Oral lichen planus （OLP） is a chronic inflammatory disease that is frequently detected in oral tissues. The aim of our study was to identify the prevalence of the detection of periodontopathogenic microorganisms （A...Oral lichen planus （OLP） is a chronic inflammatory disease that is frequently detected in oral tissues. The aim of our study was to identify the prevalence of the detection of periodontopathogenic microorganisms （Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola in OLP patients and to compare with this prevalence of periodontopathogenic microorganisms in healthy non-OLP patients. Our study included 27 （18 chronic periodontitis （OLPP） and 9 gingivitis （OLPG）） patients diagnosed with OLP along with 26 （13 chronic periodontitis （HP） and 13 gingivitis （HG）） healthy non-OLP patients. The multiplex polymerase chain reaction （PCR）with subsequent reverse hybridization method （micro-IDent） was used for identifying periodontopathogenic microorganisms present in subgingival plaque samples. The percentages of detection for A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola in subgingival plaque samples taken from OLP patients （OLPG and OLPP） were 18.5%, 85.1%, 81.4%, 88.8% and 74%, respectively. Meanwhile, in the non-OLP patients （HG and HP）, these values were 7.6%, 50%, 46.1%, 73% and 57.7%, respectively. Thus, comparing the non-OLP groups with the OLP groups, the periodontopathogens＇ percentages of detection in the OLP groups were higher than those in the non-OLP groups. According to our study results, OLP patients have higher levels of infection with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola than non-OLP patients. We argue that the high percentages in patients with OLP may help identify the importance of periodontopathoRenic microorganisms in the progress of periodontal diseases of OLP.展开更多
Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects ind...Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) production in A. actinomycetemcomitans-immunized mice. Materials and Methods: BALB/c mice were sham-immunized (group I) or immunized with heat-killed A. actinomycetemcomitans (group II-VII). Colchicine was injected intraperitoneally before (group III), on the same day of (group IV), or after (group V) the primary immunization and on the same day of (group VI) or after (group VII) the secondary immunization. In vitro, spleen cells from either sham- or heat-killed A. actinomycetemcomitan-immunized animals were cultured and stimulated with heat-killed A. actinomycetemcomitans in the presence or absence of colchicine with or without addition of L-arginine, Db-cAMP, forskolin or interferon-γ (IFN-γ). The levels of splenic iNOS activity and both serum and culture supernatant NO levels were assessed. Results: The results showed that colchicine did inhibit both splenic iNOS activity and serum NO levels only when the drug was injected at the same time as the immunization (group IV and VI). Splenic iNOS activity and NO levels on antigen-stimulated spleen cell cultures were also suppressed by colchicine, even in the presence of L-arginine, Db-AMP or forskolin. IFN-γ only partially restored iNOS activity and NO levels in the antigen and colchicine-treated spleen cell cultures. Conclusion: This study suggests, therefore, that colchicine may suppress the iNOS activity and NO production in A. actinomycetemcomitans-immunized mice in vivo and in vitro.展开更多
目的探讨磷脂酰胆碱在伴放线放线杆菌(Aggregatibacter actinomycetemcomitans,Aa)对人脐静脉血管内皮细胞粘附和侵袭过程中的作用。方法采集7例侵袭性牙周炎患者牙周袋内的非附着性菌斑,接种于Aa选择性培养板上,根据菌落的形态、触酶...目的探讨磷脂酰胆碱在伴放线放线杆菌(Aggregatibacter actinomycetemcomitans,Aa)对人脐静脉血管内皮细胞粘附和侵袭过程中的作用。方法采集7例侵袭性牙周炎患者牙周袋内的非附着性菌斑,接种于Aa选择性培养板上,根据菌落的形态、触酶实验、革兰氏染色实验及16S r DNA鉴定及细菌表面蛋白鉴定,筛选出磷脂酰胆碱阳性表达的Aa菌株,并通过粘附侵袭实验,研究磷脂酰胆碱在Aa致病中的作用。结果侵袭性牙周炎患者的龈下菌斑通过Aa的选择性培养及鉴定,筛选出了1株磷脂酰胆碱阳性的Aa菌株,在粘附和侵袭实验中,发现磷脂酰胆碱被其特异性单克隆抗体小鼠抗磷脂酰胆碱单克隆抗体TEPC-15阻断后,磷脂酰胆碱阳性的Aa对人脐静脉血管内皮细胞的粘附率和侵袭率分别为对照组的(31.87±4.22)%和(24.63±3.55)%,粘附率(t=27.981,P=0.001)和侵袭率(t=36.786,P=0.001)与对照组相比差异均有统计学意义。结论磷脂酰胆碱有助于Aa对人脐静脉血管内皮细胞的粘附和入侵。展开更多
