Glycerol dialkyl glycerol tetraethers(GDGTs) in lake sediments are useful biomarkers for the continental paleoclimatic reconstruction. However, the environmental controls on the distribution of these compounds, in par...Glycerol dialkyl glycerol tetraethers(GDGTs) in lake sediments are useful biomarkers for the continental paleoclimatic reconstruction. However, the environmental controls on the distribution of these compounds, in particular the 6-methyl isomers of bacterial branched GDGTs(bGDGTs), in the lakes with contrasting pH, are still unknown, hindering their application for paleo-reconstructions. Here, we investigated the environmental impacts on the distribution of GDGTs in 17 alkaline lakes and 1 acid lake in China. It was found that the dissolved oxygen content in water column may have an impact on the distribution of archaeal isoprenoid GDGTs(iGDGTs) by causing the change in archaeal communities. The ratio of GDGT-0/crenarchaeol increases with decreasing oxygen content, indicating that the relative abundance of anaerobic methanogenic archaea or Miscellaneous Crenarchaeotic Group(MCG) vs. aerobic Thaumarchaeota is controlled by the oxygen content dissolved in water of these lakes. Thaumarchaeota are likely to contribute only a small proportion of iGDGTs in the relatively oxygen-depleted lakes, and thus TEX_(86) is not suitable for the reconstruction of the surface temperature of these lakes. The abundance ratio of iGDGTs to bGDGTs(R_(i/b)) appears to show no relationship with water pH in all the lakes, but exhibits a significant positive correlation with the water depth of the acid Lake Qinghai in Tengchong. As expected, the methylation degree of bGDGTs(MBT′) was found to correlate with both mean annual air temperature(MAT) and water pH, and the cyclization degree of bGDGTs(CBT) correlates only with water pH in these lakes. However, the MBT′_(5ME), an index to measure the methylation degree of 5-methyl bGDGTs, exhibits no relationship with MAT, whereas MBT′_(6ME), the methylation degree of 6-methyl bGDGTs, was found to correlate significantly with MAT. This is opposite to the situation observed in the global soils, pointing to a different adaptation of b GDGT-producing bacteria to environmental variables or diffe展开更多
为深入了解bGDGTs化合物对温度的响应是否受季节性变化和pH差异的影响,我们对中国多个偏碱性土壤bGDGTs化合物进行为期一年的观测.研究结果表明:在一年内,bGDGTs的组成对温度的响应是长期积累的结果,并不能反映短期(季节)的温度变化.寒...为深入了解bGDGTs化合物对温度的响应是否受季节性变化和pH差异的影响,我们对中国多个偏碱性土壤bGDGTs化合物进行为期一年的观测.研究结果表明:在一年内,bGDGTs的组成对温度的响应是长期积累的结果,并不能反映短期(季节)的温度变化.寒冷的青藏高原高海拔地区b GDGTs指标可能反映了春季/夏季过渡阶段的温度;而其他区域bGDGTs的分布和指标均未呈现明显的季节性特征.土壤pH是造成不同校正公式斜率差异的最主要原因,不同pH条件下,MBT指标对温度的响应存在显著差异.在中国中性-偏碱性土壤中,MBT指标与年均大气温度MAAT(5~25?C)的关系为:MAAT=20.78×MBT+5.92(R2=0.88, p <0.001).该校正公式在中性-偏碱性土壤环境中比之前的MBT-MAAT校正公式更精确,为反演中国区域古温度提供理论依据.展开更多
Branched glycerol dialkyl glycerol tetraethers （bGDGTs） are ubiquitous and abundant in soils, but their sources remain elusive. Recent studies demonstrate that the distributions of bGDGTs are sensitive to various en...Branched glycerol dialkyl glycerol tetraethers （bGDGTs） are ubiquitous and abundant in soils, but their sources remain elusive. Recent studies demonstrate that the distributions of bGDGTs are sensitive to various environmental factors. In an effort to understand how and to what extent soil moisture （expressed as soil water content （SWC） or mean annual precipita- tion （MAP）, pH and temperature may impact the distribution of bGDGTs, and to shed more light on the biological sources of bGDGTs in cold and arid regions, we investigated the distribution of bGDGTs as well as bacterial 16S rRNA gene and func- tional genes involved in the N cycle （including amoA, nirS and nirK） in 41 surface soil samples from around Lake Qinghai and east of Qaidam Basin on the Northeastern Qinghai-Tibetan Plateau. We found that lower soil moisture reduced the fractional concentrations of cyclic bGDGTs and thus the cyclisation ratio of branched tetraethers （CBT） index correlated negatively with SWC and MAP, suggesting that soil moisture is an important factor controlling bGDGT distributions in soils in this add and semi-add region. Two subgroups of bGDGTs were assigned on the basis of cluster analysis, and bGDGT indices behaved dif- ferently in the two groups, hinting at different biosynthetic mechanisms for bGDGTs under different environmental conditions. Real time PCR results showed that nirS and nirK genes correlated significantly with the concentration of bGDGTs, suggesting that the nirS- or nirK-encoding bacteria involved in denitrification might potentially be an additional biological source for soil bGDGTs （besides Acidobacteria）. Moreover, our results also support the application of new indices based on 5-methyl bGDGTs and 6-methyl bGDGTs in reconstructing past temperature and pH variations in this region.展开更多
