To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete ...To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete sequence of the virion protein 1(VP1) gene of nine virulent DHAV-1 strains,which were isolated from infected ducklings with clinical symptoms in Shandong province of China in 2007-2008,were tested.The fifth generation duck embryo allantoic liquids of the 9 isolates were tested on 12-day-old duck embryos and on 7-day-old ducklings for the median embryonal lethal doses(ELD 50 s) and the median lethal doses(LD 50 s),respectively.The results showed that the ELD 50 s of embryonic duck eggs of the 9 DHAV-1 isolates were between 1.9 × 10 6 /mL to 1.44 × 10 7 /mL,while the LD 50 s were 2.39 × 10 5 /mL to 6.15 × 10 6 /mL.Cross-neutralization tests revealed that the 9 DHAV-1 isolates were completely neutralized by the standard serum and the hyperimmune sera against the 9 DHAV-1 isolates,respectively.Compared with other virulent,moderate virulent,attenuated vaccine and mild strains,the VP1 genes of the 9 strains shared 89.8%-99.7% similarity at the nucleotide level and 92.4%-99.6% at amino acid level with other DHAV-1 strains.There were three hypervariable regions at the C-terminus(aa 158-160,180-193 and 205-219) and other variable points in VP1 protein,but which didn't cause virulence of DHAV-1 change.展开更多
目的探讨颅脑磁共振成像在帕金森病(Parkinson's disease,PD)与血管性帕金森综合征(vascular Parkinson syndrome,VP)鉴别诊断中的价值。方法选取42例确诊PD和46例确诊VP患者为受试对象,分别纳入PD组和VP组;将同期入院体检的50例健...目的探讨颅脑磁共振成像在帕金森病(Parkinson's disease,PD)与血管性帕金森综合征(vascular Parkinson syndrome,VP)鉴别诊断中的价值。方法选取42例确诊PD和46例确诊VP患者为受试对象,分别纳入PD组和VP组;将同期入院体检的50例健康志愿者纳入对照组。所有受试者均接受颅脑MRI检查。记录三组患者黑质致密带(substantia nigra zona compacta,SNc)宽度(width of pars compacta of substantia nigra,WPCSN)值、中脑直径及两者比值检测结果差异,分析PD患者及VP患者颅脑MRI影像特征。结果三组受试者中脑直径对比差异无统计学意义(P>0.05);PD组WPCSN值及WPCSN值/中脑直径检测结果均显著低于VP组和对照组(P<0.05);VP组和对照组上述两项指标检测结果对比差异无统计学意义(P>0.05)。PD组颅脑MRI影像示无特殊改变或颅脑正常老化改变者居多,VP组颅脑MRI影像多示颅内缺血性改变异常信号,主要区域为皮质下、侧脑室旁脑白质及基底节区等,小脑及脑干累及病例较少。结论颅脑MRI可在PD和VP鉴别诊断中发挥积极作用,具有较高的应用价值。展开更多
基金the Chinese National Natural Sciences Foundation(30871878)Shandong Province Higher Educational Science and Technology Program(J08LF07)the Science and Technology Commission of Shandong Province(2010GNC10914),China
文摘To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete sequence of the virion protein 1(VP1) gene of nine virulent DHAV-1 strains,which were isolated from infected ducklings with clinical symptoms in Shandong province of China in 2007-2008,were tested.The fifth generation duck embryo allantoic liquids of the 9 isolates were tested on 12-day-old duck embryos and on 7-day-old ducklings for the median embryonal lethal doses(ELD 50 s) and the median lethal doses(LD 50 s),respectively.The results showed that the ELD 50 s of embryonic duck eggs of the 9 DHAV-1 isolates were between 1.9 × 10 6 /mL to 1.44 × 10 7 /mL,while the LD 50 s were 2.39 × 10 5 /mL to 6.15 × 10 6 /mL.Cross-neutralization tests revealed that the 9 DHAV-1 isolates were completely neutralized by the standard serum and the hyperimmune sera against the 9 DHAV-1 isolates,respectively.Compared with other virulent,moderate virulent,attenuated vaccine and mild strains,the VP1 genes of the 9 strains shared 89.8%-99.7% similarity at the nucleotide level and 92.4%-99.6% at amino acid level with other DHAV-1 strains.There were three hypervariable regions at the C-terminus(aa 158-160,180-193 and 205-219) and other variable points in VP1 protein,but which didn't cause virulence of DHAV-1 change.
文摘目的探讨颅脑磁共振成像在帕金森病(Parkinson's disease,PD)与血管性帕金森综合征(vascular Parkinson syndrome,VP)鉴别诊断中的价值。方法选取42例确诊PD和46例确诊VP患者为受试对象,分别纳入PD组和VP组;将同期入院体检的50例健康志愿者纳入对照组。所有受试者均接受颅脑MRI检查。记录三组患者黑质致密带(substantia nigra zona compacta,SNc)宽度(width of pars compacta of substantia nigra,WPCSN)值、中脑直径及两者比值检测结果差异,分析PD患者及VP患者颅脑MRI影像特征。结果三组受试者中脑直径对比差异无统计学意义(P>0.05);PD组WPCSN值及WPCSN值/中脑直径检测结果均显著低于VP组和对照组(P<0.05);VP组和对照组上述两项指标检测结果对比差异无统计学意义(P>0.05)。PD组颅脑MRI影像示无特殊改变或颅脑正常老化改变者居多,VP组颅脑MRI影像多示颅内缺血性改变异常信号,主要区域为皮质下、侧脑室旁脑白质及基底节区等,小脑及脑干累及病例较少。结论颅脑MRI可在PD和VP鉴别诊断中发挥积极作用,具有较高的应用价值。
