以耐冷性存在明显差异的栽培黄瓜‘长春密刺’和‘北京截头’为材料,研究了冷胁迫下C a2+、钙离子螯合剂(EGTA)和钙调素拮抗剂(CPZ)对低温胁迫黄瓜幼苗叶片3种抗氧化酶活性及POD同工酶的影响.对保护酶活性的研究结果表明:C aC l2、EGTA...以耐冷性存在明显差异的栽培黄瓜‘长春密刺’和‘北京截头’为材料,研究了冷胁迫下C a2+、钙离子螯合剂(EGTA)和钙调素拮抗剂(CPZ)对低温胁迫黄瓜幼苗叶片3种抗氧化酶活性及POD同工酶的影响.对保护酶活性的研究结果表明:C aC l2、EGTA和CPZ处理均能引起3种抗氧化酶活性变化,但变化幅度因材料而异.胁迫处理中C a2+能提高2种材料的叶片抗氧化酶活性,减少其在低温逆境中的下降幅度,而EGTA和CPZ处理降低了2种材料的抗氧化酶活性,加大了低温逆境中的下降幅度.POD同工酶研究发现,C aC l2延缓冷胁迫中同工酶条带的丢失,EGTA和CPZ处理则使同工酶条带丢失提前,但材料不同对酶条带丢失时间也有影响.展开更多
The experiment was carried out to study the genotypic difference in the responses of seed germination, growth and physiological characters of rice seedlings to Cd toxicity. The result showed that the germination was s...The experiment was carried out to study the genotypic difference in the responses of seed germination, growth and physiological characters of rice seedlings to Cd toxicity. The result showed that the germination was slightly stimulated under low Cd concentration （0.01-1.5 mM Cd）, while severely depressed under higher Cd concentration （2.0 mM）. Rice seedlings exposed to 0.01 mM Cd showed slight increases in plant height, root volume, biomass and chlorophyll concentration. These parameters were significantly reduced when Cd level in the medium was increased to 0.5 mM, and meanwhile corresponding increase in superoxide dismutase （SOD） and peroxidase （POD） activities, and MDA （malondialdehyde） content was observed. However, SOD and POD activities declined when plants were exposed to 1 mM Cd when compared with those under 0.5 mM Cd. Cadmium addition lowered Fe, Cu and Mn concentrations in roots and shoots. There was significant genotypic difference in the response of these parameters to Cd stress. Under Cd stress, Xiushui 110 had the least inhibition of growth and increase in MDA content, higher shoot Cd concentration, and greatest increase in POD and SOD activities, indicating its higher tolerance to Cd toxicity, while Bing 9914 had the greatest reduction of growth, and Zn, Cu, Fe, and Mn contents, but greatest increase in MDA content, and least increase in activities of antioxidative enzymes, indicating its sensitivity to Cd toxicity.展开更多
采用生物量测定、3,5-二硝基水杨酸比色法、对-棕榈酸硝基苯酯(ρ-NPP)比色法、福林-酚法等方法研究不同养殖密度和换水频率对蚂蟥生长、消化酶活性、生化指标和内在品质的影响。结果表明:蚂蟥的最终质量,特定生长率(SGR),增重率(WGR),...采用生物量测定、3,5-二硝基水杨酸比色法、对-棕榈酸硝基苯酯(ρ-NPP)比色法、福林-酚法等方法研究不同养殖密度和换水频率对蚂蟥生长、消化酶活性、生化指标和内在品质的影响。结果表明:蚂蟥的最终质量,特定生长率(SGR),增重率(WGR),淀粉酶,脂肪酶,蛋白酶活力,SOD,CAT,ALP酶活性与密度呈反比,与换水频率呈正比(P<0.05);换水频率与水中氨氮、亚硝酸盐和硫化氢呈负相关,与溶氧量呈正相关;养殖密度和换水频率对蚂蟥水分、总灰分、酸不溶性灰分、p H和抗凝血酶活性影响不显著。因此,每亩50万条为较适宜的养殖密度,每72 h 1次为较适宜的换水频率。展开更多
文摘以耐冷性存在明显差异的栽培黄瓜‘长春密刺’和‘北京截头’为材料,研究了冷胁迫下C a2+、钙离子螯合剂(EGTA)和钙调素拮抗剂(CPZ)对低温胁迫黄瓜幼苗叶片3种抗氧化酶活性及POD同工酶的影响.对保护酶活性的研究结果表明:C aC l2、EGTA和CPZ处理均能引起3种抗氧化酶活性变化,但变化幅度因材料而异.胁迫处理中C a2+能提高2种材料的叶片抗氧化酶活性,减少其在低温逆境中的下降幅度,而EGTA和CPZ处理降低了2种材料的抗氧化酶活性,加大了低温逆境中的下降幅度.POD同工酶研究发现,C aC l2延缓冷胁迫中同工酶条带的丢失,EGTA和CPZ处理则使同工酶条带丢失提前,但材料不同对酶条带丢失时间也有影响.
基金the Key Program of Zhejiang Natural Science Foundation,China(Z304104)for its support for this project.
文摘The experiment was carried out to study the genotypic difference in the responses of seed germination, growth and physiological characters of rice seedlings to Cd toxicity. The result showed that the germination was slightly stimulated under low Cd concentration （0.01-1.5 mM Cd）, while severely depressed under higher Cd concentration （2.0 mM）. Rice seedlings exposed to 0.01 mM Cd showed slight increases in plant height, root volume, biomass and chlorophyll concentration. These parameters were significantly reduced when Cd level in the medium was increased to 0.5 mM, and meanwhile corresponding increase in superoxide dismutase （SOD） and peroxidase （POD） activities, and MDA （malondialdehyde） content was observed. However, SOD and POD activities declined when plants were exposed to 1 mM Cd when compared with those under 0.5 mM Cd. Cadmium addition lowered Fe, Cu and Mn concentrations in roots and shoots. There was significant genotypic difference in the response of these parameters to Cd stress. Under Cd stress, Xiushui 110 had the least inhibition of growth and increase in MDA content, higher shoot Cd concentration, and greatest increase in POD and SOD activities, indicating its higher tolerance to Cd toxicity, while Bing 9914 had the greatest reduction of growth, and Zn, Cu, Fe, and Mn contents, but greatest increase in MDA content, and least increase in activities of antioxidative enzymes, indicating its sensitivity to Cd toxicity.
文摘采用生物量测定、3,5-二硝基水杨酸比色法、对-棕榈酸硝基苯酯(ρ-NPP)比色法、福林-酚法等方法研究不同养殖密度和换水频率对蚂蟥生长、消化酶活性、生化指标和内在品质的影响。结果表明:蚂蟥的最终质量,特定生长率(SGR),增重率(WGR),淀粉酶,脂肪酶,蛋白酶活力,SOD,CAT,ALP酶活性与密度呈反比,与换水频率呈正比(P<0.05);换水频率与水中氨氮、亚硝酸盐和硫化氢呈负相关,与溶氧量呈正相关;养殖密度和换水频率对蚂蟥水分、总灰分、酸不溶性灰分、p H和抗凝血酶活性影响不显著。因此,每亩50万条为较适宜的养殖密度,每72 h 1次为较适宜的换水频率。
