Sensitive smell discrimination is based on structural plasticity of the olfactory bulb,which depends on migration and integration of newborn neurons from the subventricular zone.In this study,we examined the relations...Sensitive smell discrimination is based on structural plasticity of the olfactory bulb,which depends on migration and integration of newborn neurons from the subventricular zone.In this study,we examined the relationship between neural stem cell status in the subventricular zone and olfactory function in rats with diabetes mellitus.Streptozotocin was injected through the femoral vein to induce type 1 diabetes mellitus in Sprague-Dawley rats.Two months after injection,olfactory sensitivity was decreased in diabetic rats.Meanwhile,the number of Brd U-positive and Brd U＋/DCX＋double-labeled cells was lower in the subventricular zone of diabetic rats compared with agematched normal rats.Western blot results revealed downregulated expression of insulin receptorβ,phosphorylated glycogen synthase kinase 3β,and β-catenin in the subventricular zone of diabetic rats.Altogether,these results indicate that diabetes mellitus causes insulin deficiency,which negatively regulates glycogen synthase kinase 3β and enhances β-catenin degradation,with these changes inhibiting neural stem cell proliferation.Further,these signaling pathways affect proliferation and differentiation of neural stem cells in the subventricular zone.Dysfunction of subventricular zone neural stem cells causes a decline in olfactory bulb structural plasticity and impairs olfactory sensitivity in diabetic rats.展开更多
目的观察异丙酚对体外培养的大鼠成年神经干细胞是否具有生长调控作用及可能的分子机制。方法大鼠海马成年神经干细胞接种贴壁后，模拟临床应用浓度加入异丙酚至终浓度10、25、50、100mg／L，溶剂（脂肪乳）为对照，培养24h。用DAPI染...目的观察异丙酚对体外培养的大鼠成年神经干细胞是否具有生长调控作用及可能的分子机制。方法大鼠海马成年神经干细胞接种贴壁后，模拟临床应用浓度加入异丙酚至终浓度10、25、50、100mg／L，溶剂（脂肪乳）为对照，培养24h。用DAPI染核，检测细胞数目变化。用免疫印记法检测细胞环磷腺苷反应元件结合蛋白（cAMP response element binding protein，CREB）磷酸化水平变化。结果异丙酚（〉10mg／L）处理组比对照组细胞数显著升高，且呈剂量依赖性。异丙酚处理前后，细胞均表达CREB和磷酸化CREB，但处理组比对照组的CREB磷酸化水平显著升高。结论异丙酚可能是通过上调细胞中CREB的磷酸化水平从而促进大鼠海马成年神经干细胞的增殖能力。展开更多
基金partly supported by the National Natural Science Foundation of China,No.81370448,81570725
文摘Sensitive smell discrimination is based on structural plasticity of the olfactory bulb,which depends on migration and integration of newborn neurons from the subventricular zone.In this study,we examined the relationship between neural stem cell status in the subventricular zone and olfactory function in rats with diabetes mellitus.Streptozotocin was injected through the femoral vein to induce type 1 diabetes mellitus in Sprague-Dawley rats.Two months after injection,olfactory sensitivity was decreased in diabetic rats.Meanwhile,the number of Brd U-positive and Brd U＋/DCX＋double-labeled cells was lower in the subventricular zone of diabetic rats compared with agematched normal rats.Western blot results revealed downregulated expression of insulin receptorβ,phosphorylated glycogen synthase kinase 3β,and β-catenin in the subventricular zone of diabetic rats.Altogether,these results indicate that diabetes mellitus causes insulin deficiency,which negatively regulates glycogen synthase kinase 3β and enhances β-catenin degradation,with these changes inhibiting neural stem cell proliferation.Further,these signaling pathways affect proliferation and differentiation of neural stem cells in the subventricular zone.Dysfunction of subventricular zone neural stem cells causes a decline in olfactory bulb structural plasticity and impairs olfactory sensitivity in diabetic rats.
文摘目的观察异丙酚对体外培养的大鼠成年神经干细胞是否具有生长调控作用及可能的分子机制。方法大鼠海马成年神经干细胞接种贴壁后，模拟临床应用浓度加入异丙酚至终浓度10、25、50、100mg／L，溶剂（脂肪乳）为对照，培养24h。用DAPI染核，检测细胞数目变化。用免疫印记法检测细胞环磷腺苷反应元件结合蛋白（cAMP response element binding protein，CREB）磷酸化水平变化。结果异丙酚（〉10mg／L）处理组比对照组细胞数显著升高，且呈剂量依赖性。异丙酚处理前后，细胞均表达CREB和磷酸化CREB，但处理组比对照组的CREB磷酸化水平显著升高。结论异丙酚可能是通过上调细胞中CREB的磷酸化水平从而促进大鼠海马成年神经干细胞的增殖能力。
