Yak (Bos grunniens) is one of the largest ungulate in China, found in the central and eastern cold pastures of Qinghai-Tibet Plateau. The number of wild yaks has declined considerably in recent decades due to widespre...Yak (Bos grunniens) is one of the largest ungulate in China, found in the central and eastern cold pastures of Qinghai-Tibet Plateau. The number of wild yaks has declined considerably in recent decades due to widespread hunting. In this paper we discussed the distribution of the Yak, based on the available literature and our field investigation on the Qinghai-Tibet Plasteau, to provide basic knowledge and promote the conservation of this animal.展开更多
以取自四川省不同地区的牦牛粪便、肠道内容物为材料,用MRS琼脂双层培养基进行厌氧培养,分离到50株乳酸菌,经生化鉴定为嗜热链球菌(2株)、乳酸乳球菌(1株)、保加利亚乳杆菌(5株)、嗜粪乳杆菌(10株)、嗜酸乳杆菌(8株)、乳酸乳杆菌(9株)...以取自四川省不同地区的牦牛粪便、肠道内容物为材料,用MRS琼脂双层培养基进行厌氧培养,分离到50株乳酸菌,经生化鉴定为嗜热链球菌(2株)、乳酸乳球菌(1株)、保加利亚乳杆菌(5株)、嗜粪乳杆菌(10株)、嗜酸乳杆菌(8株)、乳酸乳杆菌(9株)、肠乳杆菌(10株)、弯曲乳杆菌(5株)。采用乳酸菌16 S rDNA通用引物,对分离的8种菌的16 S rDNA一段可变区序列进行扩增,均得到大小约470 bp的产物;扩增产物经纯化、测序后与GenBank中标准菌株的核甘酸序列比较,同源性均大于97．5％,同源性分析与生化试验的结果是一致的。证实,牦牛肠道和粪便的乳酸菌较为丰富,且乳杆菌的数量较多,这可能与牦牛复杂的生长环境有关。展开更多
文摘Yak (Bos grunniens) is one of the largest ungulate in China, found in the central and eastern cold pastures of Qinghai-Tibet Plateau. The number of wild yaks has declined considerably in recent decades due to widespread hunting. In this paper we discussed the distribution of the Yak, based on the available literature and our field investigation on the Qinghai-Tibet Plasteau, to provide basic knowledge and promote the conservation of this animal.
文摘以取自四川省不同地区的牦牛粪便、肠道内容物为材料,用MRS琼脂双层培养基进行厌氧培养,分离到50株乳酸菌,经生化鉴定为嗜热链球菌(2株)、乳酸乳球菌(1株)、保加利亚乳杆菌(5株)、嗜粪乳杆菌(10株)、嗜酸乳杆菌(8株)、乳酸乳杆菌(9株)、肠乳杆菌(10株)、弯曲乳杆菌(5株)。采用乳酸菌16 S rDNA通用引物,对分离的8种菌的16 S rDNA一段可变区序列进行扩增,均得到大小约470 bp的产物;扩增产物经纯化、测序后与GenBank中标准菌株的核甘酸序列比较,同源性均大于97．5％,同源性分析与生化试验的结果是一致的。证实,牦牛肠道和粪便的乳酸菌较为丰富,且乳杆菌的数量较多,这可能与牦牛复杂的生长环境有关。
