Objective To determine the role of the circulating antisperm antibody （ASA） in the pathogenesis of missed abortion at the first-trimester pregnancy. Methods Sixty-two patients with a history of missed abortion at th...Objective To determine the role of the circulating antisperm antibody （ASA） in the pathogenesis of missed abortion at the first-trimester pregnancy. Methods Sixty-two patients with a history of missed abortion at the first-trimester pregnancy were enrolled into this study. Indirect immunobead test （IBT） was used to measure the circulating ASA levels. Fifty healthy women with the first-trimester pregnancy set as the control. Results No case had the positive level of ASA according to the World Health Organization criteria （50% or more of the motile sperm with immunobead binding）. Only 1 case in patient group and 1 case in the control had 10%-20% of the motile spermatozoa with ASA-IgG bead binding. In both patient and control groups, ASA-IgA was found to be completely negative binding. Conclusion The circulating ASA is not associated with the pathogenesis of missed abortions at the first-trimester pregnancy.展开更多
Objective To evaluate levels of the circulating antisperm antibody （ASA） in infertile women with polycystic ovary syndrome （PCOS）.Methods Forty-six infertile women with PCOS were enrolled into this study. The seru...Objective To evaluate levels of the circulating antisperm antibody （ASA） in infertile women with polycystic ovary syndrome （PCOS）.Methods Forty-six infertile women with PCOS were enrolled into this study. The serum was screened by the indirect immunobead test for ASA type IgG and IgA according to the WHO laboratory manual.Results Of the 46 patients assessed for ASA-IgG, 3 cases had immunobead binding which were 20%, 27% and 35%, respectively, and the sub-positive rate was 6.5%. No case had the clinical positive level according to the WHO criteria （50% or more of the motile sperm with immunobead binding）. ASA-IgA was not detected in all cases. Conclusion The circulating ASA is not associated with the pathogenesis of infertile women with PCOS.展开更多
Objective To investigate the role of circulating antisperm antibody （ASA） in the pathogenesis of endometriosis-associated infertility. Methods Serum samples were collected from 75 infertile women with minimal or mil...Objective To investigate the role of circulating antisperm antibody （ASA） in the pathogenesis of endometriosis-associated infertility. Methods Serum samples were collected from 75 infertile women with minimal or mild endometriosis. Indirect immunobead test reporting on the percentage of motile sperm with adherent immunobeads was used to detect the circulating ASA levels. Results No infertile cases enrolled in the present study developed significant ASA level in the serum samples. That is to say, no one could achieve the diagnosis of ASAmediated infertility according to the World Health Organization criteria （50% or more of the motile sperm with immunobead binding）. There were only 5 cases （6.7%） who had 20%-40% of the motile sperm that were found to have adherent particles of ASA- IgG. All the cases were found to be completely absent of ASA-IgA. Conclusion Endometriosis seems to have little impact on the production of circulating ASA. Clearly, ASA is not the key factor implicated in the pathogenesis of endometriosis-associated infertility. The presence of a low titer of ASA in a small proportion of the infertile women with endometriosis may exist by chance and, at least in part, explain the impaired fecundity in those patients.展开更多
To evaluate the level of antisperm antibody （ASA） in infertile men with oligoasthenoteratozoospermia （OAT）. Methods Forty-six infertile men with OAT were enrolled into this study. Sperm samples were screened by th...To evaluate the level of antisperm antibody （ASA） in infertile men with oligoasthenoteratozoospermia （OAT）. Methods Forty-six infertile men with OAT were enrolled into this study. Sperm samples were screened by the direct immunobead test for ASA type IgG and lgA according to the WHO laboratory manual. Results Of the 46 patients with OAT assessed for ASA-IgG, 2 had immunobead binding which were 22% and 27%, respectively, and the sub-positive rate was 4.3%. No case had the clinical positive level according to the WHO criteria （ ≥ 50% of the motile sperm with immunobead binding）. ASA-IgA was not detected in all cases. Conclusion A significant incidence or high level of ASA could not be found in infertile men with OAT, which suggests that ASA is not associated with the pathogenesis of infertile men with OAT.展开更多
