Background: The aim of this study was to investigate the intergeneric transfer of vancomycin resistance gene vanA between probiotic enterococci in the fermentation progress of soybean meal and in the digestive tract o...Background: The aim of this study was to investigate the intergeneric transfer of vancomycin resistance gene vanA between probiotic enterococci in the fermentation progress of soybean meal and in the digestive tract of growing pigs.One vanA genotype vancomycin resistant E.faecium strain,Efm4,and one chloramphenicol-resistant E.faecalis strain,Efs2,were isolated from twenty-nine probiotic basis feed material/additive samples.For in vitro conjugation,Efm4 and Efs2 were used as starter to ferment soybean meal.For in vivo conjugation,thirty growing pigs were randomly assigned to five groups(n = 6),treated with a basic diet,or supplemented with 10% fermented soybean meal,1% Efm4,5% Efs2 or a combination of 1% Efm4 + 5% Efs2 for 7 d,respectively.Fecal samples of pigs in each group were collected daily for the isolation and dynamic analysis of Efm4,Efs2 and transconjugants.The sequence types(STs) of Efm4,Efs2 and transconjugants were analyzed by multilocus sequence typing(MLST).The vanA harboring plasmid in Efm4 and transconjugants was analyzed by S1-pulsed field gel electrophoresis(PFGE)and further verified by multiple alignments.Results: The results showed that,in FSBM,transconjugants were detected 1 h after the fermentation,with a conjugation frequency of ~ 10^-3 transconjugants/recipient.Transconjugants proliferated with Efm4 and Efs2 in the first 8 h and maintained steadily for 10 d till the end of the experiment.Additionally,in vivo experiment showed that transcojugants were recovered in one of six pigs in both FSBM and Efm4 + Efs2 groups,with conjugation frequency of ~ 10^-5 and ~ 10^-4,respectively.MLST revealed the ST of Efm4,Efs2 and transconjugants was ST1014,ST69 and ST69,respectively.S1-PFGE confirmed the existence of the vanA-harboring,142,988-bp plasmid,which was also a multi-drug resistant plasmid containing Tn1546-like transposon.Conclusions: The findings revealed the potential safety hazard existing in the commercial probiotic enterococci in China,because the horizontal transfer from farm to fork could展开更多
Environmental dissemination of antimicrobial resistance genes may occur through agricultural residues, such as animal manure. We studied the resistome of 16 pool samples of animal manure (pig slurry [n = 8] and poultr...Environmental dissemination of antimicrobial resistance genes may occur through agricultural residues, such as animal manure. We studied the resistome of 16 pool samples of animal manure (pig slurry [n = 8] and poultry manure [n = 8]), and 16 soil samples (manure-amended [n = 8] and nonmanure-amended [n = 8]). All samples were collected in central Spain. Detection was based on 18 selected antimicrobial resistance genes (ARGs). The most commonly detected genes in animal manure were sul1 (16/16), sul2 (16/16), tet(A) (16/16), aadA (16/16), tet(B) (15/16), and str (15/16). Genes blaTEM (7/8), mecA (6/8), vanA (5/8) and qnrB (4/8) were more frequently detected in chicken manure, whereas pig slurry samples presented higher levels of tet(C) (8/8) and tet(M) (8/8). Out of the four genes selected for their clinical relevance, three—blaCTX-M, vanA, and mecA—were detected in animal manure. The blaCTX-M (1/8) and vanA (5/8) genes were only identified in chicken manure. To our knowledge, this is the first report of direct detection of mecA gene in poultry manure and pig slurry. Eleven out of 18 ARGs were detected in amended soil, while only genes sul2 (3/8) and str (2/8) were found in nonanthropogenically impacted soils (NAIS), supporting the hypothesis that ARGs may serve as indicators of “anthropogenic impact” on the environment.展开更多
Objective To study the techniques and clinical applications of intra-vascular stenting in the treatment of superior vana cava obliteration syndrome (SVS). Methods In 9 cases of SVS, primary lesions were all confirme...Objective To study the techniques and clinical applications of intra-vascular stenting in the treatment of superior vana cava obliteration syndrome (SVS). Methods In 9 cases of SVS, primary lesions were all confirmed as malignancy (primary pulmonary carcinoma of right upper lobe) histolo-pathologically. By route of right femoral vein, SVS catheterization and DSA was made. The length of the strictures and the diameters of normal superior vana cavae (SVC) were measured for the choice of appropriate stents. The option of stemt diameter is 10% larger than that of normal SVCs. The upper and lower ends of the stent should be 1–2 cm protruding from the ends of the stricture. The stent was dilated with a balloon after its successful placement. Therapy