Critical bone defects caused by extensive excision of malignant bone tumor and the probability of tumor recurrence due to residual tumor cells make malignant bone tumor treatment a major clinical challenge.The present...Critical bone defects caused by extensive excision of malignant bone tumor and the probability of tumor recurrence due to residual tumor cells make malignant bone tumor treatment a major clinical challenge.The present therapeutic strategy concentrates on implanting bone substitutes for defect filling but suffers from failures in both enhancing bone regeneration and inhibiting the growth of tumor cells.Herein,Cu and Mn-doped borosilicate nanoparticles(BSNs)were developed for syncretic bone repairing and anti-tumor treatment,which can enhance bone regeneration through the osteogenic effects of Cu^(2+) and Mn^(3+) ions and meanwhile induce tumor cells apoptosis through the hydroxyl radicals produced by the Fenton-like reactions of Cu^(2+) and Mn^(3+) ions.In vitro study showed that both osteogenic differentiation of BMSCs and angiogenesis of endothelial cells were promoted by BSNs,and consistently the critical bone defects of rats were efficiently repaired by BSNs through in vivo evaluation.Meanwhile,BSNs could generate hydroxyl radicals through Fenton-like reactions in the simulated tumor microenvironment,promote the generation of intracellular reactive oxygen species,and eventually induce tumor cell apoptosis.Besides,subcutaneous tumors of mice were effectively inhibited by BSNs without causing toxic side effects to normal tissues and organs.Altogether,Cu and Mn-doped BSNs developed in this work performed dual functions of enhancing osteogenesis and angiogenesis for bone regeneration,and inhibiting tumor growth for chemodynamic therapy,thus holding a great potential for syncretic bone repairing and anti-tumor therapy.展开更多
A new method of preparation of biomaterial composite coating by the techniqueof subsonic thermal spraying was discussed in this paper. Ti_6Al_4V and pure Ti were chosen assubstrate and sublayer material respectively a...A new method of preparation of biomaterial composite coating by the techniqueof subsonic thermal spraying was discussed in this paper. Ti_6Al_4V and pure Ti were chosen assubstrate and sublayer material respectively and the working layer was sprayed with biomaterialhydroxyapatite (HAP), forming the composite coating. The experiments of heat shock and tensilestrength showed that the bonding strength between coating and substrate is almost as same as that ofspecimen in which Ni/Al powder was adopted as sublayer. The phases of TiN, TiO_2, and Ti_2O_3 wereformed in the sublayer, which are free of toxic and have no side effects. The powder of workinglayer HAP was decomposed partly during spraying, but it can be solved by later treatment.展开更多
Hydroxyapatite (HA) bio-ceramics implant tooth was tested through animal experiment and clinical application. HA tooth was placed into the fresh extraction socket. At first, HA tooth was chosen and implanted as fixed ...Hydroxyapatite (HA) bio-ceramics implant tooth was tested through animal experiment and clinical application. HA tooth was placed into the fresh extraction socket. At first, HA tooth was chosen and implanted as fixed maxillary front tooth in domestic rabbit immediately after extraction of tooth. Three to six months later satisfactory osseointegration between implanted HA teeth and periodontinum was observed. Clinically, 28 teeth in 25 patients were implanted with suitable HA teeth, chosen according to dental X-ray and models of different kinds of HA dental root. Crowning prosthesis with visible lightsolidfied material made the HA tooth wholesome and nice looking. Implantation and fixation of the HA teeth into the fresh extraction sockets of all the patients were successful.展开更多
基金funded by National Key Research and Development Program of China(Grant No.2018YFC1106302 and 2018YFA0703100)National Natural Science Foundation of China(Grant No.51772210,U2001221,51802340,81871774 and 82072422)+1 种基金Frontier Science Key Research Programs of CAS(Grant No.QYZDB-SSW-JSC030)Shenzhen Fundamental Research Foundation(Grant No.JCYJ20200109114620793).
文摘Critical bone defects caused by extensive excision of malignant bone tumor and the probability of tumor recurrence due to residual tumor cells make malignant bone tumor treatment a major clinical challenge.The present therapeutic strategy concentrates on implanting bone substitutes for defect filling but suffers from failures in both enhancing bone regeneration and inhibiting the growth of tumor cells.Herein,Cu and Mn-doped borosilicate nanoparticles(BSNs)were developed for syncretic bone repairing and anti-tumor treatment,which can enhance bone regeneration through the osteogenic effects of Cu^(2+) and Mn^(3+) ions and meanwhile induce tumor cells apoptosis through the hydroxyl radicals produced by the Fenton-like reactions of Cu^(2+) and Mn^(3+) ions.In vitro study showed that both osteogenic differentiation of BMSCs and angiogenesis of endothelial cells were promoted by BSNs,and consistently the critical bone defects of rats were efficiently repaired by BSNs through in vivo evaluation.Meanwhile,BSNs could generate hydroxyl radicals through Fenton-like reactions in the simulated tumor microenvironment,promote the generation of intracellular reactive oxygen species,and eventually induce tumor cell apoptosis.Besides,subcutaneous tumors of mice were effectively inhibited by BSNs without causing toxic side effects to normal tissues and organs.Altogether,Cu and Mn-doped BSNs developed in this work performed dual functions of enhancing osteogenesis and angiogenesis for bone regeneration,and inhibiting tumor growth for chemodynamic therapy,thus holding a great potential for syncretic bone repairing and anti-tumor therapy.
基金This project is financially supported by the Natural Science Foundation ofHeilongjiang Province (No. E0232)
文摘A new method of preparation of biomaterial composite coating by the techniqueof subsonic thermal spraying was discussed in this paper. Ti_6Al_4V and pure Ti were chosen assubstrate and sublayer material respectively and the working layer was sprayed with biomaterialhydroxyapatite (HAP), forming the composite coating. The experiments of heat shock and tensilestrength showed that the bonding strength between coating and substrate is almost as same as that ofspecimen in which Ni/Al powder was adopted as sublayer. The phases of TiN, TiO_2, and Ti_2O_3 wereformed in the sublayer, which are free of toxic and have no side effects. The powder of workinglayer HAP was decomposed partly during spraying, but it can be solved by later treatment.
文摘Hydroxyapatite (HA) bio-ceramics implant tooth was tested through animal experiment and clinical application. HA tooth was placed into the fresh extraction socket. At first, HA tooth was chosen and implanted as fixed maxillary front tooth in domestic rabbit immediately after extraction of tooth. Three to six months later satisfactory osseointegration between implanted HA teeth and periodontinum was observed. Clinically, 28 teeth in 25 patients were implanted with suitable HA teeth, chosen according to dental X-ray and models of different kinds of HA dental root. Crowning prosthesis with visible lightsolidfied material made the HA tooth wholesome and nice looking. Implantation and fixation of the HA teeth into the fresh extraction sockets of all the patients were successful.
