The unique composition of milk makes this basic foodstuff into an exceptional raw material for the production of new ingredients with desired properties and diverse applications in the food industry. The fractionation...The unique composition of milk makes this basic foodstuff into an exceptional raw material for the production of new ingredients with desired properties and diverse applications in the food industry. The fractionation of milk is the key in the development of those ingredients and products;hence continuous research and development on this field, especially various levels of fractionation and separation by filtration, have been carried out. This review focuses on the production of milk fractions as well as their particular properties, applications and processes that increase their exploitation. Whey proteins and caseins from the protein fraction are excellent emulsifiers and protein supplements. Besides, they can be chemically or enzymatically modified to obtain bioactive peptides with numerous functional and nutritional properties. In this context, valorization techniques of cheese-whey proteins, by-product of dairy industry that constitutes both economic and environmental problems, are being developed. Phospholipids from the milk fat fraction are powerful emulsifiers and also have exclusive nutraceutical properties. In addition, enzyme modification of milk phospholipids makes it possible to tailor emulsifiers with particular properties. However, several aspects remain to be overcome;those refer to a deeper understanding of the healthy, functional and nutritional properties of these new ingredients that might be barriers for its use and acceptability. Additionally, in this review, alternative applications of milk constituents in the non-food area such as in the manufacture of plastic materials and textile fibers are also introduced. The unmet needs, the cross-fertilization in between various protein domains,the carbon footprint requirements, the environmental necessities, the health and wellness new demand, etc., are dominant factors in the search for innovation approaches;these factors are also outlining the further innovation potential deriving from those “apparent” constrains obliging science and technology展开更多
Natural products are great treasure troves for the discovery of bioactive components.Current bioassay guided fractionation for identification of bioactive components is time-and workload-consuming.In this study,we pro...Natural products are great treasure troves for the discovery of bioactive components.Current bioassay guided fractionation for identification of bioactive components is time-and workload-consuming.In this study,we proposed a robust and convenient strategy for deciphering the bioactive profile of natural products by mass spectral molecular networking combined with rapid bioassay.As a proof-of-concept,the strategy was applied to identify angiotensin converting enzyme(ACE)inhibitors of Fangjihuangqi decoction(FJHQD),a traditional medicine clinically used for the treatment of heart failure.The chemical profile of FJHQD was comprehensively revealed with the assistance of tandem mass spectral molecular networking,and a total of 165 compounds were identified.With characterized constituents,potential clinical applications of FJHQD were predicted by Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine,and a range of cardiovascular related diseases were significantly enriched.ACE inhibitory activities of FJHQD and its constituents were then investigated with an aggregation-induced emission based fluorescent probe.FJHQD exhibited excellent ACE inhibitory effects,and a bioactive molecular network was established to elucidate the ACE inhibitory profile of constituents in FJHQD.This bioactive molecular network provided a panoramic view of FJHQD’s ACE inhibitory activities,which demonstrated that flavones from Astragali Radix and Glycyrrhizae Radix et Rhizoma,saponins from Astragali Radix,and sesquiterpenoids from Atractylodis Macrocephalae Rhizoma were principal components responsible for this effect of FJHQD.Among them,four novel ACE inhibitors were the first to be reported.Our study indicated that the proposed strategy offers a useful approach to uncover the bioactive profile of traditional medicines and provides a pragmatic workflow for exploring bioactive components.展开更多
Two kinds of water-soluble metallophthalocyanines, binuclear cobalt phthalocyanine （Co2Pc2） and binuclear ferric phthalocyanine （Fe2Pc2）, were synthesized through phenylanhydride-urea route and characterized by el...Two kinds of water-soluble metallophthalocyanines, binuclear cobalt phthalocyanine （Co2Pc2） and binuclear ferric phthalocyanine （Fe2Pc2）, were synthesized through phenylanhydride-urea route and characterized by elemental analysis and FT-IR spectra. Binuclear metallophthalocyanine derivatives （Mt2Pc2） were immobilized on silk fibers and modified viscose fibers to construct bioactive fibers of mimic enzyme. Mt2Pc2 was used as the active center of bioactive fibers, viscose and silk fibers as the microenvironments. The catalytic oxidation ability of bioactive fibers on the malodors of methanthiol and hydrogen sulfide was investigated at room temperature. The experimental results indicated that the catalytic activity of such bioactive fibers was closely correlative to the types ofbioactive fibers and substrates.展开更多