of original lesions was continued together with anticoagulant. Stents were observed about their positions by fluoroscopy or chest films, and about patency of SVC by Doppler. Results After the placement of a stent, DSA revealed the contrast media in the SVCs passed along smoothly, diameters of SVCs almost normal, collateral branches diminished remarkably. Average SVC pressure was decreased from 26.4 cmH2O before the placement down to 15.7 cmH2O, with an obvious difference (P<0.01= by statistics. Related clinical symptoms and signs disappeared or relieved. Subsidised were swelling of head and neck, upper extremities and chest. Excretion of urine increased. Gorgeous superficial veins in the chest could not be detected any more. Conclusion The therapy of intra-vascular stenting to treat SVS is microinvasive, simple and effective. Key words superior vana cava syndrome - tumor - stent - image diagnosis - interventional therapy展开更多
Objective:To investigate the species prevalence of Enterococcus with their antimicrobial resistance pattern from patients of Dhaka Medical College Hospital.Methods:Samples were cultured and Enterococcus species were i...Objective:To investigate the species prevalence of Enterococcus with their antimicrobial resistance pattern from patients of Dhaka Medical College Hospital.Methods:Samples were cultured and Enterococcus species were identified by conventional biochemical tests as well as PCR by using species specific primers for Enterococcus faecalis(E.faecalis)and Enterococcus faecium(E.faecium).For isolation of vancomycin resistant enterococci,minimum inhibitory concentration of vancomycin and PCR was done to detect vanA and vanB genes.Results:A total of 16 enterococci were isolated from 300 urine and 200 wound swab samples(15 from urine and 1 from wound swab)from July 2011 to June 2012.Enterococci were the third most common organism(8.47%)from urine after Escherichia coli(63.28%)and Enterobacter(11.87%).Out of 16 enterococci,10(62.5%)were E.faecalis,4(25%)were E.faecium and 2(12.5%)were other species.All the enterococci(100%)were sensitive to vancomycin and linezolid.Most of the strains were resistant to ciprofloxacin and azithromycin(87.5%),gentamycin(81.25%),ceftriaxone(75%),amoxiclav(31.25%)and imipenem(25%).E.faecium was more resistant than E.faecalis to azithromycin(100%),ciprofloxacin(100%),amoxiclav(75%)and imipenem(50%).No vancomycin resistant enterococci were identified and the range of minimum inhibitory concentration for vancomycin was 1-4μg/mL.None of the enterococci were positive for vanA and vanB genes.Conclusions:The presence of multidrug resistant enterococci should be considered as danger alarm for serious enterococcal infections and further study in large scale is needed.展开更多
基金funded by National Key Research and Development Program of China(Grant number 2016YFD0501308)Agro-scientific Research in the Public Interest(Grant number 201403047)
文摘Background: The aim of this study was to investigate the intergeneric transfer of vancomycin resistance gene vanA between probiotic enterococci in the fermentation progress of soybean meal and in the digestive tract of growing pigs.One vanA genotype vancomycin resistant E.faecium strain,Efm4,and one chloramphenicol-resistant E.faecalis strain,Efs2,were isolated from twenty-nine probiotic basis feed material/additive samples.For in vitro conjugation,Efm4 and Efs2 were used as starter to ferment soybean meal.For in vivo conjugation,thirty growing pigs were randomly assigned to five groups(n = 6),treated with a basic diet,or supplemented with 10% fermented soybean meal,1% Efm4,5% Efs2 or a combination of 1% Efm4 + 5% Efs2 for 7 d,respectively.Fecal samples of pigs in each group were collected daily for the isolation and dynamic analysis of Efm4,Efs2 and transconjugants.The sequence types(STs) of Efm4,Efs2 and transconjugants were analyzed by multilocus sequence typing(MLST).The vanA harboring plasmid in Efm4 and transconjugants was analyzed by S1-pulsed field gel electrophoresis(PFGE)and further verified by multiple alignments.Results: The results showed that,in FSBM,transconjugants were detected 1 h after the fermentation,with a conjugation frequency of ~ 10^-3 transconjugants/recipient.Transconjugants proliferated with Efm4 and Efs2 in the first 8 h and maintained steadily for 10 d till the end of the experiment.Additionally,in vivo experiment showed that transcojugants were recovered in one of six pigs in both FSBM and Efm4 + Efs2 groups,with conjugation frequency of ~ 10^-5 and ~ 10^-4,respectively.MLST revealed the ST of Efm4,Efs2 and transconjugants was ST1014,ST69 and ST69,respectively.S1-PFGE confirmed the existence of the vanA-harboring,142,988-bp plasmid,which was also a multi-drug resistant plasmid containing Tn1546-like transposon.Conclusions: The findings revealed the potential safety hazard existing in the commercial probiotic enterococci in China,because the horizontal transfer from farm to fork could
