Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds a...Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds and used for human nutrition. For the first time, we have systematically investigated the effects of culture conditions in cylindrical glass columns and fiat-plate photobioreactors, including nutrients （nitrogen, phosphorus, silicon, and sulfur）, light intensity and light path, on O. aurita cell growth and biochemical composition （protein, carbohydrate, β-1,3-glucan, lipids, and ash）. The optimal medium for photoautotrophic cultivation of O. aurita contained 17.65 mmol/L nitrogen, 1.09 mmol/L phosphorus, 0.42 mmol/L silicon, and 24.51 mmol/L sulfur, yielding a maximum biomass production of 6.1-6.8 g/L and 6.7-7.8 g/L under low and high light, respectively. Scale-up experiments were conducted with fiat-plate photobioreactors using different light-paths, indicating that a short light path was more suitable for biomass production of O. aurita. Analyses of biochemical composition showed that protein content decreased while carbohydrate （mainly composed of 15-1,3-glucan） increased remarkably to about 50% of dry weight during the entire culture period. The highest lipid content （19.7% of dry weight） was obtained under 0.11 mmol/L silicon and high light conditions at harvest time. Fatty acid profiles revealed that 80% were Cx4, C^6, and C20, while arachidonic acid and eicosapentaenoic acid （EPA） accounted for 1.6%-5.6% and 9%-20% of total fatty acids, respectively. High biomass production and characteristic biochemical composition profiles make O. aurita a promising microalga for the production ofbioactive components, such as EPA and D-1,3-glucan.展开更多
Blooms of the scyphozoan jellyfish <i><span style="font-family:Verdana;">Aurelia aurita</span></i><span style="font-family:Verdana;"> are greatly regulated by the surv...Blooms of the scyphozoan jellyfish <i><span style="font-family:Verdana;">Aurelia aurita</span></i><span style="font-family:Verdana;"> are greatly regulated by the survival rate of planktonic ephyrae. The ecophysiology of ephyrae is poorly studied compared with polyps and medusae. As extremely strong starvation resistance and recovery capability of </span><i><span style="font-family:Verdana;">A. aurita</span></i><span style="font-family:Verdana;"> ephyrae may due to its low metabolic rate as well as starvation may reduce the swimming ability of ephyrae which may lead to the higher predation loss, the effects of temperature and starvation on their respiration and pulsation rates were examined. In this study, ephyrae under different starvation conditions were measured by a fluorescence-based DO meter after 72 h incubation. And the pulsation rates were measured at every 10-d interval in 1-liter plastic bottle by a hand-held counter. The results showed that the mean respiration rates of newly released ephyrae were 0.24, 0.24 and 0.19 μl O</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> ephyra</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;"> d</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;"> at 15°C, 12°C and 9°C, respectively, and the rates tended to decrease with increasing starvation duration. Carbon weight-specific respiration rates did not change significantly with starvation duration. The dry weight-specific respiration rates of newly released </span><i><span style="font-family:Verdana;">A. aurita</span></i><span style="font-family:Verdana;"> ephyrae (</span><i><span style="font-family:Verdana;">i.e.</span></i><span style="font-family:Verdana;">, 11.7</span><span style="font-size:10pt;font-family:Verdana;">-</span><span style="font-size:10pt;font-family:;" "=""><span style="font-family:Verdana;">14.6 μl O</span><sub><span style="font-family:Verdana;">2</span></sub><s展开更多
The current study explored Aurelia aurita and Rhopilema esculent um consumption by silver pomfret juveniles, as well as their prey selection between the two jellyfi sh species. Silver pomfret juveniles weighing 1±...The current study explored Aurelia aurita and Rhopilema esculent um consumption by silver pomfret juveniles, as well as their prey selection between the two jellyfi sh species. Silver pomfret juveniles weighing 1±0.1 g actively preyed on both the species. Their daily A. aurita consumption was 11.6 times their own body weights regardless of the size of A. aurita medusae. Their daily R. esculent um consumption was 13, 9.1, 5, and 4.1 times their own body weights when the R. esculentum medusae were 10, 20, 30, and 40 mm in bell diameter, respectively. The survival rates of the R. esculent um were higher than those of the A. aurita. When the R. esculent um medusae were more than 30 mm in bell diameter, their survival rate exceeded 92%. Silver pomfrets serve as a type of potential predators on A. aurita in coastal waters, and they have little infl uence on R. esculent um with a size exceeding 30 mm. Besides, A. aurita may be able to be used as fi sh prey in silver pomfret artifi cial breeding.展开更多
