目的：探讨醒脑静对脑出血急性期患者血清转铁蛋白（Tf）及铁蛋白（Ft）的变化,并观察神经功能改善状况,为评估脑出血急性期治疗提供依据。方法：选择2004年2月—2010年2月医院急性脑出血患者122例,随机分为对照组和治疗组,对照组60例,...目的：探讨醒脑静对脑出血急性期患者血清转铁蛋白（Tf）及铁蛋白（Ft）的变化,并观察神经功能改善状况,为评估脑出血急性期治疗提供依据。方法：选择2004年2月—2010年2月医院急性脑出血患者122例,随机分为对照组和治疗组,对照组60例,进行常规治疗,治疗组62例,在对照组基础上应用醒脑静治疗,选取同期健康体检者30例,为正常组。入院后第1、3、7、14、21天检测血清Tf及Ft水平。采用美国国立卫生院神经功能缺损评分标准（NIHSS）在入院第1、7、14、21天对患者进行神经功能缺损程度评分。结果：治疗组和对照组Tf在第1天高于正常组（P1=0.025,P2=0.031）;对照组Tf在发病第3天高于正常组（P1=0.014）。治疗组Tf在第3天较对照组显著下降（P=0.034）。治疗组和对照组血清Ft在发病后第7天、14天与正常组比较,差异具有统计学意义（P1=0.018,0.025,0.001,0.038）。对照组Ft在第21天高于正常组（P=0.005）;治疗组第7天、第14天血清Ft低于对照组（P1=0.009,P2=0.038）。血量在10~20 m L及≥20 m L时,治疗组血清Tf和Ft均低于对照组,差异具有统计学意义（P1=0.006,0.007;0.021,0.039）。对照组和治疗组第7天、14天、21天NIHSS评分比较,差异有统计学意义（P=0.004,0.002,0.001）。结论：醒脑静注射液可以降低脑出血急性期血清转铁蛋白及铁蛋白水平,改善患者神经缺损程度。展开更多
The aim of this study was to explore the neuroprotective effect and mechanism of XingNaoJing injections(XNJ) on cerebral ischemia injury and blood-brain barrier(BBB) disruption. Middle cerebral artery occlusion(MCAO) ...The aim of this study was to explore the neuroprotective effect and mechanism of XingNaoJing injections(XNJ) on cerebral ischemia injury and blood-brain barrier(BBB) disruption. Middle cerebral artery occlusion(MCAO) method was applicated to establish the model of cerebral ischemia/reperfusion(I/R) injury in rats. BBB permeability after I/R injury was assessed with the leaking amount of Evans Blue and the expression of occludin and ZO-1. The expression of NOD-like receptor family, pyrin domain containing(NLRP3) was checked to explore the inhibition of inflammation by XNJ. The results showed that XNJ could significantly increase the survival percent, decrease the infarct area and ameliorate neurological deficits and brain damage after I/R injury. Leaking amount of Evans Blue was reduced by XNJ, and the expression of tight junction protein, occludin and ZO-1 was also up-regulated by XNJ, which showed a role of protection on BBB disruption. The expression of NLRP3 was inhibited after exposure of XNJ, which was associated with inhibition of the inflammatory response. In summary, XNJ could suppress NLRP3 inflammasomes and improve BBB disruption and brain damage in rats after cerebral I/R injury, which provided a beneficial insight to further explore XNJ.展开更多
文摘目的：探讨醒脑静对脑出血急性期患者血清转铁蛋白（Tf）及铁蛋白（Ft）的变化,并观察神经功能改善状况,为评估脑出血急性期治疗提供依据。方法：选择2004年2月—2010年2月医院急性脑出血患者122例,随机分为对照组和治疗组,对照组60例,进行常规治疗,治疗组62例,在对照组基础上应用醒脑静治疗,选取同期健康体检者30例,为正常组。入院后第1、3、7、14、21天检测血清Tf及Ft水平。采用美国国立卫生院神经功能缺损评分标准（NIHSS）在入院第1、7、14、21天对患者进行神经功能缺损程度评分。结果：治疗组和对照组Tf在第1天高于正常组（P1=0.025,P2=0.031）;对照组Tf在发病第3天高于正常组（P1=0.014）。治疗组Tf在第3天较对照组显著下降（P=0.034）。治疗组和对照组血清Ft在发病后第7天、14天与正常组比较,差异具有统计学意义（P1=0.018,0.025,0.001,0.038）。对照组Ft在第21天高于正常组（P=0.005）;治疗组第7天、第14天血清Ft低于对照组（P1=0.009,P2=0.038）。血量在10~20 m L及≥20 m L时,治疗组血清Tf和Ft均低于对照组,差异具有统计学意义（P1=0.006,0.007;0.021,0.039）。对照组和治疗组第7天、14天、21天NIHSS评分比较,差异有统计学意义（P=0.004,0.002,0.001）。结论：醒脑静注射液可以降低脑出血急性期血清转铁蛋白及铁蛋白水平,改善患者神经缺损程度。
基金surported by the National Natural Science Foundation of China(No.81803608)the Administration of Traditional Chinese Medicine of Jilin Province,China(No.2019133)
文摘The aim of this study was to explore the neuroprotective effect and mechanism of XingNaoJing injections(XNJ) on cerebral ischemia injury and blood-brain barrier(BBB) disruption. Middle cerebral artery occlusion(MCAO) method was applicated to establish the model of cerebral ischemia/reperfusion(I/R) injury in rats. BBB permeability after I/R injury was assessed with the leaking amount of Evans Blue and the expression of occludin and ZO-1. The expression of NOD-like receptor family, pyrin domain containing(NLRP3) was checked to explore the inhibition of inflammation by XNJ. The results showed that XNJ could significantly increase the survival percent, decrease the infarct area and ameliorate neurological deficits and brain damage after I/R injury. Leaking amount of Evans Blue was reduced by XNJ, and the expression of tight junction protein, occludin and ZO-1 was also up-regulated by XNJ, which showed a role of protection on BBB disruption. The expression of NLRP3 was inhibited after exposure of XNJ, which was associated with inhibition of the inflammatory response. In summary, XNJ could suppress NLRP3 inflammasomes and improve BBB disruption and brain damage in rats after cerebral I/R injury, which provided a beneficial insight to further explore XNJ.
