The purpose of this study was to explore the differentially expressed genes in lymph-node cells(LNC)of lymphomas and reactive lymph node hyperplasia,and to perform an initial bioinformatic analysis on a novel gene,KIA...The purpose of this study was to explore the differentially expressed genes in lymph-node cells(LNC)of lymphomas and reactive lymph node hyperplasia,and to perform an initial bioinformatic analysis on a novel gene,KIAA0372,which is highly expressed in the LNC of lymphomas.mRNA extracted from LNC of lymphomas and reactive lymph node hyperplasia were respectively marked with biotin and hybridized with Gene Expression Chips,resulting in differentially expressed genes.Initial bioinformatic analysis was then performed on a novel gene named KIAA0372,whose function has not yet been explored.Its structure and genomic location,its product’s physical and chemical properties,subcellular localization and functional domains,were also predicted.Further,a systematic evolution analysis was performed on similar proteins from among several species.Using Gene Expression Chips,many differentially expressed genes were uncovered.Efficient bioinformatic analysis has fundamentally determined that KIAA0372 is an extracellular protein which may be involved in TGF-b signaling.Microarray is an efficient and high throughput strategy for detection of differentially expressed genes.And KIAA0372 is thought to be a potential target for tumor research using bioinformatic analysis.展开更多
基金This project was supported by grants from the National Natural Sciences Foundation of China(Grant No.30371657)Postdoctoral Science Foundation of China(No.2005087678).
文摘The purpose of this study was to explore the differentially expressed genes in lymph-node cells(LNC)of lymphomas and reactive lymph node hyperplasia,and to perform an initial bioinformatic analysis on a novel gene,KIAA0372,which is highly expressed in the LNC of lymphomas.mRNA extracted from LNC of lymphomas and reactive lymph node hyperplasia were respectively marked with biotin and hybridized with Gene Expression Chips,resulting in differentially expressed genes.Initial bioinformatic analysis was then performed on a novel gene named KIAA0372,whose function has not yet been explored.Its structure and genomic location,its product’s physical and chemical properties,subcellular localization and functional domains,were also predicted.Further,a systematic evolution analysis was performed on similar proteins from among several species.Using Gene Expression Chips,many differentially expressed genes were uncovered.Efficient bioinformatic analysis has fundamentally determined that KIAA0372 is an extracellular protein which may be involved in TGF-b signaling.Microarray is an efficient and high throughput strategy for detection of differentially expressed genes.And KIAA0372 is thought to be a potential target for tumor research using bioinformatic analysis.
