Human serum albumin （HSA） is an abundant protein in plasma that can bind and transport many small molecules, and the corresponding affinity-controlled drug delivery shows great advantage in the biological system. Pe...Human serum albumin （HSA） is an abundant protein in plasma that can bind and transport many small molecules, and the corresponding affinity-controlled drug delivery shows great advantage in the biological system. Peptide SA06 is a reported ligand comprising 20 amino acids, and is known to non-covalently bind with HSA to extend the lifetime and improve the pharmacokinetic performance. The structural information of the HSA-peptide complex is keen for obtainingmolecular insight of the binding mechanism. We studied the secondary structural change and structure-affinity relations of Peptide SA06 with HSA by using circular dichroism （CD） spectroscopy in solution. Noticeable allosteric effect can be identified by compositional increase of a-helix structures when the peptide was co-incubated with HSA. Furthermore, the equilibrium dissociation constant of Peptide SA06 with HSA can be determined by CD-baged method. This work provides structural evidence on the allosteric interaction between peptide ligand and HSA, and sheds light on optimization of therapeutic properties in the affinity-controlled delivery systems.展开更多
基金Acknowledgement This work was supported by the National Natural Science Foundation of China （Nos. 21273051, 21673055）. The financial supports fi＇om the CAS key Laboratory of Standardization and Measurement for Nanotechnology, and the CAS key Laboratory for Biological Effects of Nanomaterials and Nanosafety are also gratefully acknowledged.
文摘Human serum albumin （HSA） is an abundant protein in plasma that can bind and transport many small molecules, and the corresponding affinity-controlled drug delivery shows great advantage in the biological system. Peptide SA06 is a reported ligand comprising 20 amino acids, and is known to non-covalently bind with HSA to extend the lifetime and improve the pharmacokinetic performance. The structural information of the HSA-peptide complex is keen for obtainingmolecular insight of the binding mechanism. We studied the secondary structural change and structure-affinity relations of Peptide SA06 with HSA by using circular dichroism （CD） spectroscopy in solution. Noticeable allosteric effect can be identified by compositional increase of a-helix structures when the peptide was co-incubated with HSA. Furthermore, the equilibrium dissociation constant of Peptide SA06 with HSA can be determined by CD-baged method. This work provides structural evidence on the allosteric interaction between peptide ligand and HSA, and sheds light on optimization of therapeutic properties in the affinity-controlled delivery systems.