Objective To evaluate levels of the circulating antisperm antibody （ASA） in women with secondary infertility. Methods Eighty-three women defined as secondary infertility were enrolled into this study. Based on their...Objective To evaluate levels of the circulating antisperm antibody （ASA） in women with secondary infertility. Methods Eighty-three women defined as secondary infertility were enrolled into this study. Based on their outcomes of previous pregnancy, these women were divided into two groups： group HC （having at least one child, n=45）, group NC （no child but one to three abortions, n=35）. The serum was screened by the indirect immunobead test for ASA type IgG and IgA according to the WHO laboratory manual. Results Of the 83 patients assessed for ASA-IgG, 14 had immunobead binding rate ranged from 15% to 43%, and the sub-positive rate was 16.9%. Five cases showed ASA-IgA sub-positive, and had binding rate between 10% and 20%. No case had the clinical positive level according to the WHO criteria. No differences were found on ASA-IgG and ASA-IgA between HC and NC groups （P〉0.05）. Conclusion The circulating ASA may be a possible cause to decrease fertility in women with secondary infertility.展开更多
Objective To investigate whether hemospermia is involved in the production of antisperm antibodies （ASA）. Methods A total of 40 males with hemospermia were enrolled in this study. These males were divided into 3 gro...Objective To investigate whether hemospermia is involved in the production of antisperm antibodies （ASA）. Methods A total of 40 males with hemospermia were enrolled in this study. These males were divided into 3 groups according to their fertile status： 18 cases were patients who visited the infertility clinic （group A）, 8 had fertility （group B）, and 14 did not know their fertile status （group C）. Semen samples were collected and ASA levels were detected by the direct immunobead test （D-IBT）. Leukocytes in semen were assessed by the peroxi- dase assay. Results None of these males with hemospermia were found to display significant levels of ASA in the 3 groups. The number of cases who had increased levels of leukocytes in the semen samples of groups A, B and C were 15 （83.3%）, 6 （75.0%） and 12 （85.7%）, respectively. Conclusion Hemospermia is not associated with the production of ASA. It is worth noting that the leukocyte levels are increased in semen of males with hemospermia.展开更多
Objective To evaluate the level of antisperm antibody （ASA） in infertile women with chronic salpingtis. Methods Forty-eight infertile women with chronic salpingtis were enrolled into this study. Serum samples were s...Objective To evaluate the level of antisperm antibody （ASA） in infertile women with chronic salpingtis. Methods Forty-eight infertile women with chronic salpingtis were enrolled into this study. Serum samples were screened by the direct immunobead test for ASA type IgG and IgA according to the WHO laboratory manual. About 50% or more of the motile sperm attaching to one or more immunobeads were regarded as clinical positivity according to the WHO criteria. About 20% 50% motile sperm that had adherent particles were deemed to be sub-positive. Results Of the 48 patients with chronic salpingtis assessed for ASA-IgG, 4 had immunobead bindings ranged from 50% to 60%, and the positive rate was 8.3%. In addition, 5 cases had 20%-40% of immunobead bindings, and the sub-positive rate was 10.4%. For ASA-IgA detecting, 2 cases with ASA-IgG positivity also had ASA-IgA immunobead bindings, and the positive rates were 22%, and 28%, respectively. Conclusion The incidence of ASA could be found in infertile women with chronic salpingtis, which suggests that an increased risk for the production of ASA would exist in the inflammatory status o f fallopian tube.展开更多
文摘Objective To determine the role of the circulating antisperm antibody （ASA） in the pathogenesis of missed abortion at the first-trimester pregnancy. Methods Sixty-two patients with a history of missed abortion at the first-trimester pregnancy were enrolled into this study. Indirect immunobead test （IBT） was used to measure the circulating ASA levels. Fifty healthy women with the first-trimester pregnancy set as the control. Results No case had the positive level of ASA according to the World Health Organization criteria （50% or more of the motile sperm with immunobead binding）. Only 1 case in patient group and 1 case in the control had 10%-20% of the motile spermatozoa with ASA-IgG bead binding. In both patient and control groups, ASA-IgA was found to be completely negative binding. Conclusion The circulating ASA is not associated with the pathogenesis of missed abortions at the first-trimester pregnancy.
文摘Objective To evaluate levels of the circulating antisperm antibody （ASA） in infertile women with polycystic ovary syndrome （PCOS）.Methods Forty-six infertile women with PCOS were enrolled into this study. The serum was screened by the indirect immunobead test for ASA type IgG and IgA according to the WHO laboratory manual.Results Of the 46 patients assessed for ASA-IgG, 3 cases had immunobead binding which were 20%, 27% and 35%, respectively, and the sub-positive rate was 6.5%. No case had the clinical positive level according to the WHO criteria （50% or more of the motile sperm with immunobead binding）. ASA-IgA was not detected in all cases. Conclusion The circulating ASA is not associated with the pathogenesis of infertile women with PCOS.
