期刊文献+
共找到37篇文章
< 1 2 >
每页显示 20 50 100
溶胶-凝胶生物传感器 被引量:17
1
作者 王炳全 程广金 董绍俊 《分析化学》 SCIE EI CAS CSCD 北大核心 1999年第8期982-988,共7页
评述了溶胶-凝胶(sol-gel)生物传感器这个领域已取得的成果,主要内容涉及溶胶-凝胶固定化方法的过程、优点与性质,溶胶-凝胶光学生物传感器和安培生物传感器的发展,并展望了其发展趋势.
关键词 溶胶 凝胶 光学 生物传感器 安培 评述
下载PDF
基于氧化还原聚合物固定双酶的谷氨酸传感器的研究 被引量:9
2
作者 李华清 郭宗慧 +5 位作者 刘春秀 姜利英 崔大付 刘文勇 黄勇 蔡新霞 《分析化学》 SCIE EI CAS CSCD 北大核心 2006年第U09期1-4,共4页
在塑料基片上制备了金薄膜的两电极系统的生物传感器。集成化的Ag|AgCl参比电极采用丝网印刷在薄膜电极上。以聚乙二醇二缩水甘油醚(PEGDGE)为交联剂,将辣根过氧化酶(HRP)和谷氨酸氧化酶(GLOD)固定到聚乙烯吡啶与2,2'-双吡啶... 在塑料基片上制备了金薄膜的两电极系统的生物传感器。集成化的Ag|AgCl参比电极采用丝网印刷在薄膜电极上。以聚乙二醇二缩水甘油醚(PEGDGE)为交联剂,将辣根过氧化酶(HRP)和谷氨酸氧化酶(GLOD)固定到聚乙烯吡啶与2,2'-双吡啶锇形成的氧化还原复合物中,滴在电极表面形成传感器的工作电极。实验结果表明,该生物传感器在谷氨酸溶液的浓度为1.0×10^-6~4.0×10^-4moL/L的范围内,具有很好的线性关系(r=0.9998,n=9),灵敏度为37.5mA(moL/L)^-1cm^-2;谷氨酸检出限为1.0×10^-6mol/L;传感器响应时间为10s且有良好的一致性和稳定性,使用一个月后仍能保持其初始活性的90%。该传感器可以用于食品工业中的谷氨酸的快速检测和在线监测等。 展开更多
关键词 氧化还原聚合物 电流型生物传感器 谷氨酸氧化酶 辣根过氧化酶
下载PDF
基于酶的电化学生物传感器在食品检测中应用的研究进展 被引量:6
3
作者 饶钧玥 吴任之 +2 位作者 曹芸榕 杨茂杰 韩国全 《食品与发酵工业》 CAS CSCD 北大核心 2022年第18期337-344,共8页
开发快速、准确定量监测加工过程中生成的代谢产物并提供实时结果的技术,对保证食品质量安全具有重大意义。该文介绍以酶为识别元件的3种电化学生物传感器(电流传感器、电导传感器、电位传感器)在葡萄糖、酪胺和真菌毒素等检测应用中的... 开发快速、准确定量监测加工过程中生成的代谢产物并提供实时结果的技术,对保证食品质量安全具有重大意义。该文介绍以酶为识别元件的3种电化学生物传感器(电流传感器、电导传感器、电位传感器)在葡萄糖、酪胺和真菌毒素等检测应用中的优势及特点,以期为食品加工过程监测的选择及研发提供思路。 展开更多
关键词 固定化酶 电流生物传感器 电导生物传感器 电位生物传感器 食品检测
下载PDF
Amperometric Biosensor for Hydrogen Peroxide Based on Electrodeposited Sub-micrometer Gold Modified Glassy Carbon Electrode
4
作者 王树青 陈峻 林祥钦 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2004年第4期360-364,共5页
A new type of hydrogen peroxide amperometric biosensor was fabricated based on electrochemically deposited sub-micrometer Au particles (sm-Au) on a glassy carbon electrode (GCE). Electrochemical deposition condition w... A new type of hydrogen peroxide amperometric biosensor was fabricated based on electrochemically deposited sub-micrometer Au particles (sm-Au) on a glassy carbon electrode (GCE). Electrochemical deposition condition was optimized for obtaining uniformly distributed sub-micrometer sized Au array on the electrode surface. The hy-drogen peroxide sensor was fabricated by adsorbing phenothiazine methylene blue (MB) molecules on the surface of sm-Au and covering a cross-linked horseradish peroxidase (HRP) layer, labeled as HRP/MB/sm-Au/GCE. The characteristics of this biosensor were evaluated with respect to applied potential and pH. The amperometric re-sponse of the sensor was linear to the H2O2 concentration over a wide range of 9.9×10-61.11×10-2 mol/L. A detection limit (s/n=3) of 3.0×10-6 mol/L H2O2 was estimated for a sampled chronoamperometric detection at 1.5 min after potential step of 200 to -400 mV vs. SCE. The immobilized MB molecules shuttled electrons at a=0.77 and an apparent electron transfer rate constant of 0'sk=0.053 s-1. Interference of ascorbic acid, dopamine and uric acid was investigated. This sensor has very good stability and reproducibility for long-term use. 展开更多