基金project support from the Turkey Scientific and Technological Research Council (project no. 106S340)Selcuk University Coordination of Scientific Research (project no. 06202034)supported by Open Fund of State Key Laboratory of Oral Diseases, Sichuan University
文摘Oral lichen planus （OLP） is a chronic inflammatory disease that is frequently detected in oral tissues. The aim of our study was to identify the prevalence of the detection of periodontopathogenic microorganisms （Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola in OLP patients and to compare with this prevalence of periodontopathogenic microorganisms in healthy non-OLP patients. Our study included 27 （18 chronic periodontitis （OLPP） and 9 gingivitis （OLPG）） patients diagnosed with OLP along with 26 （13 chronic periodontitis （HP） and 13 gingivitis （HG）） healthy non-OLP patients. The multiplex polymerase chain reaction （PCR）with subsequent reverse hybridization method （micro-IDent） was used for identifying periodontopathogenic microorganisms present in subgingival plaque samples. The percentages of detection for A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola in subgingival plaque samples taken from OLP patients （OLPG and OLPP） were 18.5%, 85.1%, 81.4%, 88.8% and 74%, respectively. Meanwhile, in the non-OLP patients （HG and HP）, these values were 7.6%, 50%, 46.1%, 73% and 57.7%, respectively. Thus, comparing the non-OLP groups with the OLP groups, the periodontopathogens＇ percentages of detection in the OLP groups were higher than those in the non-OLP groups. According to our study results, OLP patients have higher levels of infection with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola than non-OLP patients. We argue that the high percentages in patients with OLP may help identify the importance of periodontopathoRenic microorganisms in the progress of periodontal diseases of OLP.
文摘Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) production in A. actinomycetemcomitans-immunized mice. Materials and Methods: BALB/c mice were sham-immunized (group I) or immunized with heat-killed A. actinomycetemcomitans (group II-VII). Colchicine was injected intraperitoneally before (group III), on the same day of (group IV), or after (group V) the primary immunization and on the same day of (group VI) or after (group VII) the secondary immunization. In vitro, spleen cells from either sham- or heat-killed A. actinomycetemcomitan-immunized animals were cultured and stimulated with heat-killed A. actinomycetemcomitans in the presence or absence of colchicine with or without addition of L-arginine, Db-cAMP, forskolin or interferon-γ (IFN-γ). The levels of splenic iNOS activity and both serum and culture supernatant NO levels were assessed. Results: The results showed that colchicine did inhibit both splenic iNOS activity and serum NO levels only when the drug was injected at the same time as the immunization (group IV and VI). Splenic iNOS activity and NO levels on antigen-stimulated spleen cell cultures were also suppressed by colchicine, even in the presence of L-arginine, Db-AMP or forskolin. IFN-γ only partially restored iNOS activity and NO levels in the antigen and colchicine-treated spleen cell cultures. Conclusion: This study suggests, therefore, that colchicine may suppress the iNOS activity and NO production in A. actinomycetemcomitans-immunized mice in vivo and in vitro.
文摘目的探讨磷脂酰胆碱在伴放线放线杆菌(Aggregatibacter actinomycetemcomitans,Aa)对人脐静脉血管内皮细胞粘附和侵袭过程中的作用。方法采集7例侵袭性牙周炎患者牙周袋内的非附着性菌斑,接种于Aa选择性培养板上,根据菌落的形态、触酶实验、革兰氏染色实验及16S r DNA鉴定及细菌表面蛋白鉴定,筛选出磷脂酰胆碱阳性表达的Aa菌株,并通过粘附侵袭实验,研究磷脂酰胆碱在Aa致病中的作用。结果侵袭性牙周炎患者的龈下菌斑通过Aa的选择性培养及鉴定,筛选出了1株磷脂酰胆碱阳性的Aa菌株,在粘附和侵袭实验中,发现磷脂酰胆碱被其特异性单克隆抗体小鼠抗磷脂酰胆碱单克隆抗体TEPC-15阻断后,磷脂酰胆碱阳性的Aa对人脐静脉血管内皮细胞的粘附率和侵袭率分别为对照组的(31.87±4.22)%和(24.63±3.55)%,粘附率(t=27.981,P=0.001)和侵袭率(t=36.786,P=0.001)与对照组相比差异均有统计学意义。结论磷脂酰胆碱有助于Aa对人脐静脉血管内皮细胞的粘附和入侵。