文摘Objective To investigate the role of circulating antisperm antibody （ASA） in the pathogenesis of endometriosis-associated infertility. Methods Serum samples were collected from 75 infertile women with minimal or mild endometriosis. Indirect immunobead test reporting on the percentage of motile sperm with adherent immunobeads was used to detect the circulating ASA levels. Results No infertile cases enrolled in the present study developed significant ASA level in the serum samples. That is to say, no one could achieve the diagnosis of ASAmediated infertility according to the World Health Organization criteria （50% or more of the motile sperm with immunobead binding）. There were only 5 cases （6.7%） who had 20%-40% of the motile sperm that were found to have adherent particles of ASA- IgG. All the cases were found to be completely absent of ASA-IgA. Conclusion Endometriosis seems to have little impact on the production of circulating ASA. Clearly, ASA is not the key factor implicated in the pathogenesis of endometriosis-associated infertility. The presence of a low titer of ASA in a small proportion of the infertile women with endometriosis may exist by chance and, at least in part, explain the impaired fecundity in those patients.
文摘To evaluate the level of antisperm antibody （ASA） in infertile men with oligoasthenoteratozoospermia （OAT）. Methods Forty-six infertile men with OAT were enrolled into this study. Sperm samples were screened by the direct immunobead test for ASA type IgG and lgA according to the WHO laboratory manual. Results Of the 46 patients with OAT assessed for ASA-IgG, 2 had immunobead binding which were 22% and 27%, respectively, and the sub-positive rate was 4.3%. No case had the clinical positive level according to the WHO criteria （ ≥ 50% of the motile sperm with immunobead binding）. ASA-IgA was not detected in all cases. Conclusion A significant incidence or high level of ASA could not be found in infertile men with OAT, which suggests that ASA is not associated with the pathogenesis of infertile men with OAT.
文摘Objective To evaluate levels of the circulating antisperm antibody （ASA） in women with secondary infertility. Methods Eighty-three women defined as secondary infertility were enrolled into this study. Based on their outcomes of previous pregnancy, these women were divided into two groups： group HC （having at least one child, n=45）, group NC （no child but one to three abortions, n=35）. The serum was screened by the indirect immunobead test for ASA type IgG and IgA according to the WHO laboratory manual. Results Of the 83 patients assessed for ASA-IgG, 14 had immunobead binding rate ranged from 15% to 43%, and the sub-positive rate was 16.9%. Five cases showed ASA-IgA sub-positive, and had binding rate between 10% and 20%. No case had the clinical positive level according to the WHO criteria. No differences were found on ASA-IgG and ASA-IgA between HC and NC groups （P〉0.05）. Conclusion The circulating ASA may be a possible cause to decrease fertility in women with secondary infertility.
文摘Objective To investigate whether hemospermia is involved in the production of antisperm antibodies （ASA）. Methods A total of 40 males with hemospermia were enrolled in this study. These males were divided into 3 groups according to their fertile status： 18 cases were patients who visited the infertility clinic （group A）, 8 had fertility （group B）, and 14 did not know their fertile status （group C）. Semen samples were collected and ASA levels were detected by the direct immunobead test （D-IBT）. Leukocytes in semen were assessed by the peroxi- dase assay. Results None of these males with hemospermia were found to display significant levels of ASA in the 3 groups. The number of cases who had increased levels of leukocytes in the semen samples of groups A, B and C were 15 （83.3%）, 6 （75.0%） and 12 （85.7%）, respectively. Conclusion Hemospermia is not associated with the production of ASA. It is worth noting that the leukocyte levels are increased in semen of males with hemospermia.
文摘Objective To evaluate the level of antisperm antibody （ASA） in infertile women with chronic salpingtis. Methods Forty-eight infertile women with chronic salpingtis were enrolled into this study. Serum samples were screened by the direct immunobead test for ASA type IgG and IgA according to the WHO laboratory manual. About 50% or more of the motile sperm attaching to one or more immunobeads were regarded as clinical positivity according to the WHO criteria. About 20% 50% motile sperm that had adherent particles were deemed to be sub-positive. Results Of the 48 patients with chronic salpingtis assessed for ASA-IgG, 4 had immunobead bindings ranged from 50% to 60%, and the positive rate was 8.3%. In addition, 5 cases had 20%-40% of immunobead bindings, and the sub-positive rate was 10.4%. For ASA-IgA detecting, 2 cases with ASA-IgG positivity also had ASA-IgA immunobead bindings, and the positive rates were 22%, and 28%, respectively. Conclusion The incidence of ASA could be found in infertile women with chronic salpingtis, which suggests that an increased risk for the production of ASA would exist in the inflammatory status o f fallopian tube.