Scyphozoan jellyfish outbreak events are drawing increasing attentions during the past decade.Elemental compositions of statoliths are helpful to understand jellyfish life history and blooming mechanisms,but very rare...Scyphozoan jellyfish outbreak events are drawing increasing attentions during the past decade.Elemental compositions of statoliths are helpful to understand jellyfish life history and blooming mechanisms,but very rare endeavor has been focused on the Scyphozoan class.In this work,we explored the feasibility of element analysis of Aurelia aurita(a representative Scyphozoan jellyfish outbreak species in China)which may be used as proxies of environment parameters during jellyfish living and moving.Statolith crystals of Aurelia aurita were found to be a gathering of hexahedron type trigonal needle with size of 10-50μm long,and 5-10μm in diameter.By using laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS)downhole profiling method,elements such as Ca,Sr,Mg,Na and P were found to be above the limit of detection and limit of quantification.The epidermis of statocyst could significantly impact the element analyses,so the real statolith element signal section needs to be selected based on elements and Ca profiles together with care.By laser ablated a signal spot repeatedly,the analytical uncertainty was about 3%-4%for Sr/Ca content ratio and Mg/Ca content ratio,but above 10%for other element/Ca content ratios(n=3).Based on the analysis of statolith from temperature-control cultured jellyfish,Sr/Ca content ratios among different statoliths of the same jellyfish were about 6%(n=14),demonstrating biological processes/vital effects causing small variations compared with analytical uncertainties.Therefore,Sr/Ca content ratios may be used as a potential proxy to reveal the living environment variations the Scyphozoan jellyfish has experienced,such as temperature history,which is helpful to understand jellyfish bloom mechanisms.展开更多
Objective:To estimate the biological and population parameters required for proposing a preparation to sustain and manage this valuable fish resource.Methods:Aging was done by scales reading,and growth was estimated b...Objective:To estimate the biological and population parameters required for proposing a preparation to sustain and manage this valuable fish resource.Methods:Aging was done by scales reading,and growth was estimated by using the back-calculation method.The values of growth parameters L_(∞),k and t_(o)were calculated by von Bertalanffy model.Results:The results of growth parameters L_(∞),k and t_(o)were 28.36 cm,0.184 per year and-0.8437 per year,respectively.Mortality coefficient,survival and exploitation rates estimated to perceive yield per recruit and biomass per recruit.Conclusions:Biological reference points and virtual population analysis were prepared to plan appropriate managements forSardinella aurita fisheries.展开更多
Jellyfish outbreaks have become a new type of marine ecological disasters,and have great influence on the structure and function balance of marine ecosystems,fishery resources,production safety of coastal power plants...Jellyfish outbreaks have become a new type of marine ecological disasters,and have great influence on the structure and function balance of marine ecosystems,fishery resources,production safety of coastal power plants,marine landscape and tourism.In recent years,the Yellow Sea and the northern East China Sea are the high risk areas of jellyfish outbreaks.In this paper,the research results of jellyfish outbreaks in the Yellow Sea and the northern East China Sea are summarized,and the species composition,distribution,life-history characteristics and hazards of jellyfish outbreaks are described.Meanwhile,the possible causes of jellyfish outbreaks are analyzed,and the monitoring and control technology of jellyfish disasters are discussed.It will provide a scientific basis for the monitoring,management,and emergency disposal of jellyfish disasters.展开更多
基金Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2009AA06440,2013AA065805)the National Basic Research Program of China(973 Program)(No.2011CB2009001)the National Natural Science Foundation of China(No.31170337)
