Physiological responses on the bases of activities of antioxidant enzymes: peroxidase, catalase, superoxide dismutase and glutathione reductase as well as estimation of total protein, lipid peroxidation and thiols ...Physiological responses on the bases of activities of antioxidant enzymes: peroxidase, catalase, superoxide dismutase and glutathione reductase as well as estimation of total protein, lipid peroxidation and thiols in the form of protein, non\|protein, glutathione and phytochelatin measured in growing seedlings of paddy, Oryza sativa L., from day 2 to 8 were compared following treatment of seeds for 5h with oxidative agents, paraquat 5 × 10 -5 , 10 -4 , 10 -3 mol/L, H 2O 2 10 -3 , 5×10 -3 , 10 -2 mol/L, and CdCl 2 10 -5 , 10 -4 , 5×10 -3 mol/L. A significant induction of all antioxidant enzymes along with an increase in the levels of protein, lipid peroxidation and glutathione was noted in response to oxidative stress, CdCl 2 induced significant peroxidase and catalase activities but not superoxide dismutase. In a marked contrast from oxidative stress, CdCl 2 decreased glutathione reductase activity as well as glutathione levels but increased phytochelatin level. The different physiological responses thus underlined the crucial involvement of glutathione and phytochelatin in the oxidative and heavy metal\|linduced adaptive responses respectively.展开更多
Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods:...Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods: We selected 12 beagles and divided them into two groups. One group was treated with dangshen decoction two hours before the flight, and the other group was untreated. Their routine blood indexes and clinical biochemical parameters were tested and analyzed before transport, after unloading and after adaptation for 1, 2, 3, 4, 5, and 6 days after administering dangshen.Results: We found that flight transportation stress adversely influenced many of the beagles' routine blood indexes. These recovered during adaptation, with dangshen administration assisting recovery of most indexes. Flight transport stress also adversely influenced biochemical indexes in the beagles. Again these recovered during adaptation, and dangshen aided in the recovery.Conclusion: Thus, we found that flight transport adversely affected the beagles' blood indexes, and dangshen reversed the damage from transport stress.展开更多
Chronic obstructive pulmonary disease(COPD)is a multifaceted syndrome characterized by a dysregulated inflammatory cascade within the respiratory system,primarily triggered by exposure to harmful particles and gases,n...Chronic obstructive pulmonary disease(COPD)is a multifaceted syndrome characterized by a dysregulated inflammatory cascade within the respiratory system,primarily triggered by exposure to harmful particles and gases,notably from cigarette smoke.This inflammatory response is orchestrated by innate immune cells like macrophages and epithelial cells,which recognize danger signals released from damaged cells.Prolonged inflammation prompts an adaptive immune reaction mediated by dendritic cells,culminating in the formation of lymphoid follicles and involving a complex interplay of T and B cells,as well as cytotoxic activity.Additionally,both viral and bacterial infections exacerbate COPD by further igniting inflammatory pathways,perpetuating the chronic inflammatory state.This comprehensive review encapsulates the intricate interplay between innate and adaptive immunity in COPD,with a particular focus on the role of cigarette smoke in its pathogenesis and potential therapeutic targets.展开更多
The molecular basis for adaptations to extreme environments can now be understood by interrogating the everincreasing number of sequenced genomes.Mammals such as cetaceans,bats,and highland species can protect themsel...The molecular basis for adaptations to extreme environments can now be understood by interrogating the everincreasing number of sequenced genomes.Mammals such as cetaceans,bats,and highland species can protect themselves from oxidative stress,a disruption in the balance of reactive oxygen species,which results in oxidative injury and cell damage.Here,we consider the evolution of the glutathione peroxidase(GPX)family of antioxidant enzymes by interrogating publicly available genome data from 70 mammalian species from all major clades.We identified 8 GPX subclasses ubiquitous to all mammalian groups.Mammalian GPX gene families resolved into the GPX4/7/8 and GPX1/2/3/5/6 groups and are characterized by several instances of gene duplication and loss,indicating a dynamic process of gene birth and death in mammals.Seven of the eight GPX subfamilies(all but GPX7)were under positive selection,with the residues under selection located at or close to active sites or at the dimer interface.We also reveal evidence of a correlation between ecological niches(e.g.high oxidative stress)and the divergent selection and gene copy number of GPX subclasses.Notably,a convergent expansion of GPX1 was observed in several independent lineages of mammals under oxidative stress and may be important for avoiding oxidative damage.Collectively,this study suggests that the GPX gene family has shaped the adaption of mammals to stressful environments.展开更多