基金supported by the Fundamental Research Funds for the Central Universities (Grant No. CUGL150812)the National Natural Science Foundation of China (Grant No. 41330103)the Programme of Introducing Talents of Discipline to Universities Project (Grant No. B08030)
文摘Glycerol dialkyl glycerol tetraethers(GDGTs) in lake sediments are useful biomarkers for the continental paleoclimatic reconstruction. However, the environmental controls on the distribution of these compounds, in particular the 6-methyl isomers of bacterial branched GDGTs(bGDGTs), in the lakes with contrasting pH, are still unknown, hindering their application for paleo-reconstructions. Here, we investigated the environmental impacts on the distribution of GDGTs in 17 alkaline lakes and 1 acid lake in China. It was found that the dissolved oxygen content in water column may have an impact on the distribution of archaeal isoprenoid GDGTs(iGDGTs) by causing the change in archaeal communities. The ratio of GDGT-0/crenarchaeol increases with decreasing oxygen content, indicating that the relative abundance of anaerobic methanogenic archaea or Miscellaneous Crenarchaeotic Group(MCG) vs. aerobic Thaumarchaeota is controlled by the oxygen content dissolved in water of these lakes. Thaumarchaeota are likely to contribute only a small proportion of iGDGTs in the relatively oxygen-depleted lakes, and thus TEX_(86) is not suitable for the reconstruction of the surface temperature of these lakes. The abundance ratio of iGDGTs to bGDGTs(R_(i/b)) appears to show no relationship with water pH in all the lakes, but exhibits a significant positive correlation with the water depth of the acid Lake Qinghai in Tengchong. As expected, the methylation degree of bGDGTs(MBT′) was found to correlate with both mean annual air temperature(MAT) and water pH, and the cyclization degree of bGDGTs(CBT) correlates only with water pH in these lakes. However, the MBT′_(5ME), an index to measure the methylation degree of 5-methyl bGDGTs, exhibits no relationship with MAT, whereas MBT′_(6ME), the methylation degree of 6-methyl bGDGTs, was found to correlate significantly with MAT. This is opposite to the situation observed in the global soils, pointing to a different adaptation of b GDGT-producing bacteria to environmental variables or diffe
文摘为深入了解bGDGTs化合物对温度的响应是否受季节性变化和pH差异的影响,我们对中国多个偏碱性土壤bGDGTs化合物进行为期一年的观测.研究结果表明:在一年内,bGDGTs的组成对温度的响应是长期积累的结果,并不能反映短期(季节)的温度变化.寒冷的青藏高原高海拔地区b GDGTs指标可能反映了春季/夏季过渡阶段的温度;而其他区域bGDGTs的分布和指标均未呈现明显的季节性特征.土壤pH是造成不同校正公式斜率差异的最主要原因,不同pH条件下,MBT指标对温度的响应存在显著差异.在中国中性-偏碱性土壤中,MBT指标与年均大气温度MAAT(5~25?C)的关系为:MAAT=20.78×MBT+5.92(R2=0.88, p <0.001).该校正公式在中性-偏碱性土壤环境中比之前的MBT-MAAT校正公式更精确,为反演中国区域古温度提供理论依据.
基金supported by the National Natural Science Foundation of China (Grant Nos. 41373072, 40873011 &41030211)the Shanghai Bureau of Science and Technology (Grant No. 13JC1405200)the Thousand Talents Program through the State Key Laboratory of Marine Geology at Tongji University (C. L. Zhang)
文摘Branched glycerol dialkyl glycerol tetraethers （bGDGTs） are ubiquitous and abundant in soils, but their sources remain elusive. Recent studies demonstrate that the distributions of bGDGTs are sensitive to various environmental factors. In an effort to understand how and to what extent soil moisture （expressed as soil water content （SWC） or mean annual precipita- tion （MAP）, pH and temperature may impact the distribution of bGDGTs, and to shed more light on the biological sources of bGDGTs in cold and arid regions, we investigated the distribution of bGDGTs as well as bacterial 16S rRNA gene and func- tional genes involved in the N cycle （including amoA, nirS and nirK） in 41 surface soil samples from around Lake Qinghai and east of Qaidam Basin on the Northeastern Qinghai-Tibetan Plateau. We found that lower soil moisture reduced the fractional concentrations of cyclic bGDGTs and thus the cyclisation ratio of branched tetraethers （CBT） index correlated negatively with SWC and MAP, suggesting that soil moisture is an important factor controlling bGDGT distributions in soils in this add and semi-add region. Two subgroups of bGDGTs were assigned on the basis of cluster analysis, and bGDGT indices behaved dif- ferently in the two groups, hinting at different biosynthetic mechanisms for bGDGTs under different environmental conditions. Real time PCR results showed that nirS and nirK genes correlated significantly with the concentration of bGDGTs, suggesting that the nirS- or nirK-encoding bacteria involved in denitrification might potentially be an additional biological source for soil bGDGTs （besides Acidobacteria）. Moreover, our results also support the application of new indices based on 5-methyl bGDGTs and 6-methyl bGDGTs in reconstructing past temperature and pH variations in this region.