文摘Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds and used for human nutrition. For the first time, we have systematically investigated the effects of culture conditions in cylindrical glass columns and fiat-plate photobioreactors, including nutrients （nitrogen, phosphorus, silicon, and sulfur）, light intensity and light path, on O. aurita cell growth and biochemical composition （protein, carbohydrate, β-1,3-glucan, lipids, and ash）. The optimal medium for photoautotrophic cultivation of O. aurita contained 17.65 mmol/L nitrogen, 1.09 mmol/L phosphorus, 0.42 mmol/L silicon, and 24.51 mmol/L sulfur, yielding a maximum biomass production of 6.1-6.8 g/L and 6.7-7.8 g/L under low and high light, respectively. Scale-up experiments were conducted with fiat-plate photobioreactors using different light-paths, indicating that a short light path was more suitable for biomass production of O. aurita. Analyses of biochemical composition showed that protein content decreased while carbohydrate （mainly composed of 15-1,3-glucan） increased remarkably to about 50% of dry weight during the entire culture period. The highest lipid content （19.7% of dry weight） was obtained under 0.11 mmol/L silicon and high light conditions at harvest time. Fatty acid profiles revealed that 80% were Cx4, C^6, and C20, while arachidonic acid and eicosapentaenoic acid （EPA） accounted for 1.6%-5.6% and 9%-20% of total fatty acids, respectively. High biomass production and characteristic biochemical composition profiles make O. aurita a promising microalga for the production ofbioactive components, such as EPA and D-1,3-glucan.
文摘Blooms of the scyphozoan jellyfish <i><span style="font-family:Verdana;">Aurelia aurita</span></i><span style="font-family:Verdana;"> are greatly regulated by the survival rate of planktonic ephyrae. The ecophysiology of ephyrae is poorly studied compared with polyps and medusae. As extremely strong starvation resistance and recovery capability of </span><i><span style="font-family:Verdana;">A. aurita</span></i><span style="font-family:Verdana;"> ephyrae may due to its low metabolic rate as well as starvation may reduce the swimming ability of ephyrae which may lead to the higher predation loss, the effects of temperature and starvation on their respiration and pulsation rates were examined. In this study, ephyrae under different starvation conditions were measured by a fluorescence-based DO meter after 72 h incubation. And the pulsation rates were measured at every 10-d interval in 1-liter plastic bottle by a hand-held counter. The results showed that the mean respiration rates of newly released ephyrae were 0.24, 0.24 and 0.19 μl O</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> ephyra</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;"> d</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;"> at 15°C, 12°C and 9°C, respectively, and the rates tended to decrease with increasing starvation duration. Carbon weight-specific respiration rates did not change significantly with starvation duration. The dry weight-specific respiration rates of newly released </span><i><span style="font-family:Verdana;">A. aurita</span></i><span style="font-family:Verdana;"> ephyrae (</span><i><span style="font-family:Verdana;">i.e.</span></i><span style="font-family:Verdana;">, 11.7</span><span style="font-size:10pt;font-family:Verdana;">-</span><span style="font-size:10pt;font-family:;" "=""><span style="font-family:Verdana;">14.6 μl O</span><sub><span style="font-family:Verdana;">2</span></sub><s
基金Supported by National Basic Research Program of China(973 Program)(No.2011CB403605)the Key Science and Technology Program of Qingdao(No.11-3-1-6-hy)+1 种基金the Special Funds for the Basic R&D Programin the Central Non-Profit Research Institutes(No.20603022011008)the National Key Technology R&D Program during the Twelfth Five-Year Plan Period(No.2011BAD13B01)
文摘The current study explored Aurelia aurita and Rhopilema esculent um consumption by silver pomfret juveniles, as well as their prey selection between the two jellyfi sh species. Silver pomfret juveniles weighing 1±0.1 g actively preyed on both the species. Their daily A. aurita consumption was 11.6 times their own body weights regardless of the size of A. aurita medusae. Their daily R. esculent um consumption was 13, 9.1, 5, and 4.1 times their own body weights when the R. esculentum medusae were 10, 20, 30, and 40 mm in bell diameter, respectively. The survival rates of the R. esculent um were higher than those of the A. aurita. When the R. esculent um medusae were more than 30 mm in bell diameter, their survival rate exceeded 92%. Silver pomfrets serve as a type of potential predators on A. aurita in coastal waters, and they have little infl uence on R. esculent um with a size exceeding 30 mm. Besides, A. aurita may be able to be used as fi sh prey in silver pomfret artifi cial breeding.
