Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant g...Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant growth and leaf tonoplast V-H +-ATPase and V-H +-PPase activity. Increase of K + supply in the culture solution markedly increased the fresh weight, dry weight and K + content of S. salsa plants. Western blot analysis showed that the leaf V-H +-ATPase of S. salsa was at least composed of A,B,C,D,E and c subunits, and their expression decreased with the increase of NaCl concentration under K + starvation (12 μmol/L K +), but increased under normal K + application (6 mmol/L K +). Leaf V-H +-PPase molecular weight was about 72.6 kD and its expression increased as NaCl concentration increased under both high or low levels of K + concentration in nutrient solution. There was a positive correlation between of V-H +-ATPase or V-H +-PPase activity and the amounts of their expression. Results in this study suggest that K + nutrition plays an important role in the salt tolerance of S. salsa, and K + is involved in the regulation of V-H +-ATPase or V-H +-PPase activity under salt stress.展开更多
Cytosolic V-1-ATPase complex in pea (Pistum sativum L.) root cells was characterized by immunoblotting, immuno-electron microscopy and measurement of ATP hydrolysis activity. Using antibodies against the subunits A an...Cytosolic V-1-ATPase complex in pea (Pistum sativum L.) root cells was characterized by immunoblotting, immuno-electron microscopy and measurement of ATP hydrolysis activity. Using antibodies against the subunits A and B of V-type H+-ATPase (V-ATPase) from mung bean as primary antibodies, both immuno-blotting and immuno-electron microscopy showed that subunit A and subunit B existed in the cytosol. Measurement of ATPase activity further showed that cytosolic proteins had NO3--sensitive ATP hydrolysis activity. All these suggested that V-1-ATPase complex occurred in the cytosol. This is the direct proof that V-1-ATPase occurs in the cytosol of the plant cells for the first time.展开更多
文摘Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant growth and leaf tonoplast V-H +-ATPase and V-H +-PPase activity. Increase of K + supply in the culture solution markedly increased the fresh weight, dry weight and K + content of S. salsa plants. Western blot analysis showed that the leaf V-H +-ATPase of S. salsa was at least composed of A,B,C,D,E and c subunits, and their expression decreased with the increase of NaCl concentration under K + starvation (12 μmol/L K +), but increased under normal K + application (6 mmol/L K +). Leaf V-H +-PPase molecular weight was about 72.6 kD and its expression increased as NaCl concentration increased under both high or low levels of K + concentration in nutrient solution. There was a positive correlation between of V-H +-ATPase or V-H +-PPase activity and the amounts of their expression. Results in this study suggest that K + nutrition plays an important role in the salt tolerance of S. salsa, and K + is involved in the regulation of V-H +-ATPase or V-H +-PPase activity under salt stress.
文摘Cytosolic V-1-ATPase complex in pea (Pistum sativum L.) root cells was characterized by immunoblotting, immuno-electron microscopy and measurement of ATP hydrolysis activity. Using antibodies against the subunits A and B of V-type H+-ATPase (V-ATPase) from mung bean as primary antibodies, both immuno-blotting and immuno-electron microscopy showed that subunit A and subunit B existed in the cytosol. Measurement of ATPase activity further showed that cytosolic proteins had NO3--sensitive ATP hydrolysis activity. All these suggested that V-1-ATPase complex occurred in the cytosol. This is the direct proof that V-1-ATPase occurs in the cytosol of the plant cells for the first time.
