[Objectives]To identify and analyze chemical components in Zhideke Granules by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS).[M...[Objectives]To identify and analyze chemical components in Zhideke Granules by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS).[Methods]Zhideke Granules were isolated on a Thermo Hypersil Gold C18 column(2.1 mm×100 mm,1.9μm).The mobile phase was 0.1%formic acid acetonitrile and 0.1%formic acid aqueous solution(containing 10 mmol ammonium acetate)with gradient elution.Chemical components in Zhideke Granules were rapidly isolated and identified by HRMS in the positive and negative ion mode with full scan data-dependent two stage threshold-triggered mass modes(Full MS/dd-MS2).[Results]The secondary fragment ion information of the target compound was selected and compared with the compound reported in the databases and related literatures for further confirmation.In total,30 chemical compounds were identified,including 12 flavonoids and glycosides,9 organic acids,3 nitrogen-containing compounds,and 6 other components.[Conclusions]In this study,the UPLC-Q-Orbitrap HRMS was used for the first time to analyze the chemical components in Zhideke Granules.It is intended to provide a reference for the quality evaluation and further study of pharmacodynamic materials of Zhideke Granules.展开更多
[Objectives]To test the microbial limit of Zhideke granules.[Methods]According to the relevant regulations on the testing of granules in Chinese Pharmacopoeia in 2015,the applicability of the methods for counting the ...[Objectives]To test the microbial limit of Zhideke granules.[Methods]According to the relevant regulations on the testing of granules in Chinese Pharmacopoeia in 2015,the applicability of the methods for counting the total number of aerobic bacteria,molds and yeasts and objectionable organisms in Zhideke granules was studied.[Results]The total number of aerobic bacteria in Zhideke granules was determined by dilution plate method(1∶20,1 mL per dish),the total number of molds and yeasts was determined by conventional plate method(1∶10,1 mL per dish),and E.coli was detected by conventional method of TTB(1∶10 test solution,10-100 mL TSB).[Conclusions]The method was suitable for the microbial limit test of Zhideke granules.展开更多
基金Supported by Special Fund Project for Innovation Driven Development of Guangxi(Gui Ke AA17202046)Guangxi One Thousand Young and Middle-Aged College and University Backbones Teachers Cultivation Program(Gui Jiao Ren[2019]No.5)+6 种基金Program of Collaborative Innovation Center of Zhuang and Yao Medicine(Gui Jiao Ke Yan[2013]No.20)Program of Guangxi Province Key Laboratory of Zhuang and Yao Medicine(Gui Ke Ji Zi[2014]No.32)Project for Program of High-level Innovation Teams and Outstanding Scholars of Guangxi Colleges and Universities(Gui Jiao Shi Fan[2019]No.52)The Eighth Batch of Special Experts Project of Guangxi Zhuang Autonomous Region(Study on the Quality Standard of Zhuang and Yao Medicine:Gui Ren Cai Tong Zi[2019]No.13)First-class Discipline in Guangxi-Traditional Chinese Medicine(Gui Jiao Ke Yan[2018]No.12)Guangxi Science and Technology Base and Talent Project(Gui Ke AD20238058)Key Research and Development Program of Nanning City(20193115).
文摘[Objectives]To identify and analyze chemical components in Zhideke Granules by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS).[Methods]Zhideke Granules were isolated on a Thermo Hypersil Gold C18 column(2.1 mm×100 mm,1.9μm).The mobile phase was 0.1%formic acid acetonitrile and 0.1%formic acid aqueous solution(containing 10 mmol ammonium acetate)with gradient elution.Chemical components in Zhideke Granules were rapidly isolated and identified by HRMS in the positive and negative ion mode with full scan data-dependent two stage threshold-triggered mass modes(Full MS/dd-MS2).[Results]The secondary fragment ion information of the target compound was selected and compared with the compound reported in the databases and related literatures for further confirmation.In total,30 chemical compounds were identified,including 12 flavonoids and glycosides,9 organic acids,3 nitrogen-containing compounds,and 6 other components.[Conclusions]In this study,the UPLC-Q-Orbitrap HRMS was used for the first time to analyze the chemical components in Zhideke Granules.It is intended to provide a reference for the quality evaluation and further study of pharmacodynamic materials of Zhideke Granules.
基金Guangxi Special Fund Project for Innovation-driven Development(Gui KeAA17202046)Guangxi 1000 Young and Middle-aged College and University Backbone Teachers Cultivation Program[(2019)5]+8 种基金Guangxi Graduate Education Innovation Program(YCSY20190081)Guangxi College Students Innovation and Entrepreneurship Training Program(201910600091)Guangxi First-class Discipline—Traditional Chinese Medicine(Ethnopharmacology)Project[Gui Jiao Ke Yan(2018)12]Zhuang Yao Medicine Synergetic Innovation Center[Gui Jiao Ke Yan(2013)20]Guangxi Key Laboratory of Zhuang Yao Medicine[Gui Ke Ji Zi(2014)32]High-level Innovation Team and Outstanding Scholars Program of Guangxi Colleges and Universities[Gui Jiao Shi Fan(2019)52]The 8 th Batch of Special Expert Project in Guangxi Zhuang Autonomous Region[Study on the Quality Standard of Zhuang Yao Medicine,Gui Ren Cai Tong Zi(2019)13]"Qihuang Project"—High-level Talent Team Training Project of Guangxi University of Chinese Medicine(2018002)Guangxi Science and Technology Base and Talent Project(Gui KeAD20238058).
文摘[Objectives]To test the microbial limit of Zhideke granules.[Methods]According to the relevant regulations on the testing of granules in Chinese Pharmacopoeia in 2015,the applicability of the methods for counting the total number of aerobic bacteria,molds and yeasts and objectionable organisms in Zhideke granules was studied.[Results]The total number of aerobic bacteria in Zhideke granules was determined by dilution plate method(1∶20,1 mL per dish),the total number of molds and yeasts was determined by conventional plate method(1∶10,1 mL per dish),and E.coli was detected by conventional method of TTB(1∶10 test solution,10-100 mL TSB).[Conclusions]The method was suitable for the microbial limit test of Zhideke granules.
