目的通过测定哮喘大鼠气道重塑后表皮生长因子蛋白的表达,探讨平喘颗粒对哮喘大鼠气道重塑的作用机理。方法将90只Wistar大鼠分为6组,每组15只,A、B组予以生理盐水灌胃,C组予0.5 mg布地奈德雾化吸入(2次/d),D、E、F组给予中药低4.01 g/(...目的通过测定哮喘大鼠气道重塑后表皮生长因子蛋白的表达,探讨平喘颗粒对哮喘大鼠气道重塑的作用机理。方法将90只Wistar大鼠分为6组,每组15只,A、B组予以生理盐水灌胃,C组予0.5 mg布地奈德雾化吸入(2次/d),D、E、F组给予中药低4.01 g/(kg·d)、中8.01 g/(kg·d)、高16.02 g/(kg·d)剂量灌胃。各组均于末次激发24 h后处死,取大鼠左肺中下段,切成1 mm3左右,并将其用2.5%戊二醛固定,以备电镜用。大鼠右肺切取后,先用PBS漂洗,吸干后置于液氮罐中速冻,之后保存于-80℃冰箱,用于RT-PCR检测。结果平喘颗粒(低、中、高)组、布地奈德组和模型组的EGF m RNA蛋白表达均高于空白组,差异有统计学意义(P<0.05)。平喘颗粒(低、中、高)组和布地奈德组EGF m RNA蛋白的表达较模型组明显受到抑制,差异有统计学意义(P<0.05)。平喘颗粒(中、高)组EGF m RNA蛋白的表达较布地奈德组降低,差异有统计学意义(P<0.05)。平喘颗粒低剂量组EGF m RNA蛋白的表达较布地奈德组降低,但差异无统计学意义。结论平喘颗粒可以改善和控制气道重塑过程中气道高反应和气道阻塞的状态,并且可抑制EGF m RNA的表达,从而保护肺部组织细胞,减轻哮喘发作。展开更多
In order to investigate the effect of nuclear factor κB (NF κB) on airway remodeling in asthmatic rats, 18 Wistar rats were divided into three groups: asthmatic group; pyrrolidine dithiocarbamate (PDTC) group, in...In order to investigate the effect of nuclear factor κB (NF κB) on airway remodeling in asthmatic rats, 18 Wistar rats were divided into three groups: asthmatic group; pyrrolidine dithiocarbamate (PDTC) group, in which rats were injected intraperitoneally with NF κB specific inhibitor PDTC (100 mg/kg) before ovalbumin (OVA) challenge; control group. The NF κB activity and the expression of inhibitory protein κBα (I κBα) in airway were detected by electrophoretic mobility shift assay (EMSA), Western blot and immunohistochemistry respectively. The infiltration of inflammatory cells, the number of Goblet cells, the area of collagen and smooth muscle in airway were measured by means of image analysis system. The results showed that with the up regulation of airway NF κB activity in asthmatic group, the number of goblet cells (3.08±0.86/100 μm basement membrane (BM)), the area of collagen (24.71±4.24 μm 2/μm BM) and smooth muscle (13.81±2.11 μm 2/μm BM) in airway were significantly increased ( P <0.05) as compared with control group (0.14±0.05/100 μm BM, 14.31 ±3.16 μm 2/μm BM and 7.67±2.35 μm 2/μm BM respectively) and PDTC group (0.33±0.14/100 μm BM, 18.16±2.85 μm 2/μm BM and 8.95±2.16 μm 2/μm BM respectively). However, there was no significant difference between PDTC group and control group ( P >0.05). It was concluded that the activity of NF κB is increased in airway of asthmatic rats. Inhibition of NF κB activation can attenuate constructional changes in asthma airway, suggesting NF κB may contribute to asthmatic airway remodeling.展开更多
文摘目的通过测定哮喘大鼠气道重塑后表皮生长因子蛋白的表达,探讨平喘颗粒对哮喘大鼠气道重塑的作用机理。方法将90只Wistar大鼠分为6组,每组15只,A、B组予以生理盐水灌胃,C组予0.5 mg布地奈德雾化吸入(2次/d),D、E、F组给予中药低4.01 g/(kg·d)、中8.01 g/(kg·d)、高16.02 g/(kg·d)剂量灌胃。各组均于末次激发24 h后处死,取大鼠左肺中下段,切成1 mm3左右,并将其用2.5%戊二醛固定,以备电镜用。大鼠右肺切取后,先用PBS漂洗,吸干后置于液氮罐中速冻,之后保存于-80℃冰箱,用于RT-PCR检测。结果平喘颗粒(低、中、高)组、布地奈德组和模型组的EGF m RNA蛋白表达均高于空白组,差异有统计学意义(P<0.05)。平喘颗粒(低、中、高)组和布地奈德组EGF m RNA蛋白的表达较模型组明显受到抑制,差异有统计学意义(P<0.05)。平喘颗粒(中、高)组EGF m RNA蛋白的表达较布地奈德组降低,差异有统计学意义(P<0.05)。平喘颗粒低剂量组EGF m RNA蛋白的表达较布地奈德组降低,但差异无统计学意义。结论平喘颗粒可以改善和控制气道重塑过程中气道高反应和气道阻塞的状态,并且可抑制EGF m RNA的表达,从而保护肺部组织细胞,减轻哮喘发作。
文摘In order to investigate the effect of nuclear factor κB (NF κB) on airway remodeling in asthmatic rats, 18 Wistar rats were divided into three groups: asthmatic group; pyrrolidine dithiocarbamate (PDTC) group, in which rats were injected intraperitoneally with NF κB specific inhibitor PDTC (100 mg/kg) before ovalbumin (OVA) challenge; control group. The NF κB activity and the expression of inhibitory protein κBα (I κBα) in airway were detected by electrophoretic mobility shift assay (EMSA), Western blot and immunohistochemistry respectively. The infiltration of inflammatory cells, the number of Goblet cells, the area of collagen and smooth muscle in airway were measured by means of image analysis system. The results showed that with the up regulation of airway NF κB activity in asthmatic group, the number of goblet cells (3.08±0.86/100 μm basement membrane (BM)), the area of collagen (24.71±4.24 μm 2/μm BM) and smooth muscle (13.81±2.11 μm 2/μm BM) in airway were significantly increased ( P <0.05) as compared with control group (0.14±0.05/100 μm BM, 14.31 ±3.16 μm 2/μm BM and 7.67±2.35 μm 2/μm BM respectively) and PDTC group (0.33±0.14/100 μm BM, 18.16±2.85 μm 2/μm BM and 8.95±2.16 μm 2/μm BM respectively). However, there was no significant difference between PDTC group and control group ( P >0.05). It was concluded that the activity of NF κB is increased in airway of asthmatic rats. Inhibition of NF κB activation can attenuate constructional changes in asthma airway, suggesting NF κB may contribute to asthmatic airway remodeling.
