A new, convenient and excellent yield procedure for the preparation of bis（indolyl）methanes （BIMs） by Friedel-Crafts reaction of indoles with imines in the presence of dithiocarbohydrazone Schiff base/ Zn（CIO4）2...A new, convenient and excellent yield procedure for the preparation of bis（indolyl）methanes （BIMs） by Friedel-Crafts reaction of indoles with imines in the presence of dithiocarbohydrazone Schiff base/ Zn（CIO4）2.6H2O as a highly stable, effective and readily available catalyst is described. Moderate to good yields （up to 99% yield） were obtained with both substrates for imine derivatives. This procedure is simple, practical and economic.展开更多
Thin-walled lattice materials can be applied as energy absorbers in protective structures of civil defense. In this paper, quasi-static in-plane crushing tests were carried out to investigate the crushing behavior and...Thin-walled lattice materials can be applied as energy absorbers in protective structures of civil defense. In this paper, quasi-static in-plane crushing tests were carried out to investigate the crushing behavior and energy absorption of buckling induced meta-lattice structures (BIMSs) with different central angles made of plastic iron material DT3 and formed by wire cutting technique. Three crushing patterns were revealed and analyzed. The test results clearly show that the initial peak force (IPF), the crushing force efficiency (CFE), the specific energy absorption (SEA) and the mean crushing force (MCF) can be substantially improved by introducing buckling pattern into the straight-walled lattice structure. The MCF of the BIMS was consistently predicted based on the simplified super folding element (SSFE) and the flattening element.展开更多
Objective To transfer pro-apoptotic BIM directly into tumor cells bypass the complicated biologica processes of BIM activation so as to reverse the chemoresistance of cancer cells. Methods BIMS was specifically amplif...Objective To transfer pro-apoptotic BIM directly into tumor cells bypass the complicated biologica processes of BIM activation so as to reverse the chemoresistance of cancer cells. Methods BIMS was specifically amplified from HL-60 cells by RT-PCR, confirmed to be correct by sequencing and cloned into shuttle vector pAdTrack-CMV carrying a green fluorescence protein gene to generate a recombinant plasmid pAdTrack-CMV-BIMS. This plasmid and adenovirus backbone plasmid pAdEasy-1 were linearized and electroporated into E.coli BJ5183 host bacteria to mediate homologous recombination. The positive clone was identified by restrict endonuclease digestion. The recombinant pAdEasy-CMV-BIMS was transferred into HEK293 cells for packaging and amplification. The successful construction of recombinant human BIMS adenovirus （Ad-BIMS） was demonstrated by Western blot. To test whether Ad-BIMS has the capability of inducing apoptosis of tumor cells, Ad-BIMS was used to infect GC resistant Burkitt lymphoma Raji cells. Results After infected for 2-5 days, BIMS expression in Raji cells was detected by RT-PCR and Western blot. The significant growth retardation and apoptosis of Raji cells were also observed by MTI- and flow cytometry. Conclusion These results indicated that BIMS might be a potential candidate of gene therapy for chemoresistant tumor cells.展开更多
基金the Xinjiang Autonomous Region Youth Science Foundation(No.2011211B05)
文摘A new, convenient and excellent yield procedure for the preparation of bis（indolyl）methanes （BIMs） by Friedel-Crafts reaction of indoles with imines in the presence of dithiocarbohydrazone Schiff base/ Zn（CIO4）2.6H2O as a highly stable, effective and readily available catalyst is described. Moderate to good yields （up to 99% yield） were obtained with both substrates for imine derivatives. This procedure is simple, practical and economic.
基金Supports from National Natural Science Foundation of China(11972184 and U20A20286)China National Key Laboratory Foundation of Science and Technology on Materials under Shock and Impact(6142902200203)+1 种基金Natural Science Foundation of Jiangsu Province of China(BK20201286)Science and Technology Project of Jiangsu Province of China(BE2020716)are gratefully acknowledged.
文摘Thin-walled lattice materials can be applied as energy absorbers in protective structures of civil defense. In this paper, quasi-static in-plane crushing tests were carried out to investigate the crushing behavior and energy absorption of buckling induced meta-lattice structures (BIMSs) with different central angles made of plastic iron material DT3 and formed by wire cutting technique. Three crushing patterns were revealed and analyzed. The test results clearly show that the initial peak force (IPF), the crushing force efficiency (CFE), the specific energy absorption (SEA) and the mean crushing force (MCF) can be substantially improved by introducing buckling pattern into the straight-walled lattice structure. The MCF of the BIMS was consistently predicted based on the simplified super folding element (SSFE) and the flattening element.
文摘Objective To transfer pro-apoptotic BIM directly into tumor cells bypass the complicated biologica processes of BIM activation so as to reverse the chemoresistance of cancer cells. Methods BIMS was specifically amplified from HL-60 cells by RT-PCR, confirmed to be correct by sequencing and cloned into shuttle vector pAdTrack-CMV carrying a green fluorescence protein gene to generate a recombinant plasmid pAdTrack-CMV-BIMS. This plasmid and adenovirus backbone plasmid pAdEasy-1 were linearized and electroporated into E.coli BJ5183 host bacteria to mediate homologous recombination. The positive clone was identified by restrict endonuclease digestion. The recombinant pAdEasy-CMV-BIMS was transferred into HEK293 cells for packaging and amplification. The successful construction of recombinant human BIMS adenovirus （Ad-BIMS） was demonstrated by Western blot. To test whether Ad-BIMS has the capability of inducing apoptosis of tumor cells, Ad-BIMS was used to infect GC resistant Burkitt lymphoma Raji cells. Results After infected for 2-5 days, BIMS expression in Raji cells was detected by RT-PCR and Western blot. The significant growth retardation and apoptosis of Raji cells were also observed by MTI- and flow cytometry. Conclusion These results indicated that BIMS might be a potential candidate of gene therapy for chemoresistant tumor cells.
