The gene of trichosanthin has been transferred into rice plants through agrobacterium method. The single copy insertion and the expression of foreign gene have been proved in regenerated plants. In antifungal assay th...The gene of trichosanthin has been transferred into rice plants through agrobacterium method. The single copy insertion and the expression of foreign gene have been proved in regenerated plants. In antifungal assay the degrees of rice blast (Pyricularia oryzae) infection of the transgenic plants expressing trichosanthin and expressing GUS gene as control have been evaluated. The differences such as the time of disease symptom observed, the number of infected plants and damaged leaves, the growth of infected plants of the two transgenic plants after being inoculated by rice blast (Pyricularia oryzae) are significant. The transgenic plants with trichosanthin gene grew faster than the plants with GUS gene, even when humidity environment was removed. The results show that the transgenic plants that expressed trichosanthin are able to delay the infection of rice blast compared with the plants as control. In addition, no damage caused by the expression of trichosanthin gene in transgenic plants has been展开更多
Crude of Plant extracts from materials is necessary screening to determine new biological activities, toxicity and active compounds in bioassay tests. Crude of Populus euphratica L. left is tested to phytochemical scr...Crude of Plant extracts from materials is necessary screening to determine new biological activities, toxicity and active compounds in bioassay tests. Crude of Populus euphratica L. left is tested to phytochemical screening, constituents, fractionation and MIC technique as anti-fungal. The fractions are subjected to bio-compound analysis using Liquid chromatography-mass spectrometry LC-MS. Phytochemical screening of crude extract shows presence of Glycosides compared with other compounds. While the fraction of ethanolic extract recorded inhibition impact reached (390, mg/L/MIC) agnist type of fungi. Bioassay toxicity using shrimp lethally test (LC50) was carried out with the result showing 4.1 mg/L is low toxic and can be used safely. Alpha Glycoside (4, 6 Benzylidene-1O-Methyl-2O-(2346 Tetra-O-Acetyl-Betad- Glucosyl), is a new compound and antifungal action.展开更多
文摘The gene of trichosanthin has been transferred into rice plants through agrobacterium method. The single copy insertion and the expression of foreign gene have been proved in regenerated plants. In antifungal assay the degrees of rice blast (Pyricularia oryzae) infection of the transgenic plants expressing trichosanthin and expressing GUS gene as control have been evaluated. The differences such as the time of disease symptom observed, the number of infected plants and damaged leaves, the growth of infected plants of the two transgenic plants after being inoculated by rice blast (Pyricularia oryzae) are significant. The transgenic plants with trichosanthin gene grew faster than the plants with GUS gene, even when humidity environment was removed. The results show that the transgenic plants that expressed trichosanthin are able to delay the infection of rice blast compared with the plants as control. In addition, no damage caused by the expression of trichosanthin gene in transgenic plants has been
文摘Crude of Plant extracts from materials is necessary screening to determine new biological activities, toxicity and active compounds in bioassay tests. Crude of Populus euphratica L. left is tested to phytochemical screening, constituents, fractionation and MIC technique as anti-fungal. The fractions are subjected to bio-compound analysis using Liquid chromatography-mass spectrometry LC-MS. Phytochemical screening of crude extract shows presence of Glycosides compared with other compounds. While the fraction of ethanolic extract recorded inhibition impact reached (390, mg/L/MIC) agnist type of fungi. Bioassay toxicity using shrimp lethally test (LC50) was carried out with the result showing 4.1 mg/L is low toxic and can be used safely. Alpha Glycoside (4, 6 Benzylidene-1O-Methyl-2O-(2346 Tetra-O-Acetyl-Betad- Glucosyl), is a new compound and antifungal action.