关键词 hydrogen peroxide amperometric biosensor sub-micrometer gold glassy carbon electrode me-thylene blue horseradish peroxidase
原文传递
检测有机磷农药电流型胆碱酯酶传感器的研究进展 被引量:3
5
作者 张淑平 单联刚 经媛元 《化学传感器》 CAS 2006年第2期8-13,共6页
综述了检测有机磷农药电流型胆碱酯酶传感器的研究概况,对胆碱酯酶的固定化技术,工作电极的种类以及电化学修饰进行了探讨,并就其发展前景进行了展望。
关键词 电流型生物传感器 乙酰胆碱酯酶 生物传感器 工作电极 酶固定化 化学修饰电极
下载PDF
基于溶胶-凝胶技术结合多壁纳米碳管化学修饰电极的方法制备高灵敏葡萄糖生物传感器的研究 被引量:5
6
作者 黄加栋 宋昭 +4 位作者 杨钰 吴宝艳 史海滨 长哲郎 陈强 《高技术通讯》 CAS CSCD 北大核心 2006年第5期492-496,共5页
基于多壁纳米碳管修饰铂电极与二氧化硅溶胶-凝胶(sol-gel)固定化酶相结合的技术制备了葡萄糖氧化酶传感器,充分利用了溶胶-凝胶固定化酶稳定的优点和纳米碳管的高灵敏电催化作用,优化了该酶传感器的制备过程,提高了传感器的电流响应... 基于多壁纳米碳管修饰铂电极与二氧化硅溶胶-凝胶(sol-gel)固定化酶相结合的技术制备了葡萄糖氧化酶传感器,充分利用了溶胶-凝胶固定化酶稳定的优点和纳米碳管的高灵敏电催化作用,优化了该酶传感器的制备过程,提高了传感器的电流响应和反应线性.结果表明,sol-gel构建的优化条件是:H2O:TEOS为2.5~3.5,TritonX-100浓度为5%,pH值为5.5.在本实验条件下,多壁纳米碳管的最适固定量为5μl(0.25g/L),溶胶-凝胶与酶的优化体积比为3:2.工作电位+0.55V、pH 6.5、25℃为制备传感器的最适工作条件.该传感器对葡萄糖在0.5~6 mmol/L呈线性响应,响应时间为20 s,检出限为0.05mmol/L,45天时的响应值仍保持90%. 展开更多
关键词 溶胶-凝胶 多壁纳米碳管 化学修饰电极 葡萄糖氧化酶 电流型生物传感器
下载PDF
基于Fe3O4-PGA@Au构建无酶电化学生物传感器检测葡萄糖 被引量:5
7
作者 关桦楠 龚德状 +5 位作者 宋岩 刘博 韩博林 杨帆 崔琳琳 张娜 《食品科学》 EI CAS CSCD 北大核心 2020年第12期267-272,共6页
基于聚谷氨酸(poly-(γ-glutamic acid),PGA)金磁微粒(Fe3O4-PGA@Au)电沉积修饰玻碳电极,构建具有高灵敏度和选择性的无酶型葡萄糖电化学生物传感器。采用绿色还原吸附法制备Fe3O4-PGA@Au,并利用X射线衍射、傅里叶变换红外光谱和透射电... 基于聚谷氨酸(poly-(γ-glutamic acid),PGA)金磁微粒(Fe3O4-PGA@Au)电沉积修饰玻碳电极,构建具有高灵敏度和选择性的无酶型葡萄糖电化学生物传感器。采用绿色还原吸附法制备Fe3O4-PGA@Au,并利用X射线衍射、傅里叶变换红外光谱和透射电子显微镜表征Fe3O4-PGA@Au的理化性质。优化葡萄糖传感检测体系的反应温度、pH值、扫描速率和反应时间等检测参数。采用循环伏安法考察无酶传感器检测葡萄糖时的电化学行为。结果表明,Fe3O4-PGA@Au具有良好的电催化性能;在葡萄糖浓度分别为0.1~5.0μmol/L和10~250μmol/L范围内时,所构建的生物传感器峰电流密度与其葡萄糖浓度具有良好的线性关系,检出限为0.74μmol/L(RSN=3);此无酶型生物传感器具有好的重复性和抗干扰性能。 展开更多
关键词 葡萄糖 电化学生物传感器 纳米模拟酶 金纳米粒子 四氧化三铁 聚谷氨酸
下载PDF
氧化石墨烯-硫堇及纳米金修饰玻碳电极电流型葡萄糖生物传感器的研究 被引量:4
8
作者 李岩 唐点平 《福州大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第5期781-785,共5页
以玻碳电极为基底,在电极表面修饰一层氧化石墨烯-硫堇(GO-Th)薄膜,通过层层自组装方式,将纳米金和葡萄糖氧化酶(GOD)固定在玻碳电极表面,制得一种新型电流型葡萄糖生物传感器.在优化的实验条件下,该生物传感器对葡萄糖的线性响应范围为... 以玻碳电极为基底,在电极表面修饰一层氧化石墨烯-硫堇(GO-Th)薄膜,通过层层自组装方式,将纳米金和葡萄糖氧化酶(GOD)固定在玻碳电极表面,制得一种新型电流型葡萄糖生物传感器.在优化的实验条件下,该生物传感器对葡萄糖的线性响应范围为1.0×10-9~5.7×10-5 mol.L-1,检测下限为5.0×10-10mol.L-1.该传感器具有制备方法简单、灵敏度高、稳定性好等特点. 展开更多
关键词 葡萄糖 电流型生物传感器 纳米金溶胶 氧化石墨烯 硫堇
原文传递
Determination of mercury and nickel by amperometric biosensor prepared with thermostable lactate dehydrogenase 被引量:3
