Alcoholism and cirrhosis,which are two of the most serious health problems worldwide,have a broad spectrum of clinical outcomes.Both diseases are influenced by genetic susceptibility and cultural traits that differ gl...Alcoholism and cirrhosis,which are two of the most serious health problems worldwide,have a broad spectrum of clinical outcomes.Both diseases are influenced by genetic susceptibility and cultural traits that differ globally but are specific for each population.In contrast to other regions around the world,Mexicans present the highest drinking score and a high mortality rate for alcoholic liver disease with an intermediate category level of per capita alcohol consumption.Mexico has a unique history of alcohol consumption that is linked to profound anthropological and social aspects.The Mexican population has an admixture genome inherited from different races,Caucasian,Amerindian and African,with a heterogeneous distribution within the country.Thus,genes related to alcohol addiction,such as dopamine receptor D2 in the brain,or liver alcoholmetabolizing enzymes,such as alcohol dehydrogenase classⅠpolypeptide B,cytochrome P450 2E1 and aldehyde dehydrogenase class 2,may vary from one individual to another.Furthermore,they may be inherited as risk or non-risk haplogroups that confer susceptibility or resistance either to alcohol addiction or abusive alcohol consumption and possibly liver disease.Thus,in this era of genomics,personalized medicine will benefit patients if it is directed according to individual or population-based data.Additional association studies will be required to establish novel strategies for the prevention,care and treatment of liver disease in Mexico and worldwide.展开更多
目的:研究丁苯酞(NBP)对酒精依赖大鼠海马谷氨酸(Glu)含量和NNMDA受体2B亚基(NR2B)表达的影响。方法:建立酒精成瘾大鼠模型,观察戒断症状,SYBR Green I荧光实时定量PCR技术检测海马区NR2BmRNA表达,高效液相色谱法检测海马组织中谷氨酸...目的:研究丁苯酞(NBP)对酒精依赖大鼠海马谷氨酸(Glu)含量和NNMDA受体2B亚基(NR2B)表达的影响。方法:建立酒精成瘾大鼠模型,观察戒断症状,SYBR Green I荧光实时定量PCR技术检测海马区NR2BmRNA表达,高效液相色谱法检测海马组织中谷氨酸含量。结果:模型组大鼠戒断评分比正常组明显上升(P<0.01),NBP中、高剂量组与模型组相比,戒断评分明显下降(P<0.05),差异均有显著性;模型组大鼠海马区谷氨酸含量较正常组显著降低(P<0.01),差异有显著性,而各用药组与模型组相比,海马区谷氨酸含量差异无显著性(P>0.05);实时定量PCR结果表明模型组大鼠海马区NR2BmRNA表达较正常组明显增加(P<0.05),而NBP中、高剂量组与模型组相比,海马区NR2BmRNA表达明显减少,差异有显著性(P<0.05)。结论:NBP能够减轻酒精依赖大鼠的戒断症状,可能与NBP抑制NR2BmRNA表达有关。展开更多
基金Supported by The National Council of Science and Technology,(Conacyt-Fondo Sectorial,Mexico),Grant No.Salud-2010-1-139085 awarded to Roman S
文摘Alcoholism and cirrhosis,which are two of the most serious health problems worldwide,have a broad spectrum of clinical outcomes.Both diseases are influenced by genetic susceptibility and cultural traits that differ globally but are specific for each population.In contrast to other regions around the world,Mexicans present the highest drinking score and a high mortality rate for alcoholic liver disease with an intermediate category level of per capita alcohol consumption.Mexico has a unique history of alcohol consumption that is linked to profound anthropological and social aspects.The Mexican population has an admixture genome inherited from different races,Caucasian,Amerindian and African,with a heterogeneous distribution within the country.Thus,genes related to alcohol addiction,such as dopamine receptor D2 in the brain,or liver alcoholmetabolizing enzymes,such as alcohol dehydrogenase classⅠpolypeptide B,cytochrome P450 2E1 and aldehyde dehydrogenase class 2,may vary from one individual to another.Furthermore,they may be inherited as risk or non-risk haplogroups that confer susceptibility or resistance either to alcohol addiction or abusive alcohol consumption and possibly liver disease.Thus,in this era of genomics,personalized medicine will benefit patients if it is directed according to individual or population-based data.Additional association studies will be required to establish novel strategies for the prevention,care and treatment of liver disease in Mexico and worldwide.
文摘目的:研究丁苯酞(NBP)对酒精依赖大鼠海马谷氨酸(Glu)含量和NNMDA受体2B亚基(NR2B)表达的影响。方法:建立酒精成瘾大鼠模型,观察戒断症状,SYBR Green I荧光实时定量PCR技术检测海马区NR2BmRNA表达,高效液相色谱法检测海马组织中谷氨酸含量。结果:模型组大鼠戒断评分比正常组明显上升(P<0.01),NBP中、高剂量组与模型组相比,戒断评分明显下降(P<0.05),差异均有显著性;模型组大鼠海马区谷氨酸含量较正常组显著降低(P<0.01),差异有显著性,而各用药组与模型组相比,海马区谷氨酸含量差异无显著性(P>0.05);实时定量PCR结果表明模型组大鼠海马区NR2BmRNA表达较正常组明显增加(P<0.05),而NBP中、高剂量组与模型组相比,海马区NR2BmRNA表达明显减少,差异有显著性(P<0.05)。结论:NBP能够减轻酒精依赖大鼠的戒断症状,可能与NBP抑制NR2BmRNA表达有关。
