fresh acellular matrix.Chemical crosslinking reagents,involved in synthetic and naturally derived agents,need to be eliminated before implantation in case of their potential biotoxicity,although several crosslinking agents with less toxicity and specific characteristics have been developed.Physical crosslinking methods present to be safe,additive-free and relatively controllable for rapid surface functionalization with no consideration of remaining radioactivity.Biological crosslinking strategies have attracted great interest,and have been demonstrated to enhance collagen-based crosslinking since their preparations do not need toxic or potentially biologically contaminated substances and can be carried out under physiological conditions.Conclusions Kinds of crosslinking methods with its potential advantages have been developed to modify raw acelluar matrix,of which the performance are promising after being crosslinked by several crosslinking treatments.Further preclinical and clinical evaluations should be taken to vertify their safety and efficacy for the tissues and organs substitutes in tissue and regenerative medicine.
基金the National Natural Science Foundation of China(Nos.21876099,22106088,and 22276110)Key Research&Developmental Program of Shandong Province(No.2021CXGC011202)Fundamental Research Funds of Shandong University(No.zy202102).
文摘Nanozyme antibacterial agents with high enzyme-like catalytic activity and strong bacteria-binding ability have provided an alternative method to efficiently disinfect drug-resistance microorganism.Herein,the carbon nitride quantum dots(CNQDs)nanozymes with high nitrogen vacancies(NVs)were mass-productively prepared by a simple ultrasonic-crushing method assisted by propylene glycol.It was found that the NVs of CNQDs were stemmed from the selective breaking of surface N-(C)_(2)sites,accounting for 6.2%.Experiments and density functional theory(DFT)simulations have demonstrated that the presence of NVs can alter the local electron distribution and extend theπ-electron delocalization to enhance the peroxidase-like activity.Biocompatible CNQDs could enter inside microorganisms by diffusion and elevate the bacteria-binding ability,which enhanced the accurate and rapid attack of·OH to the microorganisms.The sterilization rate of CNQDs against Gram-negative bacteria(E.coli),Gram-positive bacteria(S.aureus,B.subtilis),fungi(R.solani)reaches more than 99%.Thus,this work showed great potential for engineered nanozymes for broad-spectrum antibacterial in biomedicine and environmental protection.
文摘Bone regeneration is a critical area in regenerative medicine,particularly in orthopedics,demanding effective biomedical materials for treating bone defects.45S5 bioactive glass(45S5 BG)is a promising material because of its osteoconductive and bioactive properties.As research in this field continues to advance,keeping up-to-date on the latest and most successful applications of this material is imperative.To achieve this,we conducted a comprehensive search on Pub-Med/MEDLINE,focusing on English articles published in the last decade.Our search used the keywords“bioglass 45S5 AND bone defect”in combination.We found 27 articles,and after applying the inclusion criteria,we selected 15 studies for detailed examination.Most of these studies compared 45S5 BG with other cement or scaffold materials.These comparisons demonstrate that the addition of various composites enhances cellular biocompatibility,as evidenced by the cells and their osteogenic potential.Moreover,the use of 45S5 BG is enhanced by its antimicrobial properties,opening avenues for additional investigations and applications of this biomaterial.