Caralluma edulis is a leafless succulent herb native to India and Pakistan,where it is used as emergency food and as potential herbal medicine.It was investigated for its bioactive chemicals and further medicinal prop...Caralluma edulis is a leafless succulent herb native to India and Pakistan,where it is used as emergency food and as potential herbal medicine.It was investigated for its bioactive chemicals and further medicinal properties.The objective of the current study was to accomplish the chemical and biological characterization of C.edulis.Chemical profiling was done through estimation of total bioactive contents and UHPLMS analysis.Biological screening was achieved through six different antioxidant and enzyme inhibition assays.Estimation of its phenolic and flavonoid contents revealed that ethyl acetate fraction(Ce-E)contains 14.15 mg GAE/g extract and 26.13 mg RE/g extract of phenolics and flavonoids,respectively,followed by the methanolic(Ce-M)and the water(Ce-W)soluble fractions.In free radical inhibitory assays,Ce-E fraction also exhibited highest activity(DPPH:19.72 mg TE/g extract,ABTS:48.66 mg TE/g extract)followed by Ce-M,whereas,in CUPRAC and FRAP assays,all the extracts,except water fraction,exhibited nearly equal potential in the range of 53.85–57.96 and 25.42–32.12 mg TE/g extract,respectively.In metal chelating antioxidant assay,only Ce-M and Ce-W fractions displayed considerable activities(25.88 and 23.55 mg EDTAE/g extract respectively),whereas,Ce-H exhibited the highest activity in phosphomolybdenum assay(1.03 mmolTE/g extract),against BChE(6.43 mg GALAE/g extract),α-glucosidase andα-amylase(6.98 and 0.47 mmol ACAE/g,respectively)and tyrosinase(64.46 mg KAE/g extract)enzymes.Ce-M and Ce-E also exhibited significant activity against tyrosinase(59.85 and 58.40 mg KAE/g extract,respectively).Crude methanolic extract(Ce-M)was analyzed to unveil its secondary metabolic picture;the UHPLCMS analysis discloses 105 compounds as phenolic acids,flavonoids,steroids and their glycosides,which makes the C.edulis extract a strong candidate for nutraceutical and functional food ingredient.Some of the metabolites were docked against all the tested enzymes to predict mode of actions and to substantiate the pharmaceu展开更多
文摘The unique composition of milk makes this basic foodstuff into an exceptional raw material for the production of new ingredients with desired properties and diverse applications in the food industry. The fractionation of milk is the key in the development of those ingredients and products;hence continuous research and development on this field, especially various levels of fractionation and separation by filtration, have been carried out. This review focuses on the production of milk fractions as well as their particular properties, applications and processes that increase their exploitation. Whey proteins and caseins from the protein fraction are excellent emulsifiers and protein supplements. Besides, they can be chemically or enzymatically modified to obtain bioactive peptides with numerous functional and nutritional properties. In this context, valorization techniques of cheese-whey proteins, by-product of dairy industry that constitutes both economic and environmental problems, are being developed. Phospholipids from the milk fat fraction are powerful emulsifiers and also have exclusive nutraceutical properties. In addition, enzyme modification of milk phospholipids makes it possible to tailor emulsifiers with particular properties. However, several aspects remain to be overcome;those refer to a deeper understanding of the healthy, functional and nutritional properties of these new ingredients that might be barriers for its use and acceptability. Additionally, in this review, alternative applications of milk constituents in the non-food area such as in the manufacture of plastic materials and textile fibers are also introduced. The unmet needs, the cross-fertilization in between various protein domains,the carbon footprint requirements, the environmental necessities, the health and wellness new demand, etc., are dominant factors in the search for innovation approaches;these factors are also outlining the further innovation potential deriving from those “apparent” constrains obliging science and technology
基金financially supported by the National Key R&D Program of China(Grant No.:2018YFC1704502)the National Natural Science Foundation of China(Grant No.:81603268)the National Natural Science Foundation of China(Grant No.:81822047)
文摘Natural products are great treasure troves for the discovery of bioactive components.Current bioassay guided fractionation for identification of bioactive components is time-and workload-consuming.In this study,we proposed a robust and convenient strategy for deciphering the bioactive profile of natural products by mass spectral molecular networking combined with rapid bioassay.As a proof-of-concept,the strategy was applied to identify angiotensin converting enzyme(ACE)inhibitors of Fangjihuangqi decoction(FJHQD),a traditional medicine clinically used for the treatment of heart failure.The chemical profile of FJHQD was comprehensively revealed with the assistance of tandem mass spectral molecular networking,and a total of 165 compounds were identified.With characterized constituents,potential clinical applications of FJHQD were predicted by Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine,and a range of cardiovascular related diseases were significantly enriched.ACE inhibitory activities of FJHQD and its constituents were then investigated with an aggregation-induced emission based fluorescent probe.FJHQD exhibited excellent ACE inhibitory effects,and a bioactive molecular network was established to elucidate the ACE inhibitory profile of constituents in FJHQD.This bioactive molecular network provided a panoramic view of FJHQD’s ACE inhibitory activities,which demonstrated that flavones from Astragali Radix and Glycyrrhizae Radix et Rhizoma,saponins from Astragali Radix,and sesquiterpenoids from Atractylodis Macrocephalae Rhizoma were principal components responsible for this effect of FJHQD.Among them,four novel ACE inhibitors were the first to be reported.Our study indicated that the proposed strategy offers a useful approach to uncover the bioactive profile of traditional medicines and provides a pragmatic workflow for exploring bioactive components.
