A new type of photoelectrochemical cathodic protection technology(a combination of seawater corrosion and biological fouling resistance)is being actively researched to alleviate the serious corrosion of marine metal m...A new type of photoelectrochemical cathodic protection technology(a combination of seawater corrosion and biological fouling resistance)is being actively researched to alleviate the serious corrosion of marine metal materials.At present,there is almost no research on anti-corrosion and anti-fouling dual functional materials.In this paper,Cu_(2)ZnSnS_(4)is attached to the surface of TiO,nanotubes through a one-step hydrothermal method for modification.The results indicate that when the hydrothermal reaction time is 24 h,Cu_(2)ZnSnS_(4)/TiO_(2)nanocomposite material exhibits excellent performance in coupling with the protected 304 SS,with its open circuit potential shifts negatively to-1.04 V.This material improves the separation efficiency of photogenerated electrons and effectively improves the photochemical cathodic protection of 304 stainless steel.The high removal rate of Staphylococcus aureus(up to 93%)of the as-prepared samples also proved that it has the effect of the anti-biological fouling.展开更多
Background: Staphylococcus aureus is one of the most virulent gram positive bacteria. It produces a lot of toxins and enzymes, most of which are virulent factors. Among the enzyme that produces is the catalase which i...Background: Staphylococcus aureus is one of the most virulent gram positive bacteria. It produces a lot of toxins and enzymes, most of which are virulent factors. Among the enzyme that produces is the catalase which is very useful in differentiating staphylococci from streptococci [1]. Catalase is nearly ubiquitous among some of organisms that can grow in the presence of oxygen (air). It promotes the conversion of hydrogen peroxide, a powerful and potentially harmful oxidizing agent, to water and molecular oxygen;so the major function of catalase within cells is to prevent the accumulation of toxic levels of hydrogen peroxide formed as a by-product of metabolic processes—primarily that of the electron transport pathway. Objectives: The main aim of this study is to prove that human WBCs can produce H2O2. This H2O2 when reacting with catalase producing S. aureus can easily be degraded to H2O + O2. Methodology: In this study a total of 40 subjects were included. Aliquots of 2.5 ml of venous blood were collected by venous puncture after disinfecting the site of collection with 70% alcohol and the collected blood was drawn into EDITA containers (20 subject) and anticoagulant free containers (other 20 subject), centrifugation for 5 minute at 1500 RPM. The separated sera and plasma were converted to new sterile eppendrof tubes and freezing until used (we leaved the eppendrof tubes that contained sera and plasma at room temperature before using it for DE freezing). Standard catalase producing S. aureus were used by taking 1 colony from Macconkey media by using applicator wooden stick, and inserted in eppendrof tube, then air bubbles would appear to indicate occurrence of the reactions. Results: According to this study, it was proved that WBCs in human plasma or serum can produce H2O2;this H2O2 was reacted with catalase enzyme produce from colony of S. aureus to produce air bubbles and water. There were no differences between using H2O2 or human plasma/serum that contains WBCs to detect and identify S. aureus by both te展开更多