The adaptive mesh mesh technique is developed and applied in three dimensional numerical simulation of welding process on the base of the commercial software. Special user subroutine is worked out to accom- plish t...The adaptive mesh mesh technique is developed and applied in three dimensional numerical simulation of welding process on the base of the commercial software. Special user subroutine is worked out to accom- plish this function.This technique can make the dense mesh moving simultaneously with the heat source while the other area of the structure with much coarser mesh, greatly reducing the number of nodes and elements in the analysis.Temperature field,displacement and stress distributions during welding pro- cess me analyzed by FEM method with adaptive mesh and the analysis is also conducted with normal FEM method. The temperature field,displacement and stress distributions obtained with both methods are shown in contrast. The results show that the temperature fields and the displacement distributions of simulation on adaptive mesh correspond wery well with that of without adaptive mesh. Though the stress distributions have some difference,but the trends of the stress distribution are corresponding.The com- parison of the computing time of the two meshes indicates that the adaptive that the adaptive mesh can greatly reduce the calculation time when used for welding process.展开更多
This paper presents the adaptive mesh finite element estimation method for analyzing 2D linear elastic fracture problems. The mesh is generated by the advancing front method and the norm stress error is taken as a pos...This paper presents the adaptive mesh finite element estimation method for analyzing 2D linear elastic fracture problems. The mesh is generated by the advancing front method and the norm stress error is taken as a posteriori error estimator for the h-type adaptive refinement. The stress intensity factors are estimated by a displacement extrapolation technique. The near crack tip displacements used are obtained from specific nodes of natural six-noded quarter-point elements which are generated around the crack tip defined by the user. The crack growth and its direction are determined by the calculated stress intensity factors. The maximum circumference theory is used for the latter. In evaluating the accuracy of the estimated stress intensity factors, four cases are tested consisting of compact tension specimen, three-point bending specimen, central cracked plate and double edge notched plate. These were carried out and compared to the results from other studies. The crack trajectories of these specimen tests are also illustrated.展开更多
Environmental change attracts particular attention by biologists concerned with the performance of biological systems under stress. To investigate these, dose–response relationships should be clarified. It was previo...Environmental change attracts particular attention by biologists concerned with the performance of biological systems under stress. To investigate these, dose–response relationships should be clarified. It was previously assumed that the fundamental nature of biological dose–responses follows a linear model, either with no threshold or with a threshold below which no effects are expected to occur in biological endpoints. However, substantial literature, including widespread documentation in plants, has revealed that hormesis commonly occurs. Hormesis is highly generalized and can be utilized as a quantitative measure of biological plasticity. Conditioning induced by adaptive responses also occurs in the framework of hormesis and is of particular importance to environmental change biology with regards to evolutionary adaptations.This paper presents additional evidence for hormetic dose responses induced by temperature in plants. The current understanding on hormesis provides a perspective for next generation environmental change research. Hormesis should have a central role in environmental change biology of vegetation.展开更多
文摘Physiological responses on the bases of activities of antioxidant enzymes: peroxidase, catalase, superoxide dismutase and glutathione reductase as well as estimation of total protein, lipid peroxidation and thiols in the form of protein, non\|protein, glutathione and phytochelatin measured in growing seedlings of paddy, Oryza sativa L., from day 2 to 8 were compared following treatment of seeds for 5h with oxidative agents, paraquat 5 × 10 -5 , 10 -4 , 10 -3 mol/L, H 2O 2 10 -3 , 5×10 -3 , 10 -2 mol/L, and CdCl 2 10 -5 , 10 -4 , 5×10 -3 mol/L. A significant induction of all antioxidant enzymes along with an increase in the levels of protein, lipid peroxidation and glutathione was noted in response to oxidative stress, CdCl 2 induced significant peroxidase and catalase activities but not superoxide dismutase. In a marked contrast from oxidative stress, CdCl 2 decreased glutathione reductase activity as well as glutathione levels but increased phytochelatin level. The different physiological responses thus underlined the crucial involvement of glutathione and phytochelatin in the oxidative and heavy metal\|linduced adaptive responses respectively.
基金Guangdong Science and Technology Department,Grant/Award Number:2015A030302073
文摘Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods: We selected 12 beagles and divided them into two groups. One group was treated with dangshen decoction two hours before the flight, and the other group was untreated. Their routine blood indexes and clinical biochemical parameters were tested and analyzed before transport, after unloading and after adaptation for 1, 2, 3, 4, 5, and 6 days after administering dangshen.Results: We found that flight transportation stress adversely influenced many of the beagles' routine blood indexes. These recovered during adaptation, with dangshen administration assisting recovery of most indexes. Flight transport stress also adversely influenced biochemical indexes in the beagles. Again these recovered during adaptation, and dangshen aided in the recovery.Conclusion: Thus, we found that flight transport adversely affected the beagles' blood indexes, and dangshen reversed the damage from transport stress.
