目的:建立草莓中甲型肝炎病毒(hapatitis A virus,HAV)的有效富集方法及病毒RNA提取方法,用于草莓中HAV检测。方法:利用甲肝减毒疫苗对已知阴性的草莓样品进行人工污染,通过有效富集条件的优化及RNA提取后,采用实时荧光逆转录-聚合酶链...目的:建立草莓中甲型肝炎病毒(hapatitis A virus,HAV)的有效富集方法及病毒RNA提取方法,用于草莓中HAV检测。方法:利用甲肝减毒疫苗对已知阴性的草莓样品进行人工污染,通过有效富集条件的优化及RNA提取后,采用实时荧光逆转录-聚合酶链式反应进行检测。结果:病毒富集选择Tris-甘氨酸-1 g/100 m L牛肉浸提物缓冲液洗脱、果胶酶30 U、氯仿-正丁醇为抑制剂去除剂、聚乙二醇沉淀、5℃孵育1 h等优化条件,检测灵敏度较高;最优RNA提取方法为美国ABI公司生产的RNA提取试剂盒。采用优化后的方法对草莓样品中HAV病毒的检测显示,该病毒粒子的检测灵敏度可以达31.36 CCID50/20 g样品。同时对50份送检样品进行检测,结果均为阴性。结论:所建立的病毒富集方法和核酸提取方法更适合草莓样品中HAV的检测,灵敏度较高。展开更多
In the wake of the largest‐ever recorded outbreak of mpox in terms of magnitude and geographical spread in human history since May 2022,we innovatively developed an automated online sewage virus enrichment and concen...In the wake of the largest‐ever recorded outbreak of mpox in terms of magnitude and geographical spread in human history since May 2022,we innovatively developed an automated online sewage virus enrichment and concentration robot for disease tracking.Coupled with an artificial intelligence(AI)model,our research aims to estimate mpox cases based on the concentration of the monkeypox virus(MPXV)in wastewater.Our research has revealed a compelling link between the levels of MPXV in wastewater and the number of clinically confirmed mpox infections,a finding that is reinforced by the ability of our AI prediction model to forecast cases with remarkable precision,capturing 87%of the data’s variability.However,it is worth noting that this high precision in predictions may be related to the relatively high frequency of data acquisition and the relatively non‐mobile isolated environment of the hospital itself.In conclusion,this study represents a significant step forward in our ability to track and respond to mpox outbreaks.It has the potential to revolutionize public health surveillance by utilizing innovative technologies for disease surveillance and prediction。展开更多
目的测试并对比反渗透装置与实验室常见的两种水体富集设备Milliflex Plus Pump以及CPS微生物快速富集系统对水体中病毒的富集效率及其对实际样品的富集检测能力。方法以脊髓灰质炎灭活病毒疫苗与手足口灭活病毒疫苗以及海南省各个地区...目的测试并对比反渗透装置与实验室常见的两种水体富集设备Milliflex Plus Pump以及CPS微生物快速富集系统对水体中病毒的富集效率及其对实际样品的富集检测能力。方法以脊髓灰质炎灭活病毒疫苗与手足口灭活病毒疫苗以及海南省各个地区所采集的水样为样本,使用3种富集设备分别对待测样品进行富集浓缩,而后通过实时荧光定量PCR实验测定富集结果并进行比对,同时对其富集时间、成本、耗材以及适用场景等因素进行分析。结果反渗透装置、Milliflex Plus Pump与CPS微生物快速富集系统3种方案对脊髓灰质炎病毒以及手足口病毒的平均富集效率分别为22.31%、22.67%、12.07%,并均可用于实际水样的病毒检测。结论反渗透富集法富集速度快,无使用场地限制,可适用于现场快速检测。展开更多
文摘目的:建立草莓中甲型肝炎病毒(hapatitis A virus,HAV)的有效富集方法及病毒RNA提取方法,用于草莓中HAV检测。方法:利用甲肝减毒疫苗对已知阴性的草莓样品进行人工污染,通过有效富集条件的优化及RNA提取后,采用实时荧光逆转录-聚合酶链式反应进行检测。结果:病毒富集选择Tris-甘氨酸-1 g/100 m L牛肉浸提物缓冲液洗脱、果胶酶30 U、氯仿-正丁醇为抑制剂去除剂、聚乙二醇沉淀、5℃孵育1 h等优化条件,检测灵敏度较高;最优RNA提取方法为美国ABI公司生产的RNA提取试剂盒。采用优化后的方法对草莓样品中HAV病毒的检测显示,该病毒粒子的检测灵敏度可以达31.36 CCID50/20 g样品。同时对50份送检样品进行检测,结果均为阴性。结论:所建立的病毒富集方法和核酸提取方法更适合草莓样品中HAV的检测,灵敏度较高。
基金supported by National Key Research and Development Program of China(2023YFC3041500)Shenzhen Medical Research Funding(D2301014)+2 种基金Shenzhen High‐level Hospital Construction Fund(23250G1001,XKJS‐CRGRK‐005)Shenzhen Clinical Research Center for Emerging Infectious Diseases(No.LCYSSQ20220823091203007)The Science and Technology Innovation and Entrepreneurship Special Foundation of Shenzhen(JSGG20220226090203006).