The continuous search for an antimicrobial agent led to the identification of potential antimicrobial biomaterials based on polymers naturals, such as chitosan (CS). However, the mechanism of action of antibacterial a...The continuous search for an antimicrobial agent led to the identification of potential antimicrobial biomaterials based on polymers naturals, such as chitosan (CS). However, the mechanism of action of antibacterial activity of CS for gram-positive and gram-negative bacteria was not completely elucidated. The aim of this work is to report the antibacterial activity of CS through ultrastructural analyses of the clinical isolates Staphylococcus aureus and Escherichia coli by Transmission Electron Microscopy. The CS has a bactericidal action against S. aureus and E. coli which alters its cellular ultrastructure, such as with collapsed cell walls, condensed chromatin and the increase of intracellulares structures like vacuoles and cell debris. In this way, the CS represents a potential model for the future design of antibacterial in order to control bacterial resistance of patients in hospital settings.展开更多
Aim: To evaluate morbidity and mortality rate, clinical cure rate and cost of linezolid versus vancomycin in patients who have hospital-acquired pneumonia (HAP), ventilator-associated pneumonia (VAP) or Healthcare-ass...Aim: To evaluate morbidity and mortality rate, clinical cure rate and cost of linezolid versus vancomycin in patients who have hospital-acquired pneumonia (HAP), ventilator-associated pneumonia (VAP) or Healthcare-associated pneumonia (HCAP) caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Retrospective analysis data. Data were collected for adult patients admitted to King Faisal Specialist Hospital and Research Centre-Jeddah (KFSH & RC-J) from January 2010 to May 2015. Method: A total of 88 patients with HAP, VAP and HCAP caused by MRSA treated with vancomycin (IV) or linezolid (IV or PO) either as empirically or directed therapy ≥ 7 days. They are retrospectively evaluated and analyzed. The primary end points are morbidity and mortality rate as well as clinical cure rate. The secondary end point is the cost analysis for each medication. Results: A total of 40 patients (ICU, n = 13 (32.5% and non ICU, n = 27 (67.5%)) were included in the study. Among vancomycin, n = 21 (52.5%);age (54.95 ± 18.255) and linezolid, n = 19 (74.5%);age (48.684 ± 25.593), there was no statistical differences in mortality and morbidity rate (P = 0.375). Clinical cure rate (fever improvement, 12 (57.1%) vs 12 (63.2%);P = 0.698, leukocytosis improvement, 15 (71.4%) vs 14 (73.7%);P = 0.873, purulent sputum improvement, 6 (28.6%) vs 4 (21.1%);P = 0.429, dyspnea improvement, 8 (38.1%) vs 3 (15.8%);P = 0.115,cough improvement 4 (19.0%) vs 4 (21.1%);P = 0.592, microbiological eradication of MRSA from sputum culture, 2 (9.5%) vs 6 (31.6%);P = 0.089 and improvement of radiographic finding (pulmonary infiltration), 17 (81.0%) vs 16 (84.2%);P = 0.559) of vancomycin vs linezolid, respectively. The cost analysis in the treatment of MRSA pneumonia with linezolid is statistical significantly higher than vancomycin. The mean cost of vancomycin = 185.9143 SR and of linezolid = 4547.3684 SR (P Conclusion: There are no statistical differences in mortality and morbidity rate and clinical cure rate between linezolid and vancomycin展开更多
基金Projects(42106051,U2106206)supported by the National Natural Science Foundation of China。
文摘A new type of photoelectrochemical cathodic protection technology(a combination of seawater corrosion and biological fouling resistance)is being actively researched to alleviate the serious corrosion of marine metal materials.At present,there is almost no research on anti-corrosion and anti-fouling dual functional materials.In this paper,Cu_(2)ZnSnS_(4)is attached to the surface of TiO,nanotubes through a one-step hydrothermal method for modification.The results indicate that when the hydrothermal reaction time is 24 h,Cu_(2)ZnSnS_(4)/TiO_(2)nanocomposite material exhibits excellent performance in coupling with the protected 304 SS,with its open circuit potential shifts negatively to-1.04 V.This material improves the separation efficiency of photogenerated electrons and effectively improves the photochemical cathodic protection of 304 stainless steel.The high removal rate of Staphylococcus aureus(up to 93%)of the as-prepared samples also proved that it has the effect of the anti-biological fouling.
