Para-amino benzoic acid(PABA),a folic acid related metabolite,was first introduced to fabricate micro-grooves and improve hydrophilicity over surfaces of carbon fibers(CFs).Then,engineered CFs/poly(lactic acid)-poly(e...Para-amino benzoic acid(PABA),a folic acid related metabolite,was first introduced to fabricate micro-grooves and improve hydrophilicity over surfaces of carbon fibers(CFs).Then,engineered CFs/poly(lactic acid)-poly(ethylene glycol)(PLA-PEG) biocomposites were fabricated by a solvent casting/particulate leaching method.We found that introducing small hydrophobic PABA molecules and fabricating patterned structures would lead to benign integrated interfaces between CFs and the PLA-PEG matrix.Specifically,the compressive strength of CFs/PLA-PEG was improved from 3.98 to 5.48 MPa.In addition,the CFs/PLA-PEG biocomposites significantly accelerated the adhesion and proliferation of pre-osteoblasts with minimized cytotoxidty.By comparing the cyto-compatibility of L929 and MC_3T_3 cells cultured on different modified PLA-PEG composites,it could be concluded that PABA-CFs not only overcame the limitation of poor strength of PLA-PEG,but also improved the cell growth.These results indicate that the PABA-CFs reinforced PLA-PEG biocomposites could be a potential alternative for tissue engineering scaffolds.展开更多
基金supported by the National Key Research and Development Project(2016YFB0303201)the Research and Innovation Project of Shanghai Municipal Education Commission(14zz069)Donghua University Graduates'Innovation Funding Projects(EG2015006)
文摘Para-amino benzoic acid(PABA),a folic acid related metabolite,was first introduced to fabricate micro-grooves and improve hydrophilicity over surfaces of carbon fibers(CFs).Then,engineered CFs/poly(lactic acid)-poly(ethylene glycol)(PLA-PEG) biocomposites were fabricated by a solvent casting/particulate leaching method.We found that introducing small hydrophobic PABA molecules and fabricating patterned structures would lead to benign integrated interfaces between CFs and the PLA-PEG matrix.Specifically,the compressive strength of CFs/PLA-PEG was improved from 3.98 to 5.48 MPa.In addition,the CFs/PLA-PEG biocomposites significantly accelerated the adhesion and proliferation of pre-osteoblasts with minimized cytotoxidty.By comparing the cyto-compatibility of L929 and MC_3T_3 cells cultured on different modified PLA-PEG composites,it could be concluded that PABA-CFs not only overcame the limitation of poor strength of PLA-PEG,but also improved the cell growth.These results indicate that the PABA-CFs reinforced PLA-PEG biocomposites could be a potential alternative for tissue engineering scaffolds.