文摘In the wake of the largest‐ever recorded outbreak of mpox in terms of magnitude and geographical spread in human history since May 2022,we innovatively developed an automated online sewage virus enrichment and concentration robot for disease tracking.Coupled with an artificial intelligence(AI)model,our research aims to estimate mpox cases based on the concentration of the monkeypox virus(MPXV)in wastewater.Our research has revealed a compelling link between the levels of MPXV in wastewater and the number of clinically confirmed mpox infections,a finding that is reinforced by the ability of our AI prediction model to forecast cases with remarkable precision,capturing 87%of the data’s variability.However,it is worth noting that this high precision in predictions may be related to the relatively high frequency of data acquisition and the relatively non‐mobile isolated environment of the hospital itself.In conclusion,this study represents a significant step forward in our ability to track and respond to mpox outbreaks.It has the potential to revolutionize public health surveillance by utilizing innovative technologies for disease surveillance and prediction。
文摘目的运用生物信息学方法探讨甲型流感病毒所致肺损伤的可能机制。方法从Gene Expression Ominbus(GEO)数据库中选择基因表达谱GSE57455、GSE63786和GSE64800,通过GEO2R工具在线分析,筛选出差异表达基因(DEGs),基于STRING构建蛋白互作网络图,筛选出显著模块;并对获取的DEGs进行Gene Ontology(GO)分析和Kyoto Encyclopedia of Genes and Genomes(KEGG)通路富集分析。通过Cystoscape软件筛选出具有高度连接性的关键基因,并构建生物学功能图。结果筛选得到119个交集DEGs,其中上调基因有106个,下调基因13个。GO分析显示,DEGs显著富集于免疫应答、细胞分裂等生物学过程中;显著富集于细胞外间隙、胞外区部分等细胞组成上;显著富集于趋化因子活性、细胞因子受体结合等分子功能上。KEGG通路富集显示,DEGs显著富集于细胞因子及受体相互作用信号通路、趋化因子等信号通路。同时,共筛选得到7个关键基因:FIGNL1、GBP5、ICOS、ARG1、IRF7、CYSLTR1和SLMAF8,其富集作用主要在免疫应答、精氨酸代谢、B细胞反应等生物学功能。结论FIGNL1介导的肺组织细胞受损、IRF7和GBP5介导的Ⅰ型IFN反应、ARG1介导的精氨酸代谢以及ICOS、CYSLTR1和SLAMF8介导的趋化因子和炎性因子的活化与甲型流感病毒所导致的肺损伤密切相关。
文摘目的测试并对比反渗透装置与实验室常见的两种水体富集设备Milliflex Plus Pump以及CPS微生物快速富集系统对水体中病毒的富集效率及其对实际样品的富集检测能力。方法以脊髓灰质炎灭活病毒疫苗与手足口灭活病毒疫苗以及海南省各个地区所采集的水样为样本,使用3种富集设备分别对待测样品进行富集浓缩,而后通过实时荧光定量PCR实验测定富集结果并进行比对,同时对其富集时间、成本、耗材以及适用场景等因素进行分析。结果反渗透装置、Milliflex Plus Pump与CPS微生物快速富集系统3种方案对脊髓灰质炎病毒以及手足口病毒的平均富集效率分别为22.31%、22.67%、12.07%,并均可用于实际水样的病毒检测。结论反渗透富集法富集速度快,无使用场地限制,可适用于现场快速检测。