文摘Background: Staphylococcus aureus is one of the most virulent gram positive bacteria. It produces a lot of toxins and enzymes, most of which are virulent factors. Among the enzyme that produces is the catalase which is very useful in differentiating staphylococci from streptococci [1]. Catalase is nearly ubiquitous among some of organisms that can grow in the presence of oxygen (air). It promotes the conversion of hydrogen peroxide, a powerful and potentially harmful oxidizing agent, to water and molecular oxygen;so the major function of catalase within cells is to prevent the accumulation of toxic levels of hydrogen peroxide formed as a by-product of metabolic processes—primarily that of the electron transport pathway. Objectives: The main aim of this study is to prove that human WBCs can produce H2O2. This H2O2 when reacting with catalase producing S. aureus can easily be degraded to H2O + O2. Methodology: In this study a total of 40 subjects were included. Aliquots of 2.5 ml of venous blood were collected by venous puncture after disinfecting the site of collection with 70% alcohol and the collected blood was drawn into EDITA containers (20 subject) and anticoagulant free containers (other 20 subject), centrifugation for 5 minute at 1500 RPM. The separated sera and plasma were converted to new sterile eppendrof tubes and freezing until used (we leaved the eppendrof tubes that contained sera and plasma at room temperature before using it for DE freezing). Standard catalase producing S. aureus were used by taking 1 colony from Macconkey media by using applicator wooden stick, and inserted in eppendrof tube, then air bubbles would appear to indicate occurrence of the reactions. Results: According to this study, it was proved that WBCs in human plasma or serum can produce H2O2;this H2O2 was reacted with catalase enzyme produce from colony of S. aureus to produce air bubbles and water. There were no differences between using H2O2 or human plasma/serum that contains WBCs to detect and identify S. aureus by both te
文摘The continuous search for an antimicrobial agent led to the identification of potential antimicrobial biomaterials based on polymers naturals, such as chitosan (CS). However, the mechanism of action of antibacterial activity of CS for gram-positive and gram-negative bacteria was not completely elucidated. The aim of this work is to report the antibacterial activity of CS through ultrastructural analyses of the clinical isolates Staphylococcus aureus and Escherichia coli by Transmission Electron Microscopy. The CS has a bactericidal action against S. aureus and E. coli which alters its cellular ultrastructure, such as with collapsed cell walls, condensed chromatin and the increase of intracellulares structures like vacuoles and cell debris. In this way, the CS represents a potential model for the future design of antibacterial in order to control bacterial resistance of patients in hospital settings.
文摘Aim: To evaluate morbidity and mortality rate, clinical cure rate and cost of linezolid versus vancomycin in patients who have hospital-acquired pneumonia (HAP), ventilator-associated pneumonia (VAP) or Healthcare-associated pneumonia (HCAP) caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Retrospective analysis data. Data were collected for adult patients admitted to King Faisal Specialist Hospital and Research Centre-Jeddah (KFSH & RC-J) from January 2010 to May 2015. Method: A total of 88 patients with HAP, VAP and HCAP caused by MRSA treated with vancomycin (IV) or linezolid (IV or PO) either as empirically or directed therapy ≥ 7 days. They are retrospectively evaluated and analyzed. The primary end points are morbidity and mortality rate as well as clinical cure rate. The secondary end point is the cost analysis for each medication. Results: A total of 40 patients (ICU, n = 13 (32.5% and non ICU, n = 27 (67.5%)) were included in the study. Among vancomycin, n = 21 (52.5%);age (54.95 ± 18.255) and linezolid, n = 19 (74.5%);age (48.684 ± 25.593), there was no statistical differences in mortality and morbidity rate (P = 0.375). Clinical cure rate (fever improvement, 12 (57.1%) vs 12 (63.2%);P = 0.698, leukocytosis improvement, 15 (71.4%) vs 14 (73.7%);P = 0.873, purulent sputum improvement, 6 (28.6%) vs 4 (21.1%);P = 0.429, dyspnea improvement, 8 (38.1%) vs 3 (15.8%);P = 0.115,cough improvement 4 (19.0%) vs 4 (21.1%);P = 0.592, microbiological eradication of MRSA from sputum culture, 2 (9.5%) vs 6 (31.6%);P = 0.089 and improvement of radiographic finding (pulmonary infiltration), 17 (81.0%) vs 16 (84.2%);P = 0.559) of vancomycin vs linezolid, respectively. The cost analysis in the treatment of MRSA pneumonia with linezolid is statistical significantly higher than vancomycin. The mean cost of vancomycin = 185.9143 SR and of linezolid = 4547.3684 SR (P Conclusion: There are no statistical differences in mortality and morbidity rate and clinical cure rate between linezolid and vancomycin
