This study investigated the effects of SIRT1 gene knock-out on osteoarthritis in mice, and the possible roles of SREBP2 protein and the PI3K/AKT signaling pathway in the effects. Mice were randomly divided into a norm...This study investigated the effects of SIRT1 gene knock-out on osteoarthritis in mice, and the possible roles of SREBP2 protein and the PI3K/AKT signaling pathway in the effects. Mice were randomly divided into a normal group and a SIRT1 gene knock-out group（6 mice in each group）. In these groups, one side of the knee anterior cruciate ligament was traversed, and the ipsilateral medial meniscus was cut to establish an osteoarthritis model of knee joint. The countralateral synovial bursa was cut out, serving as controls. The knee joint specimens were then divided into four groups： SIRT1^（＋/＋） control group（group A, n=6）; SIRT1^（＋/＋） osteoarthritis group（group B, n=6）; SIRT1^（–/–） control group（group C, n=6）; SIRT1^（–/–） osteoarthritis group（group D, n=6）. HE staining, Masson staining, Safranin O-Fast Green staining and Van Gieson staining were used to observe the morphological changes in the articular cartilage of the knee. Immunohistochemical staining was employed to detect the expression of SIRT1, SREBP2, VEGF, AKT, HMGCR and type Ⅱ collagen proteins. SA-β-gal staining was utilized to evaluate chondrocyte aging. The results showed clear knee joint cartilage destruction and degeneration in the SIRT1^（–/–） osteoarthritis group. The tidal line was twisted and displaced anteriorly. Type Ⅱ collagen was destroyed and distributed unevenly. Compared with the SIRT1^（＋/＋） osteoarthritis group and SIRT1^（–/–） control group, SIRT1 protein expression was not obviously changed in the SIRT1^（–/–） osteoarthritis group（P〉0.05）, while the expression levels of the SREBP2, VEGF and HMGCR proteins were significantly increased（P〈0.05） and the levels of AKT and type Ⅱ collagen proteins were significantly decreased（P〈0.05）. SIRT1 gene knock-out may aggravate cartilage degeneration in osteoarthritis by activating the SREBP2 protein-mediated PI3K/AKT signalling pathway, suggesting that SIRT1 gene may play a protective role again展开更多
Mr.Chairman,Ladies and Gentlemen,Dear Friends,It is a great honor for me to share thoughts and ideas with all participants on Working Together to Address Terrorist Threats and Build a New Middle East of Peace,Toleranc...Mr.Chairman,Ladies and Gentlemen,Dear Friends,It is a great honor for me to share thoughts and ideas with all participants on Working Together to Address Terrorist Threats and Build a New Middle East of Peace,Tolerance and Self-Advancement.The situation in the Middle East region is serious.Differences among nations,religious sects and ethnic groups aggravate one another.Wars and conflicts,terrorist threats。展开更多
2008425 Difference in gene expression profile of plasma coagulation factors in patients with pulmonary embolism.CHENG Kebin(程克斌), et al. Dept Respir Med, Shanghai Pulm Hosp, Tongji Univ, Shanghai 200433. Shanghai M...2008425 Difference in gene expression profile of plasma coagulation factors in patients with pulmonary embolism.CHENG Kebin(程克斌), et al. Dept Respir Med, Shanghai Pulm Hosp, Tongji Univ, Shanghai 200433. Shanghai Med J 2008:31(6):381-384. Objective To identify the association between the mRNA expression of blood coagulation factors and pulmonary embolism (PE) by comparing the gene expression profiles of patients with PE and normal controls using gene microarray.展开更多
In early June,two lead factories of Henan Jinli,and Yuguang Gold&Lead announced they have obtained silver concentrate Processing Handbook.Market analysts considered that decontrol on contract processing of silver ...In early June,two lead factories of Henan Jinli,and Yuguang Gold&Lead announced they have obtained silver concentrate Processing Handbook.Market analysts considered that decontrol on contract processing of silver ore will aggravate the pressure on lead ingot supply in China.展开更多
基金supported by grants from the National Natural Science Foundation of China(No.81272032)the Research and Development Projects of Shenzhen of China(Nos.JCYJ20150403091443275)
文摘This study investigated the effects of SIRT1 gene knock-out on osteoarthritis in mice, and the possible roles of SREBP2 protein and the PI3K/AKT signaling pathway in the effects. Mice were randomly divided into a normal group and a SIRT1 gene knock-out group（6 mice in each group）. In these groups, one side of the knee anterior cruciate ligament was traversed, and the ipsilateral medial meniscus was cut to establish an osteoarthritis model of knee joint. The countralateral synovial bursa was cut out, serving as controls. The knee joint specimens were then divided into four groups： SIRT1^（＋/＋） control group（group A, n=6）; SIRT1^（＋/＋） osteoarthritis group（group B, n=6）; SIRT1^（–/–） control group（group C, n=6）; SIRT1^（–/–） osteoarthritis group（group D, n=6）. HE staining, Masson staining, Safranin O-Fast Green staining and Van Gieson staining were used to observe the morphological changes in the articular cartilage of the knee. Immunohistochemical staining was employed to detect the expression of SIRT1, SREBP2, VEGF, AKT, HMGCR and type Ⅱ collagen proteins. SA-β-gal staining was utilized to evaluate chondrocyte aging. The results showed clear knee joint cartilage destruction and degeneration in the SIRT1^（–/–） osteoarthritis group. The tidal line was twisted and displaced anteriorly. Type Ⅱ collagen was destroyed and distributed unevenly. Compared with the SIRT1^（＋/＋） osteoarthritis group and SIRT1^（–/–） control group, SIRT1 protein expression was not obviously changed in the SIRT1^（–/–） osteoarthritis group（P〉0.05）, while the expression levels of the SREBP2, VEGF and HMGCR proteins were significantly increased（P〈0.05） and the levels of AKT and type Ⅱ collagen proteins were significantly decreased（P〈0.05）. SIRT1 gene knock-out may aggravate cartilage degeneration in osteoarthritis by activating the SREBP2 protein-mediated PI3K/AKT signalling pathway, suggesting that SIRT1 gene may play a protective role again
文摘Mr.Chairman,Ladies and Gentlemen,Dear Friends,It is a great honor for me to share thoughts and ideas with all participants on Working Together to Address Terrorist Threats and Build a New Middle East of Peace,Tolerance and Self-Advancement.The situation in the Middle East region is serious.Differences among nations,religious sects and ethnic groups aggravate one another.Wars and conflicts,terrorist threats。
文摘2008425 Difference in gene expression profile of plasma coagulation factors in patients with pulmonary embolism.CHENG Kebin(程克斌), et al. Dept Respir Med, Shanghai Pulm Hosp, Tongji Univ, Shanghai 200433. Shanghai Med J 2008:31(6):381-384. Objective To identify the association between the mRNA expression of blood coagulation factors and pulmonary embolism (PE) by comparing the gene expression profiles of patients with PE and normal controls using gene microarray.
文摘In early June,two lead factories of Henan Jinli,and Yuguang Gold&Lead announced they have obtained silver concentrate Processing Handbook.Market analysts considered that decontrol on contract processing of silver ore will aggravate the pressure on lead ingot supply in China.
