筛选高效脱硫菌株并揭示其去除硫化物的机理对生物脱硫技术的发展具有重要意义.从天然气净化厂污水站废水中分离出一株化能自养型硫氧化细菌,命名为DS-B.根据16S r DNA基因序列相似性分析,结合形态特征,对该菌株进行鉴定.进一步研究DS-...筛选高效脱硫菌株并揭示其去除硫化物的机理对生物脱硫技术的发展具有重要意义.从天然气净化厂污水站废水中分离出一株化能自养型硫氧化细菌,命名为DS-B.根据16S r DNA基因序列相似性分析,结合形态特征,对该菌株进行鉴定.进一步研究DS-B的硫代硫酸盐耐受性,并通过测定培养体系中各价态含硫物质的含量,探讨该菌的硫氧化性能.结果显示,菌株DS-B与Thioalkalivibrio thiocyanoxidans ARh2的序列相似性最高,初步鉴定其属于Thioalkalivibrio.DS-B的最佳生长和降解温度为35℃,35℃下培养7 d,硫代硫酸盐(S2O32-)的去除率能达到98.7%.该菌对S2O32-有较强的耐受性,且最适S2O32-浓度为2×104 mg/L.此外,对各价态硫氧化物的分析表明该菌氧化S2O32-的途径为:S2O32-→SO42-/S2O32-→S→SO42-.上述表明,菌株DS-B具有较强的耐盐能力,对硫代硫酸盐具有较高的去除能力,是一株具有应用价值的硫氧化细菌.展开更多
Bio-desulfurization of coal by rhodococcus sp.was studied.Some kinds of coal were swelled with different organic solvents,and then the swelled coals were treated by rhodococcus sp.The results show that the ratios of d...Bio-desulfurization of coal by rhodococcus sp.was studied.Some kinds of coal were swelled with different organic solvents,and then the swelled coals were treated by rhodococcus sp.The results show that the ratios of desulfurization of coals increase after they are swelled,especially swelled with NMP,the ratio is more than 80%.On the other side,the swelling and extraction of coal were also studied after the coal had been treated by rhodococcus sp.The results show that the ratios of swelling increase more than 65%,but the extraction yield decreases for the coal treated by rhodococcus sp.展开更多
A facultative thermophilic alkaline desulphuricant strain named GDJ-3 was isolated from neutral soils,enriched on sulfur synthetic medium,and detected in previous work.Conventional and chemotax-onomic analyses and 16s...A facultative thermophilic alkaline desulphuricant strain named GDJ-3 was isolated from neutral soils,enriched on sulfur synthetic medium,and detected in previous work.Conventional and chemotax-onomic analyses and 16s RNA gene sequencing showed that the strain was in good agreement with Alpha proteobacterium sp.(97%) and ochrobactrum sp.(98%).The regenerative processes of the solu-tion containing sulfur compounds(SCS) from the strain through an orthogonal test were investigated to get the optimum regenerative condition.The results showed that regenerative temperature,air flow,and stirring speed of the agitator were the main three variables influencing the regenerative processes of the SCS.The optimum regenerative efficiency of the SCS from the strain was obtained when tem-perature,air flow,and stirring speed of the agitator were 318.2 K,3.0 L/min,and zero r/min,respectively.Under this condition,when the cell concentration of the strain was adjusted to 107/mL,the concentra-tions of Na2S2O3 and Na2S in the SCS decreased from 112.68 g/L to 96.88 g/L and from 0.87 g/L to 0.11 g/L in 9.5 h.Meanwhile,XRD spectrum shows that sulfur was formed in the regeneration process.These results suggest that the strain has potential application to the regeneration of the industrial so-lution containing sulfur compounds.展开更多
文摘Bio-desulfurization of coal by rhodococcus sp.was studied.Some kinds of coal were swelled with different organic solvents,and then the swelled coals were treated by rhodococcus sp.The results show that the ratios of desulfurization of coals increase after they are swelled,especially swelled with NMP,the ratio is more than 80%.On the other side,the swelling and extraction of coal were also studied after the coal had been treated by rhodococcus sp.The results show that the ratios of swelling increase more than 65%,but the extraction yield decreases for the coal treated by rhodococcus sp.
基金Supported by Yongfeng Boyuan Industry Co., Ltd., Jiangxi Province, China
文摘A facultative thermophilic alkaline desulphuricant strain named GDJ-3 was isolated from neutral soils,enriched on sulfur synthetic medium,and detected in previous work.Conventional and chemotax-onomic analyses and 16s RNA gene sequencing showed that the strain was in good agreement with Alpha proteobacterium sp.(97%) and ochrobactrum sp.(98%).The regenerative processes of the solu-tion containing sulfur compounds(SCS) from the strain through an orthogonal test were investigated to get the optimum regenerative condition.The results showed that regenerative temperature,air flow,and stirring speed of the agitator were the main three variables influencing the regenerative processes of the SCS.The optimum regenerative efficiency of the SCS from the strain was obtained when tem-perature,air flow,and stirring speed of the agitator were 318.2 K,3.0 L/min,and zero r/min,respectively.Under this condition,when the cell concentration of the strain was adjusted to 107/mL,the concentra-tions of Na2S2O3 and Na2S in the SCS decreased from 112.68 g/L to 96.88 g/L and from 0.87 g/L to 0.11 g/L in 9.5 h.Meanwhile,XRD spectrum shows that sulfur was formed in the regeneration process.These results suggest that the strain has potential application to the regeneration of the industrial so-lution containing sulfur compounds.
