AIM: To describe and compare the differences in electroretinographic responses between two different age groups of adult Dark Agouti (DA) rats and to better understand the effect of age on retinal histology and functi...AIM: To describe and compare the differences in electroretinographic responses between two different age groups of adult Dark Agouti (DA) rats and to better understand the effect of age on retinal histology and function. METHODS: The electroretinographic responses of two different age groups of adult DA rats were compared. Animals were divided into younger adult DA rats 10-12wk (n =8) and older adult DA rats 17-19wk (n=8). Full field electroretinography (ERG) was recorded simultaneously from both eyes after dark adaption and light adaption and parameters including the positive scotopic threshold response (pSTR), negative scotopic threshold response (nSTR), scotopic a -wave, b -wave, photopic a -wave, b -wave and photopic negative response (PhNR) were compared between groups. RESULTS: The older adult rats displayed lower stimulation thresholds of the STRs (pSTR and nSTR) and higher amplitudes of pSTR, scotopic a -wave and b wave, photopic b -wave and PhNR amplitudes, with shorter implicit times. Photopic a-wave amplitudes were however higher in the younger adult rats. CONCLUSION: In summary, for the rod system, photoreceptor, bipolar cell and RGC activity was enhanced in the older adult rats. For the cone system, RGC and bipolar cell activity was enhanced, while photoreceptor activity was depressed in the older adult rats. Such age -related selective modification of retinal cell function needs to be considered when conducting ophthalmic research in adult rats.展开更多
旨在探讨Agouti基因调控山羊毛色机制,采用SYBR Green real-time PCR和蛋白免疫印迹方法分别检测了不同毛色山羊皮肤组织中Agouti基因mRNA及其所编码ASIP蛋白的差异表达。结果表明,Agouti基因mRNA在白色、黑色、棕色山羊皮肤组织中的表...旨在探讨Agouti基因调控山羊毛色机制,采用SYBR Green real-time PCR和蛋白免疫印迹方法分别检测了不同毛色山羊皮肤组织中Agouti基因mRNA及其所编码ASIP蛋白的差异表达。结果表明,Agouti基因mRNA在白色、黑色、棕色山羊皮肤组织中的表达量依次为白色>黑色>棕色;mRNA表达量在棕色和白色、棕色和黑色山羊皮肤之间均存在显著差异(P<0.05),在白色和黑色山羊皮肤之间差异不显著(P>0.05)。ASIP在白色、黑色、棕色山羊皮肤组织中的表达量:白色>棕色>黑色;ASIP表达量在白色和黑色山羊皮肤组织之间存在显著差异(P<0.05),但在白色和棕色、棕色和黑色山羊皮肤组织之间差异均不显著(P>0.05)。研究结果提示,Agouti基因在不同毛色山羊皮肤组织中可能存在不同调控机制。展开更多
基金Supported by the Seed Grant for Basic Research,Research Services,The University of Hong Kong
文摘AIM: To describe and compare the differences in electroretinographic responses between two different age groups of adult Dark Agouti (DA) rats and to better understand the effect of age on retinal histology and function. METHODS: The electroretinographic responses of two different age groups of adult DA rats were compared. Animals were divided into younger adult DA rats 10-12wk (n =8) and older adult DA rats 17-19wk (n=8). Full field electroretinography (ERG) was recorded simultaneously from both eyes after dark adaption and light adaption and parameters including the positive scotopic threshold response (pSTR), negative scotopic threshold response (nSTR), scotopic a -wave, b -wave, photopic a -wave, b -wave and photopic negative response (PhNR) were compared between groups. RESULTS: The older adult rats displayed lower stimulation thresholds of the STRs (pSTR and nSTR) and higher amplitudes of pSTR, scotopic a -wave and b wave, photopic b -wave and PhNR amplitudes, with shorter implicit times. Photopic a-wave amplitudes were however higher in the younger adult rats. CONCLUSION: In summary, for the rod system, photoreceptor, bipolar cell and RGC activity was enhanced in the older adult rats. For the cone system, RGC and bipolar cell activity was enhanced, while photoreceptor activity was depressed in the older adult rats. Such age -related selective modification of retinal cell function needs to be considered when conducting ophthalmic research in adult rats.
文摘旨在探讨Agouti基因调控山羊毛色机制,采用SYBR Green real-time PCR和蛋白免疫印迹方法分别检测了不同毛色山羊皮肤组织中Agouti基因mRNA及其所编码ASIP蛋白的差异表达。结果表明,Agouti基因mRNA在白色、黑色、棕色山羊皮肤组织中的表达量依次为白色>黑色>棕色;mRNA表达量在棕色和白色、棕色和黑色山羊皮肤之间均存在显著差异(P<0.05),在白色和黑色山羊皮肤之间差异不显著(P>0.05)。ASIP在白色、黑色、棕色山羊皮肤组织中的表达量:白色>棕色>黑色;ASIP表达量在白色和黑色山羊皮肤组织之间存在显著差异(P<0.05),但在白色和棕色、棕色和黑色山羊皮肤组织之间差异均不显著(P>0.05)。研究结果提示,Agouti基因在不同毛色山羊皮肤组织中可能存在不同调控机制。
