In this study, the detection method of tea saponin has been studied firstly. Determining the maximum absorption wavelength is 540 nm. Standard curve equation is y = 0.0015x - 0.0885. The correlation coefficient r = 0....In this study, the detection method of tea saponin has been studied firstly. Determining the maximum absorption wavelength is 540 nm. Standard curve equation is y = 0.0015x - 0.0885. The correlation coefficient r = 0.9983 (p < 0.01). The relative standard deviation is 1.13%. Reclaimable rate of adding standard sample is 89.5% - 97.7%. Comparing vanille-sulfuric acid of gravimetric determination, the maximum deviation is 3.27%, indicating that vanille-sulfuric acid method is worth of quantitative analysis of tea saponin.展开更多
采用间苯三酚-浓硫酸显色法,建立双波长分光光度法测定溶液中木糖和葡萄糖含量,并对最佳测定条件及溶液中木糖及葡萄糖共存时的相互影响进行了分析,探究其测定木质纤维素酶解液中木糖和葡萄糖含量的可行性。实验结果表明:与间苯三酚-浓...采用间苯三酚-浓硫酸显色法,建立双波长分光光度法测定溶液中木糖和葡萄糖含量,并对最佳测定条件及溶液中木糖及葡萄糖共存时的相互影响进行了分析,探究其测定木质纤维素酶解液中木糖和葡萄糖含量的可行性。实验结果表明:与间苯三酚-浓硫酸显色后,木糖和葡萄糖的最大吸收峰分别为444 nm和420 nm;最佳测定条件为:1.0 m L木糖或葡萄糖标准溶液,加5.0 m L显色剂,100℃水浴加热10 min。木糖和葡萄糖浓度在20～120μg/m L范围内线性关系良好;精密度实验得到木糖和葡萄糖的标准偏差分别为0.8432和0.8430μg/m L,RSD分别为1.3967%和1.3959%;加标回收实验得到木糖和葡萄糖的回收率分别为100.82%和100.65%,RSD分别为0.32%和0.46%。该方法操作简单,精确度高,可用于实验室快速测定溶液中葡萄糖和木糖的含量,但两种单糖共存时对测定结果有一定的影响。展开更多
文摘In this study, the detection method of tea saponin has been studied firstly. Determining the maximum absorption wavelength is 540 nm. Standard curve equation is y = 0.0015x - 0.0885. The correlation coefficient r = 0.9983 (p < 0.01). The relative standard deviation is 1.13%. Reclaimable rate of adding standard sample is 89.5% - 97.7%. Comparing vanille-sulfuric acid of gravimetric determination, the maximum deviation is 3.27%, indicating that vanille-sulfuric acid method is worth of quantitative analysis of tea saponin.
文摘采用间苯三酚-浓硫酸显色法,建立双波长分光光度法测定溶液中木糖和葡萄糖含量,并对最佳测定条件及溶液中木糖及葡萄糖共存时的相互影响进行了分析,探究其测定木质纤维素酶解液中木糖和葡萄糖含量的可行性。实验结果表明:与间苯三酚-浓硫酸显色后,木糖和葡萄糖的最大吸收峰分别为444 nm和420 nm;最佳测定条件为:1.0 m L木糖或葡萄糖标准溶液,加5.0 m L显色剂,100℃水浴加热10 min。木糖和葡萄糖浓度在20～120μg/m L范围内线性关系良好;精密度实验得到木糖和葡萄糖的标准偏差分别为0.8432和0.8430μg/m L,RSD分别为1.3967%和1.3959%;加标回收实验得到木糖和葡萄糖的回收率分别为100.82%和100.65%,RSD分别为0.32%和0.46%。该方法操作简单,精确度高,可用于实验室快速测定溶液中葡萄糖和木糖的含量,但两种单糖共存时对测定结果有一定的影响。
