本实验观察了低剂量 X 射线整体和离体照射后对小鼠脾淋巴细胞丝裂原反应的影响。结果表明,X 射线全身照射后小鼠脾脏 T、B 淋巴细胞对刀豆蛋白-A(Con A)和细菌脂多糖(LPS)的反应较对照组增强,尤以75 mGy 组最为明显。在离体照射后,脾...本实验观察了低剂量 X 射线整体和离体照射后对小鼠脾淋巴细胞丝裂原反应的影响。结果表明,X 射线全身照射后小鼠脾脏 T、B 淋巴细胞对刀豆蛋白-A(Con A)和细菌脂多糖(LPS)的反应较对照组增强,尤以75 mGy 组最为明显。在离体照射后,脾淋巴细胞对 Con A 的反应未见明显改变,而对 LPS 反应则低于对照组。提示低剂量 X 射线照射可刺激 T 细胞反应增强。展开更多
AIM： To establish a new, reliable vomit model of minks METHODS： Adult male minks were randomly divided into 8 groups （n=6）： cisplatin （7.5 mg/kg） intraperitoneal injection （ip） group, copper sulfate （40 mg/k...AIM： To establish a new, reliable vomit model of minks METHODS： Adult male minks were randomly divided into 8 groups （n=6）： cisplatin （7.5 mg/kg） intraperitoneal injection （ip） group, copper sulfate （40 mg/kg） intragastric injection （ig） group, apomorphine （1.6 mg/kg） subcutaneous injection （sc） group, and 18 Gy whole-body X-irradiation group, ondansetron injection group （2 mg/kg ip） 30 min later followed by cisplatin （7.5 mg/kg） ip, normal saline （NS） ip injection control group, metoclopramide injection group （4 mg/kg ip） 30 rain later followed by apomorphine （1.6 mg/kg） sc, NS ig control group. The frequency of retching and vomiting was calculated. After behavioral experiment, distribution of 5-HT in the ileum was detected by immunohistologic method. RESULTS： Cisplatin, apomorphine, copper sulfate and X-irradiation administered to minks evoked a profound emetic response in the animals. However, retching and vomiting were significantly inhibited by pretreatment with ondansetron and metoclopramide in cisplatin and copper sulfate groups （P=0.018）. Immunohistologic result showed that 5-HT released from enterochromaffin cells （EC cells） was involved in vomiting mechanism. CONCLUSION： Mink vomit model has a great value in studying the vomiting mechanism and screening new antiemetic drugs.展开更多
X-ray storage phosphors MFX:Eu<sup>2+</sup> （M=Ca, Sr, Ba; X=Cl, Br, I） have been studied extensively, because they exhibit photostimulated luminescence （PSL） properties and offer an alternative to con...X-ray storage phosphors MFX:Eu<sup>2+</sup> （M=Ca, Sr, Ba; X=Cl, Br, I） have been studied extensively, because they exhibit photostimulated luminescence （PSL） properties and offer an alternative to conventional X-ray imaging technology. Color centers play a very important role in the PSL process; much work on the color centers in this kind of materials has been done, such as the formation and optical properties of F centers, V centers and oxygen defects in BaFCl and BaFBr crystals.The study of color centers provides direct evidence for the understanding of the PSL process and is helpful for the practical application of these materials. New color centers and PSL of BaFCl: Eu<sup>2+[8]</sup> and BaFBr: Eu<sup>2+[9]</sup> crystals have been reported. New centers in SrFCl: Eu<sup>2+</sup> and BaFI: Eu<sup>2+</sup>展开更多
X-irradiation has a beneficial effect in treating spinal cord injury. We supposed that X-irradiation could improve the microenvironment at the site of a spinal cord injury and inhibit glial scar formation. Thus, this ...X-irradiation has a beneficial effect in treating spinal cord injury. We supposed that X-irradiation could improve the microenvironment at the site of a spinal cord injury and inhibit glial scar formation. Thus, this study was designed to observe the effects of 8 Gy X-irradiation on the injury site at 6 hours and 2, 4, 7, and 14 days post injury, in terms of improvement in the microenvironment and hind limb motor function. Immunohistochemistry showed that the expression of macrophage marker ED-1 and the area with glial scar formation were reduced. In addition, the Basso, Beattie and Bresnahan score was higher at 7 days post injury relative to the other time points post injury. Results indicated that X-irradiation at a dose of 8 Gy can inhibit glial scar formation and alleviate the inflammatory reaction, thereby repairing spinal cord injury. X-irradiation at 7 days post spinal cord injury may be the best time window.展开更多
Objective To test the hypothesis that delayed X-irradiation can enhance the functional and structural recovery of the injured spinal cord in rats. Methods Seventy Sprague-Dawley rots were randomly divided into two gr...Objective To test the hypothesis that delayed X-irradiation can enhance the functional and structural recovery of the injured spinal cord in rats. Methods Seventy Sprague-Dawley rots were randomly divided into two groups, 35 rats in each. The control group sustained a one-minute clip compression （ force of clip was 30 g） injury of the spinal cord at the T2 level, without X-irradiation. The experimental group received X-irradiation 14 days after injury. Neurological function was assessed by the modified Tarlov method, including hind limbs movement, inclined plane, and pain withdrawal. These tests were performed in a blinded fashion at 3, 7, 14, 21, 28, 35, and 42 days after injury. At 43 days after injury, histological examination of the injured spinal cord was performed following decapitation of the rats. Results Sixty-two rats met the experimental requirements （spinal cord injury was similar）, 32 rats in experimental group and 30 rats in control group. Statistically significant difference was observed between the two groups in hind limbs movement and inclined plane （ P 〈0. 01 ）, but not in the pain withdrawal test. The edema and necrosis areas of injured spinal cords in experimental group were less than those in control group, and axons in experimental group were significantly more than those in control group （P 〈0.01）. Conclusion Delayed X-irradiation following spinal cord injury may enhance functional recovery by improving and restoring structural integrity of the injured spinal cord in rats.展开更多