9
作者 brahim TAN Elif ERHAN +1 位作者 Pinar KARAGZ Melek ZKAN 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2011年第10期2332-2338,共7页
Response of biosensor prepared with the thermostable bacterial LDH enzyme was analyzed in the presence of mercury and nickel.For electrode preparation,the enzyme was purified and immobilized on a gold sheet coated by ... Response of biosensor prepared with the thermostable bacterial LDH enzyme was analyzed in the presence of mercury and nickel.For electrode preparation,the enzyme was purified and immobilized on a gold sheet coated by PGA-pyrrole polymeric material.The working electrode was tested at increasing concentration of lactate in the presence of two different concentrations of mercury and nickel.Current response of biosensor decreased from 0.32 μA to 0.09 μA and 4.13 μA to 2.63 μA when 25×10-7 mmol/L mercury and 17×10-5 mmol/L nickel were included in the working solution,respectively.Sensitivity of the electrode decreased from 0.010 2 μA/(mmol·L-1) to 0.0043 μA/(mmol·L-1) in the presence of 25×10-7 mmol/L mercury.On the other hand,the presence of nickel did not result in a decrease in electrode sensitivity.The results pointed out that the prepared biosensor is useful to detect mercury in a sample containing both mercury and nickel together. 展开更多
关键词 enzyme inhibition amperometric biosensor heavy metals
下载PDF
氧化还原聚合物修饰的过氧化氢传感器的研究 被引量:1
10
作者 李华清 刘春秀 +3 位作者 郭宗慧 姜利英 崔大付 蔡新霞 《传感技术学报》 EI CAS CSCD 北大核心 2006年第3期549-551,共3页
一种用于快速检测过氧化氢的电流型生物传感器。将氧化还原聚合物滴在塑料基片上溅射的金薄膜工作电极上形成一层对过氧化氢的敏感膜。实验结果表明,该生物传感器在1.0×10-6-2.0×10-4mol,具有很好的线性相关,线性相关系数为0.... 一种用于快速检测过氧化氢的电流型生物传感器。将氧化还原聚合物滴在塑料基片上溅射的金薄膜工作电极上形成一层对过氧化氢的敏感膜。实验结果表明,该生物传感器在1.0×10-6-2.0×10-4mol,具有很好的线性相关,线性相关系数为0.999(n=9),灵敏度为0.6A/(mol·cm2),检出限为1μMH2O2,稳定性好且批量制备的传感器之间一致性好([CV]=4.2%),因此,该方法能够用于批量制备低成本的生物传感器。 展开更多
关键词 氧化还原聚合物 电子媒介体 过氧化氢 生物传感器
下载PDF
Analytical Solution of Substrate Concentration in the Biosensor Response
11
作者 Seyed Ali Madani Tonekaboni Ali Shahbazi Mastan Abad +3 位作者 Amin Afshari Ali Khalilzadeh Shahab Karimi Mitra Shabanisamghabady 《Applied Mathematics》 2013年第12期1603-1608,共6页
Homotopy analysis method (HAM) is employed to investigate amperometric biosensor at mixed enzyme kinetics and diffusion limitation. Mathematical modeling of the problem is developed utilizing non-Michaelis-Menten kine... Homotopy analysis method (HAM) is employed to investigate amperometric biosensor at mixed enzyme kinetics and diffusion limitation. Mathematical modeling of the problem is developed