为研究青海弧菌的发光机制,参考霍乱弧菌的发光基因,设计了青海弧菌Lux AB基因的扩增引物,经PCR反应得到相应的片段,并建立了与其他常见荧光素酶的系统发育关系;同时对青海弧菌的16S r DNA进行克隆和测序,并基于16S r DNA建立青海弧菌...为研究青海弧菌的发光机制,参考霍乱弧菌的发光基因,设计了青海弧菌Lux AB基因的扩增引物,经PCR反应得到相应的片段,并建立了与其他常见荧光素酶的系统发育关系;同时对青海弧菌的16S r DNA进行克隆和测序,并基于16S r DNA建立青海弧菌与其他相近细菌之间的亲缘关系;随后,成功将Lux AB克隆到表达载体PHSG396中,转化大肠杆菌Top10,成功实现重组质粒在大肠杆菌中的表达。展开更多
In China, water pollution by pesticide mixtures has constituted a serious environmental problem due to potential toxicity and bioaccumulation. But few pesticide combinations have exactly similar and dissimilar mechani...In China, water pollution by pesticide mixtures has constituted a serious environmental problem due to potential toxicity and bioaccumulation. But few pesticide combinations have exactly similar and dissimilar mechanisms of action. For this purpose, in tests with the freshwater luminescent bacterium （Vibrio qinghaiensis sp.-Q67）, ten pesticides, including three herbicides and seven insecticides, were selected as test substances. Concentration response analysis was performed for ten individual substances, and for mixtures containing all ten substances in twelve different concentration ratios （based on UDCR and EECR methods）. The observed mixture toxicity was compared with predictions by the two models, concentration addition （CA） and independent action （IA）. The toxicity of the tested mixtures showed a good agreement with those predicted by the concept of CA except four UDCR mixtures： UD10-2, UD10-4, UD10-8 and UD10-10. To examine the influence of imidacloprid in the four UDCR mixtures （UD10-2, UD10-4, UD10-8, UD10-10）, it was removed from the ten-pesticide mixtures and the remaining nine chemicals were combined at the same relative proportions based on the UDCR method （UD9-2, UD9-4, UD9-8, UD9-10）. There was not significant departure from CA for the scattered points with the nine remaining pesticides omitting imidacloprid. Thus, imidacloprid may significantly influence the other pesticides due to its properties.展开更多
文摘为研究青海弧菌的发光机制,参考霍乱弧菌的发光基因,设计了青海弧菌Lux AB基因的扩增引物,经PCR反应得到相应的片段,并建立了与其他常见荧光素酶的系统发育关系;同时对青海弧菌的16S r DNA进行克隆和测序,并基于16S r DNA建立青海弧菌与其他相近细菌之间的亲缘关系;随后,成功将Lux AB克隆到表达载体PHSG396中,转化大肠杆菌Top10,成功实现重组质粒在大肠杆菌中的表达。
基金supported by the National Natural Science Foundation of China (No. 20577023, 20777056)the National Hi-Tech Research and Development Program(863) of China (No. 2007AA06Z417)the Foundation of National Excellent Doctoral Dissertation of China (No.200355)
文摘In China, water pollution by pesticide mixtures has constituted a serious environmental problem due to potential toxicity and bioaccumulation. But few pesticide combinations have exactly similar and dissimilar mechanisms of action. For this purpose, in tests with the freshwater luminescent bacterium （Vibrio qinghaiensis sp.-Q67）, ten pesticides, including three herbicides and seven insecticides, were selected as test substances. Concentration response analysis was performed for ten individual substances, and for mixtures containing all ten substances in twelve different concentration ratios （based on UDCR and EECR methods）. The observed mixture toxicity was compared with predictions by the two models, concentration addition （CA） and independent action （IA）. The toxicity of the tested mixtures showed a good agreement with those predicted by the concept of CA except four UDCR mixtures： UD10-2, UD10-4, UD10-8 and UD10-10. To examine the influence of imidacloprid in the four UDCR mixtures （UD10-2, UD10-4, UD10-8, UD10-10）, it was removed from the ten-pesticide mixtures and the remaining nine chemicals were combined at the same relative proportions based on the UDCR method （UD9-2, UD9-4, UD9-8, UD9-10）. There was not significant departure from CA for the scattered points with the nine remaining pesticides omitting imidacloprid. Thus, imidacloprid may significantly influence the other pesticides due to its properties.