文摘本实验观察了低剂量 X 射线整体和离体照射后对小鼠脾淋巴细胞丝裂原反应的影响。结果表明,X 射线全身照射后小鼠脾脏 T、B 淋巴细胞对刀豆蛋白-A(Con A)和细菌脂多糖(LPS)的反应较对照组增强,尤以75 mGy 组最为明显。在离体照射后,脾淋巴细胞对 Con A 的反应未见明显改变,而对 LPS 反应则低于对照组。提示低剂量 X 射线照射可刺激 T 细胞反应增强。
基金Supported by the Health Department of Shandong Province,No,1999CA1CBA3
文摘AIM： To establish a new, reliable vomit model of minks METHODS： Adult male minks were randomly divided into 8 groups （n=6）： cisplatin （7.5 mg/kg） intraperitoneal injection （ip） group, copper sulfate （40 mg/kg） intragastric injection （ig） group, apomorphine （1.6 mg/kg） subcutaneous injection （sc） group, and 18 Gy whole-body X-irradiation group, ondansetron injection group （2 mg/kg ip） 30 min later followed by cisplatin （7.5 mg/kg） ip, normal saline （NS） ip injection control group, metoclopramide injection group （4 mg/kg ip） 30 rain later followed by apomorphine （1.6 mg/kg） sc, NS ig control group. The frequency of retching and vomiting was calculated. After behavioral experiment, distribution of 5-HT in the ileum was detected by immunohistologic method. RESULTS： Cisplatin, apomorphine, copper sulfate and X-irradiation administered to minks evoked a profound emetic response in the animals. However, retching and vomiting were significantly inhibited by pretreatment with ondansetron and metoclopramide in cisplatin and copper sulfate groups （P=0.018）. Immunohistologic result showed that 5-HT released from enterochromaffin cells （EC cells） was involved in vomiting mechanism. CONCLUSION： Mink vomit model has a great value in studying the vomiting mechanism and screening new antiemetic drugs.
基金Project supported by the National Natural Science Foundation of China.
文摘X-ray storage phosphors MFX:Eu<sup>2+</sup> （M=Ca, Sr, Ba; X=Cl, Br, I） have been studied extensively, because they exhibit photostimulated luminescence （PSL） properties and offer an alternative to conventional X-ray imaging technology. Color centers play a very important role in the PSL process; much work on the color centers in this kind of materials has been done, such as the formation and optical properties of F centers, V centers and oxygen defects in BaFCl and BaFBr crystals.The study of color centers provides direct evidence for the understanding of the PSL process and is helpful for the practical application of these materials. New color centers and PSL of BaFCl: Eu<sup>2+[8]</sup> and BaFBr: Eu<sup>2+[9]</sup> crystals have been reported. New centers in SrFCl: Eu<sup>2+</sup> and BaFI: Eu<sup>2+</sup>
基金supported by the National Natural Science Foundation of China, No. 81070982, 81201400Tianjin Research Program of Application Foundation and Advanced Technology, No. 10JCZDJC18800, 13JCQNJC11100the Research Foundation of Tianjin Health Bureau, No. 09kz104
文摘X-irradiation has a beneficial effect in treating spinal cord injury. We supposed that X-irradiation could improve the microenvironment at the site of a spinal cord injury and inhibit glial scar formation. Thus, this study was designed to observe the effects of 8 Gy X-irradiation on the injury site at 6 hours and 2, 4, 7, and 14 days post injury, in terms of improvement in the microenvironment and hind limb motor function. Immunohistochemistry showed that the expression of macrophage marker ED-1 and the area with glial scar formation were reduced. In addition, the Basso, Beattie and Bresnahan score was higher at 7 days post injury relative to the other time points post injury. Results indicated that X-irradiation at a dose of 8 Gy can inhibit glial scar formation and alleviate the inflammatory reaction, thereby repairing spinal cord injury. X-irradiation at 7 days post spinal cord injury may be the best time window.
文摘Objective To test the hypothesis that delayed X-irradiation can enhance the functional and structural recovery of the injured spinal cord in rats. Methods Seventy Sprague-Dawley rots were randomly divided into two groups, 35 rats in each. The control group sustained a one-minute clip compression （ force of clip was 30 g） injury of the spinal cord at the T2 level, without X-irradiation. The experimental group received X-irradiation 14 days after injury. Neurological function was assessed by the modified Tarlov method, including hind limbs movement, inclined plane, and pain withdrawal. These tests were performed in a blinded fashion at 3, 7, 14, 21, 28, 35, and 42 days after injury. At 43 days after injury, histological examination of the injured spinal cord was performed following decapitation of the rats. Results Sixty-two rats met the experimental requirements （spinal cord injury was similar）, 32 rats in experimental group and 30 rats in control group. Statistically significant difference was observed between the two groups in hind limbs movement and inclined plane （ P 〈0. 01 ）, but not in the pain withdrawal test. The edema and necrosis areas of injured spinal cords in experimental group were less than those in control group, and axons in experimental group were significantly more than those in control group （P 〈0.01）. Conclusion Delayed X-irradiation following spinal cord injury may enhance functional recovery by improving and restoring structural integrity of the injured spinal cord in rats.