utilizing non-Michaelis-Menten kinetics of the enzymatic reaction. Different results of the problem are obtained for different values of the dimensionless parameters. Accuracy of the obtained results is verified by comparing them with the available actual and simulated ones. It is concluded that the obtained solution can be considered as a promising one to investigate different aspects of the phenomena. 展开更多
关键词 HOMOTOPY Analysis Method amperometric biosensor MATHEMATICAL Modeling Non-Michaelis-Menten Kinetics
下载PDF
A New Amperometric Biosensor Based on HRP/Nano-Au/L-Cysteine/Poly(o-Aminobenzoic acid)-Membrane-Modified Platinum Electrode for the Determination of Hydrogen Peroxide
12
作者 唐明宇 袁若 柴雅琴 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2006年第11期1575-1580,共6页
The third generation amperometric biosensor for the determination of hydrogen peroxide (H2O2) has been described. For the fabrication of biosensor, o-aminobenzoic acid (oABA) was first electropolymerized on the su... The third generation amperometric biosensor for the determination of hydrogen peroxide (H2O2) has been described. For the fabrication of biosensor, o-aminobenzoic acid (oABA) was first electropolymerized on the surface of platinum (Pt) electrode as an electrostatic repulsion layer to reject interferences. Horseradish peroxidase (HRP) absorbed by nano-scaled particulate gold (nano-Au) was immobilized on the electrode modified with polymerized o-aminobenzoic acid (poABA) with L-cysteine as a linker to prepare a biosensor for the detection of H2O2. Amperometric detection of H2O2 was realized at a potential of +20 mV versus SCE. The resulting biosensor exhibited fast response, excellent reproducibility and sensibility, expanded linear range and low interferences. Temperature and pH dependence and stability of the sensor were investigated. The optimal sensor gave a linear response in the range of 2.99×10^-6 to 3.55×10^-3 mol·L^-1 to H2O2 with a sensibility of 0.0177 A·L^-1·mol^-1 and a detection limit (S/N = 3) of 4.3×10^-7 mol·L^-1. The biosensor demonstrated a 95% response within less than 10 s. 展开更多
关键词 amperometric biosensor hydrogen peroxide poly(o-aminobenzoic acid) nano-Au horseradish peroxidase
原文传递
Non-Conducting Poly(O-Aminophenol) Films in the Field of the Bioelectrochemistry
13
作者 Ricardo Tucceri 《American Journal of Analytical Chemistry》 2013年第6期13-26,共14页
