Background Our previous study confirmed that oligodendrogliomas had higher frequency of chromosome 1p/19q deletion. In order to improve the diagnostic criteria and to predict the prognosis of oligodendroglioma patient...Background Our previous study confirmed that oligodendrogliomas had higher frequency of chromosome 1p/19q deletion. In order to improve the diagnostic criteria and to predict the prognosis of oligodendroglioma patients, the status of chromosome 1 p/19q deletion, the methylation of O6-methylguanine-DNA methyltransferase （MGMT）, and the expression of p53 protein were evaluated and investigated in relation to patients＇ outcomes.Methods Methylation of MGMT in 73 cases was analyzed by nested methylation-specific PCR （MSP）. The levels of MGMT and p53 protein were tested with immunohistochemistry. Pearson＇s chi-square test and Fisher＇s exact test were used. Multivariate and Kaplan-Meier analysis were performed to determine patients＇ outcomes.Results Both oligodendrogliomas and astrocytic gliomas exhibited frequent methylation of MGMT. However, the results of MSP did not completely correspond to that of the immunohistochemical staining for MGMT. The expression of p53 protein was more frequently observed in patients without a 1 p or 19q deletion in anaplastic oligodendrogliomas （=0.032,0.025）. In low-grade oligodendrogliomas, methylation of MGMT was more frequent in patients with 1 p/19q deletion than in patients with 1p/19q intact （P=0.038）. Patients with oligodendrogliomas with 1p/19q loss of heterozygosity and p53-negative showed a longer progression-free survival.Conclusion Detection of chromosome 1p/19q status combined with p53 protein immunohistochemistry might be beneficial to improve the pathological diagnosis and to determine the prognosis of patients with oligodendrogliomas.展开更多
目的研究肿瘤微环境中肿瘤相关巨噬细胞(TAM)对星形胶质瘤增殖的影响。方法用免疫组化方法检测不同病理级别的星形胶质瘤组织中CD68阳性TAM的浸润情况,用Imaga-Pro Plus 6.0软件分析每个视野阳性染色的光密度积分(IOD),每个组织切片在40...目的研究肿瘤微环境中肿瘤相关巨噬细胞(TAM)对星形胶质瘤增殖的影响。方法用免疫组化方法检测不同病理级别的星形胶质瘤组织中CD68阳性TAM的浸润情况,用Imaga-Pro Plus 6.0软件分析每个视野阳性染色的光密度积分(IOD),每个组织切片在400倍下分析20个视野。结果高病理级别的星形胶质瘤组织比低病理级别的星形胶质瘤组织CD68阳性的TAM数量明显增多,随着代表星形胶质瘤增殖的指标Ki67表达的增高,TAM在星形胶质瘤组织中的IOD明显升高。结论 TAM在肿瘤微环境中浸润的数量与星形胶质瘤增殖密切相关。展开更多
文摘Background Our previous study confirmed that oligodendrogliomas had higher frequency of chromosome 1p/19q deletion. In order to improve the diagnostic criteria and to predict the prognosis of oligodendroglioma patients, the status of chromosome 1 p/19q deletion, the methylation of O6-methylguanine-DNA methyltransferase （MGMT）, and the expression of p53 protein were evaluated and investigated in relation to patients＇ outcomes.Methods Methylation of MGMT in 73 cases was analyzed by nested methylation-specific PCR （MSP）. The levels of MGMT and p53 protein were tested with immunohistochemistry. Pearson＇s chi-square test and Fisher＇s exact test were used. Multivariate and Kaplan-Meier analysis were performed to determine patients＇ outcomes.Results Both oligodendrogliomas and astrocytic gliomas exhibited frequent methylation of MGMT. However, the results of MSP did not completely correspond to that of the immunohistochemical staining for MGMT. The expression of p53 protein was more frequently observed in patients without a 1 p or 19q deletion in anaplastic oligodendrogliomas （=0.032,0.025）. In low-grade oligodendrogliomas, methylation of MGMT was more frequent in patients with 1 p/19q deletion than in patients with 1p/19q intact （P=0.038）. Patients with oligodendrogliomas with 1p/19q loss of heterozygosity and p53-negative showed a longer progression-free survival.Conclusion Detection of chromosome 1p/19q status combined with p53 protein immunohistochemistry might be beneficial to improve the pathological diagnosis and to determine the prognosis of patients with oligodendrogliomas.
文摘目的研究肿瘤微环境中肿瘤相关巨噬细胞(TAM)对星形胶质瘤增殖的影响。方法用免疫组化方法检测不同病理级别的星形胶质瘤组织中CD68阳性TAM的浸润情况,用Imaga-Pro Plus 6.0软件分析每个视野阳性染色的光密度积分(IOD),每个组织切片在400倍下分析20个视野。结果高病理级别的星形胶质瘤组织比低病理级别的星形胶质瘤组织CD68阳性的TAM数量明显增多,随着代表星形胶质瘤增殖的指标Ki67表达的增高,TAM在星形胶质瘤组织中的IOD明显升高。结论 TAM在肿瘤微环境中浸润的数量与星形胶质瘤增殖密切相关。
