This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture te...This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body （PLB） formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid （2,4-D） and 8.88μmol L^-1 N6-benzyladenine （BA）. The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.展开更多
[Objective] The research aimed to study the vase preservative of Anthurium seherzerianum cut flowers and provide reference for the planting and preservative method selection of A.seherzerianum.[Method] Using Tropical...[Objective] The research aimed to study the vase preservative of Anthurium seherzerianum cut flowers and provide reference for the planting and preservative method selection of A.seherzerianum.[Method] Using Tropical＇s spathe,one kind of common cut blossoms of A.seherzerianum as experimental materials,three different formula of vase solutions were used.And it was compared with common preservative formula of A.seherzerianum to study the vase preserved liquid of A.seherzerianum.[Result] Formula 3 was the optimum vase preservative,which was composed of 4% sucrose,0.08% NaCl,0.01% Ca（H2PO4）2·H2O,0.01% Chinese medicine antiseptic （ethonal extract from coptis）,0.1 mol/L NaOH,0.1 mol/L citric acid and 10 mg/L 6-BA.The preserved period reached 23 days,13 days longer than CK.The appreciative period reached 31 days,18 days longer than CK,with significant effects.In formula 3,water loss in A.seherzerianum spathe was decreased obviously,which was favorable for maintaining water content in tissues.The cell membrane permeability was reduced and the peroxidation of membrane ester was inhibited.The accumulation of MDA was decreased and SOD activity was increased.The protective enzyme activity of cells was enhanced and the content of proline and soluble sugar were increased and the respiration rate was reduced.[Conclusion] The formula（4% sucrose ＋ 0.08% NaCl＋ 0.01% Ca（H2PO4）2＋0.01% Chinese medicine antiseptic（ethonal extract from coptis）＋ 0.1 mol/L NaOH＋0.1 mol/L citric acid and 10 mg/L 6-BA）achieved the purpose of prolonging the preservative period of A.seherzerianum spathe and prolonging the preservative period of A.seherzerianum effectively.展开更多
基金supported by the Natural Science Foundation of Guangdong Province (05300848)Fok Ying Tung Education Foundation (104031)the National Natural Science Foundation of China(30800758)
文摘This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body （PLB） formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid （2,4-D） and 8.88μmol L^-1 N6-benzyladenine （BA）. The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.
基金Supported by National Science and Technology Supporting Project(2007BAD45B09-02)~~
文摘[Objective] The research aimed to study the vase preservative of Anthurium seherzerianum cut flowers and provide reference for the planting and preservative method selection of A.seherzerianum.[Method] Using Tropical＇s spathe,one kind of common cut blossoms of A.seherzerianum as experimental materials,three different formula of vase solutions were used.And it was compared with common preservative formula of A.seherzerianum to study the vase preserved liquid of A.seherzerianum.[Result] Formula 3 was the optimum vase preservative,which was composed of 4% sucrose,0.08% NaCl,0.01% Ca（H2PO4）2·H2O,0.01% Chinese medicine antiseptic （ethonal extract from coptis）,0.1 mol/L NaOH,0.1 mol/L citric acid and 10 mg/L 6-BA.The preserved period reached 23 days,13 days longer than CK.The appreciative period reached 31 days,18 days longer than CK,with significant effects.In formula 3,water loss in A.seherzerianum spathe was decreased obviously,which was favorable for maintaining water content in tissues.The cell membrane permeability was reduced and the peroxidation of membrane ester was inhibited.The accumulation of MDA was decreased and SOD activity was increased.The protective enzyme activity of cells was enhanced and the content of proline and soluble sugar were increased and the respiration rate was reduced.[Conclusion] The formula（4% sucrose ＋ 0.08% NaCl＋ 0.01% Ca（H2PO4）2＋0.01% Chinese medicine antiseptic（ethonal extract from coptis）＋ 0.1 mol/L NaOH＋0.1 mol/L citric acid and 10 mg/L 6-BA）achieved the purpose of prolonging the preservative period of A.seherzerianum spathe and prolonging the preservative period of A.seherzerianum effectively.