The practical use of non-conducting poly(o-aminophenol) (POAP) films in the field of the bioelectrochemistry is discussed in this paper. Particular emphasis is given to the effects of applied potential, solution pH an... The practical use of non-conducting poly(o-aminophenol) (POAP) films in the field of the bioelectrochemistry is discussed in this paper. Particular emphasis is given to the effects of applied potential, solution pH and interferents on the response current of biosensors based on POAP. 展开更多
关键词 Poly(O-Aminophenol) biosensor amperometric Response Glucose Uric Acid Hydrogen Peroxide Lactate NICOTINAMIDE COENZYMES
下载PDF
Amperometric Determination of Serum Cholesterol with Pencil Graphite Rod
14
作者 Nidhi Chauhan Jagriti Narang Chandra S. Pundir 《American Journal of Analytical Chemistry》 2010年第2期41-46,共6页
A cholesterol oxidase from Streptomycin sp. was immobilized onto pencil graphite rod and employed for amperometric determination of serum cholesterol. The method has the advantage over earlier amperometric methods tha... A cholesterol oxidase from Streptomycin sp. was immobilized onto pencil graphite rod and employed for amperometric determination of serum cholesterol. The method has the advantage over earlier amperometric methods that it requires low potential to generate electrons from H2O2, which does not allow ionization of serum substances. The optimum working conditions of amperometric determination were pH 6.8, 25?C and 30 s. The current measured was in proportion to cholesterol concentration ranging from 1.29×10-3 to 10.33×10-3 M. Minimum detection limit of the method was 0.09 ×10-3 M. Mean analytical recovery of added cholesterol (100 mg/dl and 200 mg/dl) in serum was 85.0% & 90.0% respectively. Within batch and between batch coefficients of variations were 1.59% & 4.15% respectively. A good correlation (r = 0.99) was obtained between serum cholesterol values by standard enzymic colorimetric method and the present method. No interference by metabolites was observed in the method. The enzyme electrode was reused 200 times over a period of 25 days, when stored at 4? 展开更多
关键词 CHOLESTEROL CHOLESTEROL OXIDASE PENCIL GRAPHITE Enzyme Electrode Serum amperometric biosensor
下载PDF
Development of Enzyme Biosensor for Amperometric Measurement of Aspartate Aminotransferase
15
作者 Zonghui Guo Chunxiu Liu +3 位作者 Huaqing Li Jianfang Xu Lianshi Feng Xinxia Cai 《稀有金属材料与工程》 SCIE EI CAS CSCD 北大核心 2006年第A03期330-332,共3页