基金This work was supported by the National Natural Science Foundation of China (No. 50373038)Program for New Century Excellent Talents in Universities (No. NCET-04-0559)Key International Cooperation of Science and Technology of Zhejiang (No. 2005C14013).
文摘Two kinds of water-soluble metallophthalocyanines, binuclear cobalt phthalocyanine （Co2Pc2） and binuclear ferric phthalocyanine （Fe2Pc2）, were synthesized through phenylanhydride-urea route and characterized by elemental analysis and FT-IR spectra. Binuclear metallophthalocyanine derivatives （Mt2Pc2） were immobilized on silk fibers and modified viscose fibers to construct bioactive fibers of mimic enzyme. Mt2Pc2 was used as the active center of bioactive fibers, viscose and silk fibers as the microenvironments. The catalytic oxidation ability of bioactive fibers on the malodors of methanthiol and hydrogen sulfide was investigated at room temperature. The experimental results indicated that the catalytic activity of such bioactive fibers was closely correlative to the types ofbioactive fibers and substrates.
基金King Khalid University of Saudi Arabia extend their appreciation to Deanship of Scientific Research for financial support through project RGP.1/318/43support from Poison Control and Medical Forensic Chemistry Centre,Ministry of Health,Asir Region,Saudi Arabia.
文摘Caralluma edulis is a leafless succulent herb native to India and Pakistan,where it is used as emergency food and as potential herbal medicine.It was investigated for its bioactive chemicals and further medicinal properties.The objective of the current study was to accomplish the chemical and biological characterization of C.edulis.Chemical profiling was done through estimation of total bioactive contents and UHPLMS analysis.Biological screening was achieved through six different antioxidant and enzyme inhibition assays.Estimation of its phenolic and flavonoid contents revealed that ethyl acetate fraction(Ce-E)contains 14.15 mg GAE/g extract and 26.13 mg RE/g extract of phenolics and flavonoids,respectively,followed by the methanolic(Ce-M)and the water(Ce-W)soluble fractions.In free radical inhibitory assays,Ce-E fraction also exhibited highest activity(DPPH:19.72 mg TE/g extract,ABTS:48.66 mg TE/g extract)followed by Ce-M,whereas,in CUPRAC and FRAP assays,all the extracts,except water fraction,exhibited nearly equal potential in the range of 53.85–57.96 and 25.42–32.12 mg TE/g extract,respectively.In metal chelating antioxidant assay,only Ce-M and Ce-W fractions displayed considerable activities(25.88 and 23.55 mg EDTAE/g extract respectively),whereas,Ce-H exhibited the highest activity in phosphomolybdenum assay(1.03 mmolTE/g extract),against BChE(6.43 mg GALAE/g extract),α-glucosidase andα-amylase(6.98 and 0.47 mmol ACAE/g,respectively)and tyrosinase(64.46 mg KAE/g extract)enzymes.Ce-M and Ce-E also exhibited significant activity against tyrosinase(59.85 and 58.40 mg KAE/g extract,respectively).Crude methanolic extract(Ce-M)was analyzed to unveil its secondary metabolic picture;the UHPLCMS analysis discloses 105 compounds as phenolic acids,flavonoids,steroids and their glycosides,which makes the C.edulis extract a strong candidate for nutraceutical and functional food ingredient.Some of the metabolites were docked against all the tested enzymes to predict mode of actions and to substantiate the pharmaceu
