AIM: To investigate the influence of hepatic arterial blockage on blood perfusion of transplanted cancer in rat liver and the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-1 (MMP...AIM: To investigate the influence of hepatic arterial blockage on blood perfusion of transplanted cancer in rat liver and the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-1 (MMP-1), and to explore the mechanisms involved in transarterial embolization (TAE)-induced metastasis of liver cancer preliminarily. METHODS: Walker 256 carcinosarcoma was transplanted into rat liver to establish the liver cancer model. Hepatic arterial ligation (HAL) was used to block the hepatic arterial blood supply and simulate TAE. Blood perfusion of tumor in control, laparotomy control, and HAL group was analyzed by Hoechst 33342 labeling assay, the serum VEGF level was assayed by ELISA, the expression of VEGF and MMP-1 mRNA was detected by in situ hybridization. RESULTS: Two days after HAL, the number of Hoechst 33342 labeled cells which represent the blood perfusion of tumor directly and hypoxia of tumor indirectly in HAL group decreased significantly compared with that in control group (329+/-29 vs 384+/-19, P【0.01). The serum VEGF level in the HAL group increased significantly as against that of the control group (93 ng.L(-1)+/-44 ng.L(-1) vs 55 ng.L(-1)+/-19 ng.L(-1), P【0.05). The expression of VEGF and MMP-1 mRNA in the tumor tissue of the HAL group increased significantly compared with that of the control and the laparotomy control groups (P【0.05). The blood perfusion data of the tumor, represented by the number of Hoechst 33342 labeled cells, showed a good linear inverse correlation with the serum VEGF level (r=-0.606, P【0.05) and the expression of VEGF mRNA in the tumor tissue ( r =-0.338, P【0.01). CONCLUSION: Blockage of hepatic arterial blood supply results in decreased blood perfusion and increased expression of metastasis-associated genes VEGF and MMP-1 of transplanted liver cancer in rats. Decreased blood perfusion and hypoxia may be the major cause of up-regulated expression of VEGF.展开更多
Cosmological expansion or inflation is mathematically described by the theoretical notion of inverse gravity whose variations are parameterized by a factor that is a function of the distance to which cosmological expa...Cosmological expansion or inflation is mathematically described by the theoretical notion of inverse gravity whose variations are parameterized by a factor that is a function of the distance to which cosmological expansion takes prominence over gravity. This assertion is referred to as the inverse gravity inflationary assertion. Thus, a correction to Newtonian gravitational force is introduced where a parameterized inverse gravity force term is incorporated into the classical Newtonian gravitational force equation where the inverse force term is negligible for distances less than the distance to which cosmological expansion takes prominence over gravity. Conversely, at distances greater than the distance to which cosmological expansion takes prominence over gravity. The inverse gravity term is shown to be dominant generating universal inflation. Gravitational potential energy is thence defined by the integral of the difference (or subtraction) between the conventional Newtonian gravitational force term and the inverse gravity term with respect to radius (r) which allows the formulation, incorporation, and mathematical description to and of gravitational redshift, the Walker-Robertson scale factor, the Robinson-Walker metric, the Klein-Gordon lagrangian, and dark energy and its relationship to the energy of the big bang in terms of the Inverse gravity inflationary assertion. Moreover, the dynamic pressure of the expansion of a cosmological fluid in a homogeneous isotropic universe is mathematically described in terms of the inverse gravity inflationary assertion using the stress-energy tensor for a perfect fluid. Lastly, Einstein’s field equations for the description of an isotropic and homogeneous universe are derived incorporating the mathematics of the inverse gravity inflationary assertion to fully show that the theoretical concept is potentially interwoven into the cosmological structure of the universe.展开更多
基金Science and Technology Development Fund of Shanghai Municipality,No.004119086
文摘AIM: To investigate the influence of hepatic arterial blockage on blood perfusion of transplanted cancer in rat liver and the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-1 (MMP-1), and to explore the mechanisms involved in transarterial embolization (TAE)-induced metastasis of liver cancer preliminarily. METHODS: Walker 256 carcinosarcoma was transplanted into rat liver to establish the liver cancer model. Hepatic arterial ligation (HAL) was used to block the hepatic arterial blood supply and simulate TAE. Blood perfusion of tumor in control, laparotomy control, and HAL group was analyzed by Hoechst 33342 labeling assay, the serum VEGF level was assayed by ELISA, the expression of VEGF and MMP-1 mRNA was detected by in situ hybridization. RESULTS: Two days after HAL, the number of Hoechst 33342 labeled cells which represent the blood perfusion of tumor directly and hypoxia of tumor indirectly in HAL group decreased significantly compared with that in control group (329+/-29 vs 384+/-19, P【0.01). The serum VEGF level in the HAL group increased significantly as against that of the control group (93 ng.L(-1)+/-44 ng.L(-1) vs 55 ng.L(-1)+/-19 ng.L(-1), P【0.05). The expression of VEGF and MMP-1 mRNA in the tumor tissue of the HAL group increased significantly compared with that of the control and the laparotomy control groups (P【0.05). The blood perfusion data of the tumor, represented by the number of Hoechst 33342 labeled cells, showed a good linear inverse correlation with the serum VEGF level (r=-0.606, P【0.05) and the expression of VEGF mRNA in the tumor tissue ( r =-0.338, P【0.01). CONCLUSION: Blockage of hepatic arterial blood supply results in decreased blood perfusion and increased expression of metastasis-associated genes VEGF and MMP-1 of transplanted liver cancer in rats. Decreased blood perfusion and hypoxia may be the major cause of up-regulated expression of VEGF.
文摘Cosmological expansion or inflation is mathematically described by the theoretical notion of inverse gravity whose variations are parameterized by a factor that is a function of the distance to which cosmological expansion takes prominence over gravity. This assertion is referred to as the inverse gravity inflationary assertion. Thus, a correction to Newtonian gravitational force is introduced where a parameterized inverse gravity force term is incorporated into the classical Newtonian gravitational force equation where the inverse force term is negligible for distances less than the distance to which cosmological expansion takes prominence over gravity. Conversely, at distances greater than the distance to which cosmological expansion takes prominence over gravity. The inverse gravity term is shown to be dominant generating universal inflation. Gravitational potential energy is thence defined by the integral of the difference (or subtraction) between the conventional Newtonian gravitational force term and the inverse gravity term with respect to radius (r) which allows the formulation, incorporation, and mathematical description to and of gravitational redshift, the Walker-Robertson scale factor, the Robinson-Walker metric, the Klein-Gordon lagrangian, and dark energy and its relationship to the energy of the big bang in terms of the Inverse gravity inflationary assertion. Moreover, the dynamic pressure of the expansion of a cosmological fluid in a homogeneous isotropic universe is mathematically described in terms of the inverse gravity inflationary assertion using the stress-energy tensor for a perfect fluid. Lastly, Einstein’s field equations for the description of an isotropic and homogeneous universe are derived incorporating the mathematics of the inverse gravity inflationary assertion to fully show that the theoretical concept is potentially interwoven into the cosmological structure of the universe.