An enzyme biosensor for amperometric measurement of aspartate aminotransferase has been developed.The working electrode was modified with a thin-film of redox polymer,then glutamate oxidase,with the immobilized reagen... An enzyme biosensor for amperometric measurement of aspartate aminotransferase has been developed.The working electrode was modified with a thin-film of redox polymer,then glutamate oxidase,with the immobilized reagent cast and dried on the electrode.The biosensor responses to AST by detecting hydrogen peroxide were produced by enzymical reaction at-0.1 V with a response time of 120 seconds.The electrode gave a detection limit of 32.5 U/L with a linear concentration range of 32.5 U/L~2000 U/L in serum.Due to more sensitive and lower detection limit,the biosensor is expected mainly to be used for physiological identification and physical performance of athletes in the future.Extended application will also affect the practice of clinical medicine for the diagnosis of heart and liver disease. 展开更多
关键词 aspartate aminotransferase redox polymer amperometric biosensor
下载PDF
Disposable Amperometric Acetylcholinesterase Biosensor for the Detection of Organophosphorus Pesticides
16
作者 Xiaohao Wang Xiaowen Xu Fei Tang Zhaoying Zhou 《稀有金属材料与工程》 SCIE EI CAS CSCD 北大核心 2006年第A03期333-336,共4页
A rapid,simple,disposable and inexpensive acetylcholinesterase (ACHE) amperometric biosensor for the detection of organophosphorus pesticides was developed by simple adsorption of the enzyme on screen-printed electrod... A rapid,simple,disposable and inexpensive acetylcholinesterase (ACHE) amperometric biosensor for the detection of organophosphorus pesticides was developed by simple adsorption of the enzyme on screen-printed electrodes.The biosensor consisted of an Ag/AgCl reference electrode and a graphite working electrode.The mixture of graphite and the 7,7,8,8-tetracyanoquinodimethane (TCNQ) was printed on electrodes.The detection of organophosphorus pesticides was done with acetylthiocholine chloride (ATCh) as substrate.The biosensor was used to detect the inhibitory effect of organophosphorus pesticides on AChE activity.The 1μl of enzyme solution containing 0.1 U AChE and 1% bovine serum albumin (BSA) were simply dropped on the working electrode surface.The biosensor operated at a potential of 300 mV vs. Ag/AgCl in a pH 7.2 0.1 mol/L phosphate buffer and 0.1 mol/L KCl.We obtained a calibration plot of the percentage inhibition versus the logarithm of parathion methyl concentration following an incubation time of 10 mix in parathion methyl solution. The lowest detectable amount of parathion methyl was 0.026 ppm.The amperometric biosensor based on acetylcholinesterase was disposable and low cost (about 1 yuan RMB). 展开更多
关键词 amperometric biosensor ACHE screen-printed electrodes organophosphorus pesticides