文摘Chronic obstructive pulmonary disease(COPD)is a multifaceted syndrome characterized by a dysregulated inflammatory cascade within the respiratory system,primarily triggered by exposure to harmful particles and gases,notably from cigarette smoke.This inflammatory response is orchestrated by innate immune cells like macrophages and epithelial cells,which recognize danger signals released from damaged cells.Prolonged inflammation prompts an adaptive immune reaction mediated by dendritic cells,culminating in the formation of lymphoid follicles and involving a complex interplay of T and B cells,as well as cytotoxic activity.Additionally,both viral and bacterial infections exacerbate COPD by further igniting inflammatory pathways,perpetuating the chronic inflammatory state.This comprehensive review encapsulates the intricate interplay between innate and adaptive immunity in COPD,with a particular focus on the role of cigarette smoke in its pathogenesis and potential therapeutic targets.
基金supported by the National Natural Science Foundation of China(NSFC)(Grant no.31900310 to R.T.)the Key Project of the National Natural Science Foundation of China(NSFC)(Grant no.31630071 to G.Y.)+1 种基金the National Natural Science Foundation of China(NSFC)(Grant no.31950410545 to I.S.)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘The molecular basis for adaptations to extreme environments can now be understood by interrogating the everincreasing number of sequenced genomes.Mammals such as cetaceans,bats,and highland species can protect themselves from oxidative stress,a disruption in the balance of reactive oxygen species,which results in oxidative injury and cell damage.Here,we consider the evolution of the glutathione peroxidase(GPX)family of antioxidant enzymes by interrogating publicly available genome data from 70 mammalian species from all major clades.We identified 8 GPX subclasses ubiquitous to all mammalian groups.Mammalian GPX gene families resolved into the GPX4/7/8 and GPX1/2/3/5/6 groups and are characterized by several instances of gene duplication and loss,indicating a dynamic process of gene birth and death in mammals.Seven of the eight GPX subfamilies(all but GPX7)were under positive selection,with the residues under selection located at or close to active sites or at the dimer interface.We also reveal evidence of a correlation between ecological niches(e.g.high oxidative stress)and the divergent selection and gene copy number of GPX subclasses.Notably,a convergent expansion of GPX1 was observed in several independent lineages of mammals under oxidative stress and may be important for avoiding oxidative damage.Collectively,this study suggests that the GPX gene family has shaped the adaption of mammals to stressful environments.
文摘The adaptive mesh mesh technique is developed and applied in three dimensional numerical simulation of welding process on the base of the commercial software. Special user subroutine is worked out to accom- plish this function.This technique can make the dense mesh moving simultaneously with the heat source while the other area of the structure with much coarser mesh, greatly reducing the number of nodes and elements in the analysis.Temperature field,displacement and stress distributions during welding pro- cess me analyzed by FEM method with adaptive mesh and the analysis is also conducted with normal FEM method. The temperature field,displacement and stress distributions obtained with both methods are shown in contrast. The results show that the temperature fields and the displacement distributions of simulation on adaptive mesh correspond wery well with that of without adaptive mesh. Though the stress distributions have some difference,but the trends of the stress distribution are corresponding.The com- parison of the computing time of the two meshes indicates that the adaptive that the adaptive mesh can greatly reduce the calculation time when used for welding process.
文摘This paper presents the adaptive mesh finite element estimation method for analyzing 2D linear elastic fracture problems. The mesh is generated by the advancing front method and the norm stress error is taken as a posteriori error estimator for the h-type adaptive refinement. The stress intensity factors are estimated by a displacement extrapolation technique. The near crack tip displacements used are obtained from specific nodes of natural six-noded quarter-point elements which are generated around the crack tip defined by the user. The crack growth and its direction are determined by the calculated stress intensity factors. The maximum circumference theory is used for the latter. In evaluating the accuracy of the estimated stress intensity factors, four cases are tested consisting of compact tension specimen, three-point bending specimen, central cracked plate and double edge notched plate. These were carried out and compared to the results from other studies. The crack trajectories of these specimen tests are also illustrated.
基金supported by JSPS KAKENHI Grant Number JP17F17102the US Air Force [AFOSR FA9550-13-1-0047]ExxonMobil Foundation [S18200000000256]
文摘Environmental change attracts particular attention by biologists concerned with the performance of biological systems under stress. To investigate these, dose–response relationships should be clarified. It was previously assumed that the fundamental nature of biological dose–responses follows a linear model, either with no threshold or with a threshold below which no effects are expected to occur in biological endpoints. However, substantial literature, including widespread documentation in plants, has revealed that hormesis commonly occurs. Hormesis is highly generalized and can be utilized as a quantitative measure of biological plasticity. Conditioning induced by adaptive responses also occurs in the framework of hormesis and is of particular importance to environmental change biology with regards to evolutionary adaptations.This paper presents additional evidence for hormetic dose responses induced by temperature in plants. The current understanding on hormesis provides a perspective for next generation environmental change research. Hormesis should have a central role in environmental change biology of vegetation.