基金The National Key Research and Development Program of China under contract No.2017YFC1404402the National Natural Science Foundation of China under contract Nos U1906210 and 41876075。
文摘Scyphozoan jellyfish outbreak events are drawing increasing attentions during the past decade.Elemental compositions of statoliths are helpful to understand jellyfish life history and blooming mechanisms,but very rare endeavor has been focused on the Scyphozoan class.In this work,we explored the feasibility of element analysis of Aurelia aurita(a representative Scyphozoan jellyfish outbreak species in China)which may be used as proxies of environment parameters during jellyfish living and moving.Statolith crystals of Aurelia aurita were found to be a gathering of hexahedron type trigonal needle with size of 10-50μm long,and 5-10μm in diameter.By using laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS)downhole profiling method,elements such as Ca,Sr,Mg,Na and P were found to be above the limit of detection and limit of quantification.The epidermis of statocyst could significantly impact the element analyses,so the real statolith element signal section needs to be selected based on elements and Ca profiles together with care.By laser ablated a signal spot repeatedly,the analytical uncertainty was about 3%-4%for Sr/Ca content ratio and Mg/Ca content ratio,but above 10%for other element/Ca content ratios(n=3).Based on the analysis of statolith from temperature-control cultured jellyfish,Sr/Ca content ratios among different statoliths of the same jellyfish were about 6%(n=14),demonstrating biological processes/vital effects causing small variations compared with analytical uncertainties.Therefore,Sr/Ca content ratios may be used as a potential proxy to reveal the living environment variations the Scyphozoan jellyfish has experienced,such as temperature history,which is helpful to understand jellyfish bloom mechanisms.
基金supported by General Authority for Fish Resources Development(GAFRD),Egypt.
文摘Objective:To estimate the biological and population parameters required for proposing a preparation to sustain and manage this valuable fish resource.Methods:Aging was done by scales reading,and growth was estimated by using the back-calculation method.The values of growth parameters L_(∞),k and t_(o)were calculated by von Bertalanffy model.Results:The results of growth parameters L_(∞),k and t_(o)were 28.36 cm,0.184 per year and-0.8437 per year,respectively.Mortality coefficient,survival and exploitation rates estimated to perceive yield per recruit and biomass per recruit.Conclusions:Biological reference points and virtual population analysis were prepared to plan appropriate managements forSardinella aurita fisheries.
基金Supported by Special Project of National Key Research and Development Program of China(2016YFC1402201).
文摘Jellyfish outbreaks have become a new type of marine ecological disasters,and have great influence on the structure and function balance of marine ecosystems,fishery resources,production safety of coastal power plants,marine landscape and tourism.In recent years,the Yellow Sea and the northern East China Sea are the high risk areas of jellyfish outbreaks.In this paper,the research results of jellyfish outbreaks in the Yellow Sea and the northern East China Sea are summarized,and the species composition,distribution,life-history characteristics and hazards of jellyfish outbreaks are described.Meanwhile,the possible causes of jellyfish outbreaks are analyzed,and the monitoring and control technology of jellyfish disasters are discussed.It will provide a scientific basis for the monitoring,management,and emergency disposal of jellyfish disasters.