下载PDF
New Solution of Substrate Concentration in the Biosensor Response by Discrete Homotopy Analysis Method
17
作者 Seyyed Ali Madani Tonekaboni Ali Shahbazi Mastan Abad +1 位作者 Shahab Karimi Mitra Shabani 《World Journal of Engineering and Technology》 2013年第3期27-32,共6页
In this article, Discrete Homotopy Analysis Method (DHAM), as a new numerical method, is employed to investigate amperometric biosensor at mixed enzyme kinetics and diffusion limitation. Mathematical modeling of the p... In this article, Discrete Homotopy Analysis Method (DHAM), as a new numerical method, is employed to investigate amperometric biosensor at mixed enzyme kinetics and diffusion limitation. Mathematical modeling of the problem is developed utilizing non-Michaelis-Menten kinetics of the enzymatic reaction. Different results are obtained for different values of the dimensionless parameters described in the paper. The presented solution is then compared with the available actual and simulated results. 展开更多
关键词 DISCRETE HOMOTOPY Analysis Method amperometric biosensor MATHEMATICAL Modeling Non-Michaelis-Menten Kinetics
下载PDF
钯-四氧化三铁纳米复合物生物传感器的制备及检测有机磷农药的研究 被引量:2
18
作者 李灿鹏 杨龙 +2 位作者 庞芳 杨周 王光灿 《分析试验室》 CAS CSCD 北大核心 2013年第3期5-9,共5页
合成钯-四氧化三铁纳米粒子(Pd-Fe3O4NPs)并制备了乙酰胆碱酯酶(AChE)电流型生物传感器用于检测有机磷杀虫剂。Pd-Fe3O4NPs在电极与乙酰胆碱酯酶之间提供了一个具有较高电子传导性、较好生物相容性和较强催化活性的微环境,有效地提高了... 合成钯-四氧化三铁纳米粒子(Pd-Fe3O4NPs)并制备了乙酰胆碱酯酶(AChE)电流型生物传感器用于检测有机磷杀虫剂。Pd-Fe3O4NPs在电极与乙酰胆碱酯酶之间提供了一个具有较高电子传导性、较好生物相容性和较强催化活性的微环境,有效地提高了乙酰胆碱酯酶的生物活性和有机磷杀虫剂对乙酰胆碱酯酶的抑制作用。以对硫磷为有机磷杀虫剂模型分子,乙酰胆碱酶的抑制率与对硫磷浓度在5×10-12~1×10-9mol/L之间呈现良好的线性关系,检测限为2.5×10-12mol/L。 展开更多
关键词 Pd-Fe3O4 NPS 乙酰胆碱酯酶 电流型生物传感器 对硫磷
原文传递
高灵敏度双介体基胆固醇生物传感器的研制 被引量:2
19
作者 郭鼎力 曾义 +2 位作者 谢国雄 刘锡康 陈淑慧 《分析科学学报》 CAS CSCD 1999年第5期372-376,共5页
研制了一种高灵敏度、高准确性、微小取样(一滴血,V≥ 25 μL)并可快速测量(≤60 s)的实用型夹心式胆固醇生物传感器。它是由双介体、三酶生化氧还体系构成的电流型传感器。其中,第一介体含于敏感电极石墨电导层内,第二... 研制了一种高灵敏度、高准确性、微小取样(一滴血,V≥ 25 μL)并可快速测量(≤60 s)的实用型夹心式胆固醇生物传感器。它是由双介体、三酶生化氧还体系构成的电流型传感器。其中,第一介体含于敏感电极石墨电导层内,第二介体及三种酶、相伴试剂含于试剂纤维层中。本传感器可用来直接测量从正常人到异常人的血胆固醇含量(2~10m m ol/L),并将实现商品化。 展开更多
关键词 测定 血液 胆固醇传感器 生物传感器
下载PDF
一种新型无酶电流型葡萄糖生物传感器 被引量:1
20
作者 林丞 熊兆贤 +2 位作者 薛昊 陈淑娴 邱虹 《仪表技术与传感器》 CSCD 北大核心 2011年第2期4-5,8,共3页
采用电聚合过氧化聚吡咯(Ox-PPy)于铅笔芯电极(PGE),并将酞菁钴(CoPc)对其修饰,制作了一种新型无酶电流型葡萄糖生物传感器。实验结果表明:在优化条件下,传感器的灵敏度为6.42μA/(mmol/L),线性范围0~10 mmol/L,线性相关度R=0.992 9,... 采用电聚合过氧化聚吡咯(Ox-PPy)于铅笔芯电极(PGE),并将酞菁钴(CoPc)对其修饰,制作了一种新型无酶电流型葡萄糖生物传感器。实验结果表明:在优化条件下,传感器的灵敏度为6.42μA/(mmol/L),线性范围0~10 mmol/L,线性相关度R=0.992 9,响应时间小于10 s,最低检测限为70.1μmol/L.该传感器具有较高的灵敏度,较好的稳定性和一定的抗干扰能力。 展开更多
关键词 生物传感器 无酶 电流型 葡萄糖 酞菁钴 过氧化聚吡咯
下载PDF
上一页 1 2 下一页 到第
使用帮助 返回顶部