文摘Environmental dissemination of antimicrobial resistance genes may occur through agricultural residues, such as animal manure. We studied the resistome of 16 pool samples of animal manure (pig slurry [n = 8] and poultry manure [n = 8]), and 16 soil samples (manure-amended [n = 8] and nonmanure-amended [n = 8]). All samples were collected in central Spain. Detection was based on 18 selected antimicrobial resistance genes (ARGs). The most commonly detected genes in animal manure were sul1 (16/16), sul2 (16/16), tet(A) (16/16), aadA (16/16), tet(B) (15/16), and str (15/16). Genes blaTEM (7/8), mecA (6/8), vanA (5/8) and qnrB (4/8) were more frequently detected in chicken manure, whereas pig slurry samples presented higher levels of tet(C) (8/8) and tet(M) (8/8). Out of the four genes selected for their clinical relevance, three—blaCTX-M, vanA, and mecA—were detected in animal manure. The blaCTX-M (1/8) and vanA (5/8) genes were only identified in chicken manure. To our knowledge, this is the first report of direct detection of mecA gene in poultry manure and pig slurry. Eleven out of 18 ARGs were detected in amended soil, while only genes sul2 (3/8) and str (2/8) were found in nonanthropogenically impacted soils (NAIS), supporting the hypothesis that ARGs may serve as indicators of “anthropogenic impact” on the environment.
文摘Objective To study the techniques and clinical applications of intra-vascular stenting in the treatment of superior vana cava obliteration syndrome (SVS). Methods In 9 cases of SVS, primary lesions were all confirmed as malignancy (primary pulmonary carcinoma of right upper lobe) histolo-pathologically. By route of right femoral vein, SVS catheterization and DSA was made. The length of the strictures and the diameters of normal superior vana cavae (SVC) were measured for the choice of appropriate stents. The option of stemt diameter is 10% larger than that of normal SVCs. The upper and lower ends of the stent should be 1–2 cm protruding from the ends of the stricture. The stent was dilated with a balloon after its successful placement. Therapy of original lesions was continued together with anticoagulant. Stents were observed about their positions by fluoroscopy or chest films, and about patency of SVC by Doppler. Results After the placement of a stent, DSA revealed the contrast media in the SVCs passed along smoothly, diameters of SVCs almost normal, collateral branches diminished remarkably. Average SVC pressure was decreased from 26.4 cmH2O before the placement down to 15.7 cmH2O, with an obvious difference (P<0.01= by statistics. Related clinical symptoms and signs disappeared or relieved. Subsidised were swelling of head and neck, upper extremities and chest. Excretion of urine increased. Gorgeous superficial veins in the chest could not be detected any more. Conclusion The therapy of intra-vascular stenting to treat SVS is microinvasive, simple and effective. Key words superior vana cava syndrome - tumor - stent - image diagnosis - interventional therapy
文摘Objective:To investigate the species prevalence of Enterococcus with their antimicrobial resistance pattern from patients of Dhaka Medical College Hospital.Methods:Samples were cultured and Enterococcus species were identified by conventional biochemical tests as well as PCR by using species specific primers for Enterococcus faecalis(E.faecalis)and Enterococcus faecium(E.faecium).For isolation of vancomycin resistant enterococci,minimum inhibitory concentration of vancomycin and PCR was done to detect vanA and vanB genes.Results:A total of 16 enterococci were isolated from 300 urine and 200 wound swab samples(15 from urine and 1 from wound swab)from July 2011 to June 2012.Enterococci were the third most common organism(8.47%)from urine after Escherichia coli(63.28%)and Enterobacter(11.87%).Out of 16 enterococci,10(62.5%)were E.faecalis,4(25%)were E.faecium and 2(12.5%)were other species.All the enterococci(100%)were sensitive to vancomycin and linezolid.Most of the strains were resistant to ciprofloxacin and azithromycin(87.5%),gentamycin(81.25%),ceftriaxone(75%),amoxiclav(31.25%)and imipenem(25%).E.faecium was more resistant than E.faecalis to azithromycin(100%),ciprofloxacin(100%),amoxiclav(75%)and imipenem(50%).No vancomycin resistant enterococci were identified and the range of minimum inhibitory concentration for vancomycin was 1-4μg/mL.None of the enterococci were positive for vanA and vanB genes.Conclusions:The presence of multidrug resistant enterococci should be considered as danger alarm for serious enterococcal infections and further study in large scale is needed.
